Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Towards molecular-imprint based SPE of local anaesthetics

Andersson, LI ; Abdel-Rehim, M ; Nicklasson, L ; Schweitz, Leif LU and Nilsson, Staffan LU (2002) In Chromatographia 55. p.65-69
Abstract
The use of imprinted polymers for selective solid-phase extraction of local anaesthetics from human plasma was investigated. Methacrylic acid-ethylene glycol dimethacrylate based MIPs were developed by the alternative template approach using a structural analogue, pentycaine, as the template. The influence of the type and concentration of detergent in the buffer on selective and non-specific binding was investigated. Tween 20, Triton X-100 and Brij 35 were found to eliminate non-specific adsorption and leave selective imprint-onalyte binding essentially unaffected. Efficient pre-concert ration of ropivacaine and bupivacaine from plasma samples, both off-line prior to GC and in-line with LC-MS-MS, was demonstrated.
Please use this url to cite or link to this publication:
author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
anaesthetics, solid-phase extraction, molecular imprinting
in
Chromatographia
volume
55
pages
65 - 69
publisher
Vieweg Verlag
external identifiers
  • wos:000176186700013
  • scopus:0036100286
ISSN
0009-5893
DOI
10.1007/BF02493355
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
id
d6cbb3b3-1533-426f-9454-bddab934318a (old id 335565)
date added to LUP
2016-04-01 16:54:14
date last changed
2022-01-28 23:01:06
@article{d6cbb3b3-1533-426f-9454-bddab934318a,
  abstract     = {{The use of imprinted polymers for selective solid-phase extraction of local anaesthetics from human plasma was investigated. Methacrylic acid-ethylene glycol dimethacrylate based MIPs were developed by the alternative template approach using a structural analogue, pentycaine, as the template. The influence of the type and concentration of detergent in the buffer on selective and non-specific binding was investigated. Tween 20, Triton X-100 and Brij 35 were found to eliminate non-specific adsorption and leave selective imprint-onalyte binding essentially unaffected. Efficient pre-concert ration of ropivacaine and bupivacaine from plasma samples, both off-line prior to GC and in-line with LC-MS-MS, was demonstrated.}},
  author       = {{Andersson, LI and Abdel-Rehim, M and Nicklasson, L and Schweitz, Leif and Nilsson, Staffan}},
  issn         = {{0009-5893}},
  keywords     = {{anaesthetics; solid-phase extraction; molecular imprinting}},
  language     = {{eng}},
  pages        = {{65--69}},
  publisher    = {{Vieweg Verlag}},
  series       = {{Chromatographia}},
  title        = {{Towards molecular-imprint based SPE of local anaesthetics}},
  url          = {{http://dx.doi.org/10.1007/BF02493355}},
  doi          = {{10.1007/BF02493355}},
  volume       = {{55}},
  year         = {{2002}},
}