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Removal of the fermentation by-product succinyl L-tyrosine from the beta-lactamase inhibitor clavulanic acid using a molecularly imprinted polymer

Yu, Yihua LU ; Ye, Lei LU ; de Biasi, V and Mosbach, Klaus LU (2002) In Biotechnology and Bioengineering 79(1). p.23-28
Abstract
Clavulanic acid is a beta-lactamase inhibitor used in therapeutic combinations with the penicillin-type antibiotics. During the fermentation leading to clavulanic acid, a succinyl L-tyrosine by-product is unavoidably formed. Occasionally, the amount of this by-product is found to be as high as 2% of the product even after standard purification operations. To further remove this impurity, we prepared a highly specific adsorbent for succinyl L-tyrosine with the molecular imprinting technique. This was performed by simultaneously using vinylbenzyl trimethylammonium chloride and methacrylic acid as the functional monomers. The imprinted polymer selectively bound succinyl L-tyrosine, and could be successfully used to remove this impurity at... (More)
Clavulanic acid is a beta-lactamase inhibitor used in therapeutic combinations with the penicillin-type antibiotics. During the fermentation leading to clavulanic acid, a succinyl L-tyrosine by-product is unavoidably formed. Occasionally, the amount of this by-product is found to be as high as 2% of the product even after standard purification operations. To further remove this impurity, we prepared a highly specific adsorbent for succinyl L-tyrosine with the molecular imprinting technique. This was performed by simultaneously using vinylbenzyl trimethylammonium chloride and methacrylic acid as the functional monomers. The imprinted polymer selectively bound succinyl L-tyrosine, and could be successfully used to remove this impurity at concentrations of less than 2% in the presence of clavulanic acid. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
molecularly imprinted polymer, succinyl L-tyrosine, clavulanic acid, purification
in
Biotechnology and Bioengineering
volume
79
issue
1
pages
23 - 28
publisher
John Wiley & Sons
external identifiers
  • wos:000176179700004
  • scopus:0037025205
ISSN
1097-0290
DOI
10.1002/bit.10271
language
English
LU publication?
yes
id
dfe2b9a4-6732-4183-8dbb-f7c4112e1ad0 (old id 335590)
date added to LUP
2007-11-19 12:28:22
date last changed
2017-01-01 04:46:55
@article{dfe2b9a4-6732-4183-8dbb-f7c4112e1ad0,
  abstract     = {Clavulanic acid is a beta-lactamase inhibitor used in therapeutic combinations with the penicillin-type antibiotics. During the fermentation leading to clavulanic acid, a succinyl L-tyrosine by-product is unavoidably formed. Occasionally, the amount of this by-product is found to be as high as 2% of the product even after standard purification operations. To further remove this impurity, we prepared a highly specific adsorbent for succinyl L-tyrosine with the molecular imprinting technique. This was performed by simultaneously using vinylbenzyl trimethylammonium chloride and methacrylic acid as the functional monomers. The imprinted polymer selectively bound succinyl L-tyrosine, and could be successfully used to remove this impurity at concentrations of less than 2% in the presence of clavulanic acid.},
  author       = {Yu, Yihua and Ye, Lei and de Biasi, V and Mosbach, Klaus},
  issn         = {1097-0290},
  keyword      = {molecularly imprinted polymer,succinyl L-tyrosine,clavulanic acid,purification},
  language     = {eng},
  number       = {1},
  pages        = {23--28},
  publisher    = {John Wiley & Sons},
  series       = {Biotechnology and Bioengineering},
  title        = {Removal of the fermentation by-product succinyl L-tyrosine from the beta-lactamase inhibitor clavulanic acid using a molecularly imprinted polymer},
  url          = {http://dx.doi.org/10.1002/bit.10271},
  volume       = {79},
  year         = {2002},
}