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Limitations in the use of 3-hydroxy fatty acid analysis to determine endotoxin in mammalian samples

Szponar, Bogumila LU ; Norin, E; Midtvedt, T and Larsson, Lennart LU (2002) In Journal of Microbiological Methods 50(3). p.283-289
Abstract
3-Hydroxy fatty acids (3-OH FAs) of 10-18-carbon chain lengths are constituents of the lipopolysaccharide of Gram-negative bacteria. These acids are used as chemical markers for determining endotoxin in environmental samples. The present communication addresses the question whether this type of analysis also would be applicable to mammalian samples. Low levels (6.1 +/- 1.6-94.0 +/- 23.2 pmol/ml) of the studied 3-OH FAs were detected in blood from both conventional and germ-fine rats. The levels were considerably higher (0.0-1.06 +/- 0.17 nmol/mg) in livers. The amounts of the 3-OH FAs did not differ between the two groups of rats. All analyses were made by gas chromatography-tandem mass spectrometry (GC-MSMS) for unequivocal... (More)
3-Hydroxy fatty acids (3-OH FAs) of 10-18-carbon chain lengths are constituents of the lipopolysaccharide of Gram-negative bacteria. These acids are used as chemical markers for determining endotoxin in environmental samples. The present communication addresses the question whether this type of analysis also would be applicable to mammalian samples. Low levels (6.1 +/- 1.6-94.0 +/- 23.2 pmol/ml) of the studied 3-OH FAs were detected in blood from both conventional and germ-fine rats. The levels were considerably higher (0.0-1.06 +/- 0.17 nmol/mg) in livers. The amounts of the 3-OH FAs did not differ between the two groups of rats. All analyses were made by gas chromatography-tandem mass spectrometry (GC-MSMS) for unequivocal identification. The results illustrate a limitation in using 3-OH FA analysis to determine endotoxin in mammalian samples since these acids may represent not only endotoxin but also products from mammalian mitochondrial fatty acid beta-oxidation. (C) 2002 Elsevier Science B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
germ-free, animals, microbial marker, GC-MSMS, 3-hydroxy fatty acids, endotoxin
in
Journal of Microbiological Methods
volume
50
issue
3
pages
283 - 289
publisher
Elsevier
external identifiers
  • wos:000176345500007
  • pmid:12031578
  • scopus:0036273710
ISSN
1872-8359
DOI
10.1016/S0167-7012(02)00038-6
language
English
LU publication?
yes
id
2d5cd5f7-f852-42d8-9352-835ad499964d (old id 334807)
date added to LUP
2007-11-15 12:36:28
date last changed
2017-09-17 05:03:15
@article{2d5cd5f7-f852-42d8-9352-835ad499964d,
  abstract     = {3-Hydroxy fatty acids (3-OH FAs) of 10-18-carbon chain lengths are constituents of the lipopolysaccharide of Gram-negative bacteria. These acids are used as chemical markers for determining endotoxin in environmental samples. The present communication addresses the question whether this type of analysis also would be applicable to mammalian samples. Low levels (6.1 +/- 1.6-94.0 +/- 23.2 pmol/ml) of the studied 3-OH FAs were detected in blood from both conventional and germ-fine rats. The levels were considerably higher (0.0-1.06 +/- 0.17 nmol/mg) in livers. The amounts of the 3-OH FAs did not differ between the two groups of rats. All analyses were made by gas chromatography-tandem mass spectrometry (GC-MSMS) for unequivocal identification. The results illustrate a limitation in using 3-OH FA analysis to determine endotoxin in mammalian samples since these acids may represent not only endotoxin but also products from mammalian mitochondrial fatty acid beta-oxidation. (C) 2002 Elsevier Science B.V. All rights reserved.},
  author       = {Szponar, Bogumila and Norin, E and Midtvedt, T and Larsson, Lennart},
  issn         = {1872-8359},
  keyword      = {germ-free,animals,microbial marker,GC-MSMS,3-hydroxy fatty acids,endotoxin},
  language     = {eng},
  number       = {3},
  pages        = {283--289},
  publisher    = {Elsevier},
  series       = {Journal of Microbiological Methods},
  title        = {Limitations in the use of 3-hydroxy fatty acid analysis to determine endotoxin in mammalian samples},
  url          = {http://dx.doi.org/10.1016/S0167-7012(02)00038-6},
  volume       = {50},
  year         = {2002},
}