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Towards molecular-imprint based SPE of local anaesthetics

Andersson, LI; Abdel-Rehim, M; Nicklasson, L; Schweitz, Leif LU and Nilsson, Staffan LU (2002) In Chromatographia 55. p.65-69
Abstract
The use of imprinted polymers for selective solid-phase extraction of local anaesthetics from human plasma was investigated. Methacrylic acid-ethylene glycol dimethacrylate based MIPs were developed by the alternative template approach using a structural analogue, pentycaine, as the template. The influence of the type and concentration of detergent in the buffer on selective and non-specific binding was investigated. Tween 20, Triton X-100 and Brij 35 were found to eliminate non-specific adsorption and leave selective imprint-onalyte binding essentially unaffected. Efficient pre-concert ration of ropivacaine and bupivacaine from plasma samples, both off-line prior to GC and in-line with LC-MS-MS, was demonstrated.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
anaesthetics, solid-phase extraction, molecular imprinting
in
Chromatographia
volume
55
pages
65 - 69
publisher
Vieweg Verlag
external identifiers
  • wos:000176186700013
  • scopus:0036100286
ISSN
0009-5893
DOI
10.1007/BF02493355
language
English
LU publication?
yes
id
d6cbb3b3-1533-426f-9454-bddab934318a (old id 335565)
date added to LUP
2007-08-07 09:32:43
date last changed
2017-03-19 04:12:39
@article{d6cbb3b3-1533-426f-9454-bddab934318a,
  abstract     = {The use of imprinted polymers for selective solid-phase extraction of local anaesthetics from human plasma was investigated. Methacrylic acid-ethylene glycol dimethacrylate based MIPs were developed by the alternative template approach using a structural analogue, pentycaine, as the template. The influence of the type and concentration of detergent in the buffer on selective and non-specific binding was investigated. Tween 20, Triton X-100 and Brij 35 were found to eliminate non-specific adsorption and leave selective imprint-onalyte binding essentially unaffected. Efficient pre-concert ration of ropivacaine and bupivacaine from plasma samples, both off-line prior to GC and in-line with LC-MS-MS, was demonstrated.},
  author       = {Andersson, LI and Abdel-Rehim, M and Nicklasson, L and Schweitz, Leif and Nilsson, Staffan},
  issn         = {0009-5893},
  keyword      = {anaesthetics,solid-phase extraction,molecular imprinting},
  language     = {eng},
  pages        = {65--69},
  publisher    = {Vieweg Verlag},
  series       = {Chromatographia},
  title        = {Towards molecular-imprint based SPE of local anaesthetics},
  url          = {http://dx.doi.org/10.1007/BF02493355},
  volume       = {55},
  year         = {2002},
}