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An immunocytochemical investigation of human trigeminal nucleus caudalis: CGRP, substance P and 5-HT1D-receptor immunoreactivities are expressed by trigeminal sensory fibres

Smith, D; Hill, RG; Edvinsson, Lars LU and Longmore, J (2002) In Cephalalgia 22(6). p.424-431
Abstract
5-HT1D (but not 5-HT1B )-receptor immunoreactivity (i.r.) can be detected on trigeminal fibres within the spinal trigeminal tract of the human brainstem. The present study used immunohistochemical and morphometric techniques to determine the proportions of trigeminal fibres expressing substance P, CGRP or 5-HT1D -receptor immunoreactivities. Co-localization studies between 5-HT1D -receptor and substance P- or CGRP-i.r. were also performed. Brainstem material was obtained with consent (four donors) and the total number of immunoreactive fibres within the trigeminal tract was estimated using random field sampling. A greater proportion of fibres (>1 mum diameter) expressed CGRP-i.r. (80 +/- 6%) compared with substance P-i.r. (46 +/- 7%) or... (More)
5-HT1D (but not 5-HT1B )-receptor immunoreactivity (i.r.) can be detected on trigeminal fibres within the spinal trigeminal tract of the human brainstem. The present study used immunohistochemical and morphometric techniques to determine the proportions of trigeminal fibres expressing substance P, CGRP or 5-HT1D -receptor immunoreactivities. Co-localization studies between 5-HT1D -receptor and substance P- or CGRP-i.r. were also performed. Brainstem material was obtained with consent (four donors) and the total number of immunoreactive fibres within the trigeminal tract was estimated using random field sampling. A greater proportion of fibres (>1 mum diameter) expressed CGRP-i.r. (80 +/- 6%) compared with substance P-i.r. (46 +/- 7%) or 5-HT1D -receptor-i.r. (25 +/- 1%). 5-HT1D -receptor-i.r. was co-localized on some CGRP- or substance P-i.r. fibres. This suggests that 5-HT1D -receptors can regulate the release of CGRP and substance P and may be relevant to the clinical effectiveness of 5-HT1B/1D -receptor agonists in the treatment of migraine and other cranial pain syndromes. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
migraine, brainstem, human, substance P, 5-HT1D-receptors, calcitonin gene-related peptide
in
Cephalalgia
volume
22
issue
6
pages
424 - 431
publisher
Wiley-Blackwell
external identifiers
  • pmid:12133041
  • wos:000176919200003
  • scopus:0036632586
ISSN
0333-1024
DOI
10.1046/j.1468-2982.2002.00378.x
language
English
LU publication?
yes
id
0a82d271-27ed-4bac-a9a1-8ee5756b4242 (old id 333392)
date added to LUP
2007-11-14 12:44:37
date last changed
2017-11-19 04:15:02
@article{0a82d271-27ed-4bac-a9a1-8ee5756b4242,
  abstract     = {5-HT1D (but not 5-HT1B )-receptor immunoreactivity (i.r.) can be detected on trigeminal fibres within the spinal trigeminal tract of the human brainstem. The present study used immunohistochemical and morphometric techniques to determine the proportions of trigeminal fibres expressing substance P, CGRP or 5-HT1D -receptor immunoreactivities. Co-localization studies between 5-HT1D -receptor and substance P- or CGRP-i.r. were also performed. Brainstem material was obtained with consent (four donors) and the total number of immunoreactive fibres within the trigeminal tract was estimated using random field sampling. A greater proportion of fibres (>1 mum diameter) expressed CGRP-i.r. (80 +/- 6%) compared with substance P-i.r. (46 +/- 7%) or 5-HT1D -receptor-i.r. (25 +/- 1%). 5-HT1D -receptor-i.r. was co-localized on some CGRP- or substance P-i.r. fibres. This suggests that 5-HT1D -receptors can regulate the release of CGRP and substance P and may be relevant to the clinical effectiveness of 5-HT1B/1D -receptor agonists in the treatment of migraine and other cranial pain syndromes.},
  author       = {Smith, D and Hill, RG and Edvinsson, Lars and Longmore, J},
  issn         = {0333-1024},
  keyword      = {migraine,brainstem,human,substance P,5-HT1D-receptors,calcitonin gene-related peptide},
  language     = {eng},
  number       = {6},
  pages        = {424--431},
  publisher    = {Wiley-Blackwell},
  series       = {Cephalalgia},
  title        = {An immunocytochemical investigation of human trigeminal nucleus caudalis: CGRP, substance P and 5-HT1D-receptor immunoreactivities are expressed by trigeminal sensory fibres},
  url          = {http://dx.doi.org/10.1046/j.1468-2982.2002.00378.x},
  volume       = {22},
  year         = {2002},
}