Advanced

Strategies for enhancing fermentative production of glycerol - A review

Taherzadeh, MJ; Adler, L and Lidén, Gunnar LU (2002) In Enzyme and Microbial Technology 31(1-2). p.53-66
Abstract
The present paper reviews the metabolic basis of different methods for fermentative glycerol production. The most important microbial production organism is the yeast Saccharomyces cerevisiae but other yeast species, as well as molds, algae, and bacteria are of potential interest for glycerol production. A large variety of methods have been applied to increase the fermentative glycerol yield. The first methods were based on physiological control, e.g. chemically induced overproduction of glycerol through NADH entrapment by the addition of chemical steering agents (such as bisulfite). More recently, genetic engineering of the glycolytic pathway has been used to improve production, involving modulated function of e.g. triose phosphate... (More)
The present paper reviews the metabolic basis of different methods for fermentative glycerol production. The most important microbial production organism is the yeast Saccharomyces cerevisiae but other yeast species, as well as molds, algae, and bacteria are of potential interest for glycerol production. A large variety of methods have been applied to increase the fermentative glycerol yield. The first methods were based on physiological control, e.g. chemically induced overproduction of glycerol through NADH entrapment by the addition of chemical steering agents (such as bisulfite). More recently, genetic engineering of the glycolytic pathway has been used to improve production, involving modulated function of e.g. triose phosphate isomerase, phosphoglycerate mutase, PDC or alcohol dehydrogenase. Direct intervention in the glycerol pathway, such as overexpression of G3P dehydrogenase, has also been tried. The applied strategies can be divided into three principal groups; (a) deactivation or down-regulation of NADH oxidation sites alternative to G3P dehydrogenase, (b) increase of NADH generation or, (c) direct changes in the carbon flux to glycerol. (C) 2002 Published by Elsevier Science Inc. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
genetic engineering, NADH, redox, osmoregulation, yeast, glycerol
in
Enzyme and Microbial Technology
volume
31
issue
1-2
pages
53 - 66
publisher
Elsevier
external identifiers
  • wos:000177018400007
  • scopus:0036644259
ISSN
0141-0229
DOI
10.1016/S0141-0229(02)00069-8
language
English
LU publication?
yes
id
087f627a-e6d5-4c5a-ad84-ea215475706b (old id 332769)
date added to LUP
2007-11-15 12:45:25
date last changed
2017-09-10 03:35:47
@article{087f627a-e6d5-4c5a-ad84-ea215475706b,
  abstract     = {The present paper reviews the metabolic basis of different methods for fermentative glycerol production. The most important microbial production organism is the yeast Saccharomyces cerevisiae but other yeast species, as well as molds, algae, and bacteria are of potential interest for glycerol production. A large variety of methods have been applied to increase the fermentative glycerol yield. The first methods were based on physiological control, e.g. chemically induced overproduction of glycerol through NADH entrapment by the addition of chemical steering agents (such as bisulfite). More recently, genetic engineering of the glycolytic pathway has been used to improve production, involving modulated function of e.g. triose phosphate isomerase, phosphoglycerate mutase, PDC or alcohol dehydrogenase. Direct intervention in the glycerol pathway, such as overexpression of G3P dehydrogenase, has also been tried. The applied strategies can be divided into three principal groups; (a) deactivation or down-regulation of NADH oxidation sites alternative to G3P dehydrogenase, (b) increase of NADH generation or, (c) direct changes in the carbon flux to glycerol. (C) 2002 Published by Elsevier Science Inc.},
  author       = {Taherzadeh, MJ and Adler, L and Lidén, Gunnar},
  issn         = {0141-0229},
  keyword      = {genetic engineering,NADH,redox,osmoregulation,yeast,glycerol},
  language     = {eng},
  number       = {1-2},
  pages        = {53--66},
  publisher    = {Elsevier},
  series       = {Enzyme and Microbial Technology},
  title        = {Strategies for enhancing fermentative production of glycerol - A review},
  url          = {http://dx.doi.org/10.1016/S0141-0229(02)00069-8},
  volume       = {31},
  year         = {2002},
}