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Mass transfer effects on the reaction rate for heterogeneously distributed immobilized yeast cells

Gutenwik, Jan LU ; Nilsson, Bernt LU and Axelsson, Anders LU (2002) In Biotechnology and Bioengineering 79(6). p.664-673
Abstract
Here we examine the efficiency of different immobilized cell gradients applied to immobilized Saccharomyces cerevisiae fermenting glucose to ethanol. We developed a simulation model to fully study the competing effects of mass transfer hindrance and kinetics. It is based on a diffusion-reaction model and can be used to analyze the different cell concentration profiles inside an immobilized gel bead, in terms of effectiveness factors, productivity, and mass flux. The internal diffusion coefficient, which varies with the local cell concentration, as well as the external mass transfer, is taken into account when describing the efficiency. Although the diffusion hindrance is greater at higher cell concentrations, high cell concentration is... (More)
Here we examine the efficiency of different immobilized cell gradients applied to immobilized Saccharomyces cerevisiae fermenting glucose to ethanol. We developed a simulation model to fully study the competing effects of mass transfer hindrance and kinetics. It is based on a diffusion-reaction model and can be used to analyze the different cell concentration profiles inside an immobilized gel bead, in terms of effectiveness factors, productivity, and mass flux. The internal diffusion coefficient, which varies with the local cell concentration, as well as the external mass transfer, is taken into account when describing the efficiency. Although the diffusion hindrance is greater at higher cell concentrations, high cell concentration is still advantageous in the present case because the increase in reaction rate outweighs the diffusion hindrance. Thus, high cell concentrations contribute to increased productivity. The influence of the cell concentration gradient on the efficiency of the beads is negligible, Within the range of cell profiles studied it has been established that the location of the cells within the bead is of lesser importance. However, a steep cell gradient increases the importance of the external mass transfer. (C) 2002 Wiley Periodicals, Inc. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cell concentration gradient, variable, simulation, immobilized yeast cells, diffusion, mass transfer
in
Biotechnology and Bioengineering
volume
79
issue
6
pages
664 - 673
publisher
John Wiley & Sons
external identifiers
  • wos:000177549400010
  • pmid:12209814
  • scopus:0037144087
ISSN
1097-0290
DOI
10.1002/bit.10329
language
English
LU publication?
yes
id
c82c2fb6-970a-4fff-bbd8-5f2420ee6ab7 (old id 331096)
date added to LUP
2007-08-22 14:40:20
date last changed
2017-01-01 04:26:53
@article{c82c2fb6-970a-4fff-bbd8-5f2420ee6ab7,
  abstract     = {Here we examine the efficiency of different immobilized cell gradients applied to immobilized Saccharomyces cerevisiae fermenting glucose to ethanol. We developed a simulation model to fully study the competing effects of mass transfer hindrance and kinetics. It is based on a diffusion-reaction model and can be used to analyze the different cell concentration profiles inside an immobilized gel bead, in terms of effectiveness factors, productivity, and mass flux. The internal diffusion coefficient, which varies with the local cell concentration, as well as the external mass transfer, is taken into account when describing the efficiency. Although the diffusion hindrance is greater at higher cell concentrations, high cell concentration is still advantageous in the present case because the increase in reaction rate outweighs the diffusion hindrance. Thus, high cell concentrations contribute to increased productivity. The influence of the cell concentration gradient on the efficiency of the beads is negligible, Within the range of cell profiles studied it has been established that the location of the cells within the bead is of lesser importance. However, a steep cell gradient increases the importance of the external mass transfer. (C) 2002 Wiley Periodicals, Inc.},
  author       = {Gutenwik, Jan and Nilsson, Bernt and Axelsson, Anders},
  issn         = {1097-0290},
  keyword      = {cell concentration gradient,variable,simulation,immobilized yeast cells,diffusion,mass transfer},
  language     = {eng},
  number       = {6},
  pages        = {664--673},
  publisher    = {John Wiley & Sons},
  series       = {Biotechnology and Bioengineering},
  title        = {Mass transfer effects on the reaction rate for heterogeneously distributed immobilized yeast cells},
  url          = {http://dx.doi.org/10.1002/bit.10329},
  volume       = {79},
  year         = {2002},
}