Towards molecular-imprint based SPE of local anaesthetics
(2002) In Chromatographia 55. p.65-69- Abstract
- The use of imprinted polymers for selective solid-phase extraction of local anaesthetics from human plasma was investigated. Methacrylic acid-ethylene glycol dimethacrylate based MIPs were developed by the alternative template approach using a structural analogue, pentycaine, as the template. The influence of the type and concentration of detergent in the buffer on selective and non-specific binding was investigated. Tween 20, Triton X-100 and Brij 35 were found to eliminate non-specific adsorption and leave selective imprint-onalyte binding essentially unaffected. Efficient pre-concert ration of ropivacaine and bupivacaine from plasma samples, both off-line prior to GC and in-line with LC-MS-MS, was demonstrated.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/335565
- author
- Andersson, LI ; Abdel-Rehim, M ; Nicklasson, L ; Schweitz, Leif LU and Nilsson, Staffan LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- anaesthetics, solid-phase extraction, molecular imprinting
- in
- Chromatographia
- volume
- 55
- pages
- 65 - 69
- publisher
- Vieweg Verlag
- external identifiers
-
- wos:000176186700013
- scopus:0036100286
- ISSN
- 0009-5893
- DOI
- 10.1007/BF02493355
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- d6cbb3b3-1533-426f-9454-bddab934318a (old id 335565)
- date added to LUP
- 2016-04-01 16:54:14
- date last changed
- 2022-01-28 23:01:06
@article{d6cbb3b3-1533-426f-9454-bddab934318a, abstract = {{The use of imprinted polymers for selective solid-phase extraction of local anaesthetics from human plasma was investigated. Methacrylic acid-ethylene glycol dimethacrylate based MIPs were developed by the alternative template approach using a structural analogue, pentycaine, as the template. The influence of the type and concentration of detergent in the buffer on selective and non-specific binding was investigated. Tween 20, Triton X-100 and Brij 35 were found to eliminate non-specific adsorption and leave selective imprint-onalyte binding essentially unaffected. Efficient pre-concert ration of ropivacaine and bupivacaine from plasma samples, both off-line prior to GC and in-line with LC-MS-MS, was demonstrated.}}, author = {{Andersson, LI and Abdel-Rehim, M and Nicklasson, L and Schweitz, Leif and Nilsson, Staffan}}, issn = {{0009-5893}}, keywords = {{anaesthetics; solid-phase extraction; molecular imprinting}}, language = {{eng}}, pages = {{65--69}}, publisher = {{Vieweg Verlag}}, series = {{Chromatographia}}, title = {{Towards molecular-imprint based SPE of local anaesthetics}}, url = {{http://dx.doi.org/10.1007/BF02493355}}, doi = {{10.1007/BF02493355}}, volume = {{55}}, year = {{2002}}, }