An immunocytochemical investigation of human trigeminal nucleus caudalis: CGRP, substance P and 5-HT1D-receptor immunoreactivities are expressed by trigeminal sensory fibres
(2002) In Cephalalgia 22(6). p.424-431- Abstract
- 5-HT1D (but not 5-HT1B )-receptor immunoreactivity (i.r.) can be detected on trigeminal fibres within the spinal trigeminal tract of the human brainstem. The present study used immunohistochemical and morphometric techniques to determine the proportions of trigeminal fibres expressing substance P, CGRP or 5-HT1D -receptor immunoreactivities. Co-localization studies between 5-HT1D -receptor and substance P- or CGRP-i.r. were also performed. Brainstem material was obtained with consent (four donors) and the total number of immunoreactive fibres within the trigeminal tract was estimated using random field sampling. A greater proportion of fibres (>1 mum diameter) expressed CGRP-i.r. (80 +/- 6%) compared with substance P-i.r. (46 +/- 7%) or... (More)
- 5-HT1D (but not 5-HT1B )-receptor immunoreactivity (i.r.) can be detected on trigeminal fibres within the spinal trigeminal tract of the human brainstem. The present study used immunohistochemical and morphometric techniques to determine the proportions of trigeminal fibres expressing substance P, CGRP or 5-HT1D -receptor immunoreactivities. Co-localization studies between 5-HT1D -receptor and substance P- or CGRP-i.r. were also performed. Brainstem material was obtained with consent (four donors) and the total number of immunoreactive fibres within the trigeminal tract was estimated using random field sampling. A greater proportion of fibres (>1 mum diameter) expressed CGRP-i.r. (80 +/- 6%) compared with substance P-i.r. (46 +/- 7%) or 5-HT1D -receptor-i.r. (25 +/- 1%). 5-HT1D -receptor-i.r. was co-localized on some CGRP- or substance P-i.r. fibres. This suggests that 5-HT1D -receptors can regulate the release of CGRP and substance P and may be relevant to the clinical effectiveness of 5-HT1B/1D -receptor agonists in the treatment of migraine and other cranial pain syndromes. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/333392
- author
- Smith, D ; Hill, RG ; Edvinsson, Lars LU and Longmore, J
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- migraine, brainstem, human, substance P, 5-HT1D-receptors, calcitonin gene-related peptide
- in
- Cephalalgia
- volume
- 22
- issue
- 6
- pages
- 424 - 431
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:12133041
- wos:000176919200003
- scopus:0036632586
- ISSN
- 0333-1024
- DOI
- 10.1046/j.1468-2982.2002.00378.x
- language
- English
- LU publication?
- yes
- id
- 0a82d271-27ed-4bac-a9a1-8ee5756b4242 (old id 333392)
- date added to LUP
- 2016-04-01 17:07:31
- date last changed
- 2024-01-11 21:08:49
@article{0a82d271-27ed-4bac-a9a1-8ee5756b4242,
abstract = {{5-HT1D (but not 5-HT1B )-receptor immunoreactivity (i.r.) can be detected on trigeminal fibres within the spinal trigeminal tract of the human brainstem. The present study used immunohistochemical and morphometric techniques to determine the proportions of trigeminal fibres expressing substance P, CGRP or 5-HT1D -receptor immunoreactivities. Co-localization studies between 5-HT1D -receptor and substance P- or CGRP-i.r. were also performed. Brainstem material was obtained with consent (four donors) and the total number of immunoreactive fibres within the trigeminal tract was estimated using random field sampling. A greater proportion of fibres (>1 mum diameter) expressed CGRP-i.r. (80 +/- 6%) compared with substance P-i.r. (46 +/- 7%) or 5-HT1D -receptor-i.r. (25 +/- 1%). 5-HT1D -receptor-i.r. was co-localized on some CGRP- or substance P-i.r. fibres. This suggests that 5-HT1D -receptors can regulate the release of CGRP and substance P and may be relevant to the clinical effectiveness of 5-HT1B/1D -receptor agonists in the treatment of migraine and other cranial pain syndromes.}},
author = {{Smith, D and Hill, RG and Edvinsson, Lars and Longmore, J}},
issn = {{0333-1024}},
keywords = {{migraine; brainstem; human; substance P; 5-HT1D-receptors; calcitonin gene-related peptide}},
language = {{eng}},
number = {{6}},
pages = {{424--431}},
publisher = {{Wiley-Blackwell}},
series = {{Cephalalgia}},
title = {{An immunocytochemical investigation of human trigeminal nucleus caudalis: CGRP, substance P and 5-HT1D-receptor immunoreactivities are expressed by trigeminal sensory fibres}},
url = {{http://dx.doi.org/10.1046/j.1468-2982.2002.00378.x}},
doi = {{10.1046/j.1468-2982.2002.00378.x}},
volume = {{22}},
year = {{2002}},
}