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DNA-lipid systems. A physical chemistry study

Dias, R; Antunes, F; Miguel, M; Lindman, Stina LU and Lindman, Björn LU (2002) In Brazilian Journal of Medical and Biological Research 35(5). p.509-522
Abstract
It is well known that the interaction of polyelectrolytes with oppositely charged surfactants leads to an associative phase separation; however, the phase behavior of DNA and oppositely charged surfactants is more strongly associative than observed in other systems. A precipitate is formed with very low amounts of surfactant and DNA. DNA compaction is a general phenomenon in the presence of multivalent ions and positively charged surfaces; because of the high charge density there are strong attractive ion correlation effects. Techniques like phase diagram determinations, fluorescence microscopy, and ellipsometry were used to study these systems. The interaction between DNA and catanionic mixtures (i.e., mixtures of cationic and anionic... (More)
It is well known that the interaction of polyelectrolytes with oppositely charged surfactants leads to an associative phase separation; however, the phase behavior of DNA and oppositely charged surfactants is more strongly associative than observed in other systems. A precipitate is formed with very low amounts of surfactant and DNA. DNA compaction is a general phenomenon in the presence of multivalent ions and positively charged surfaces; because of the high charge density there are strong attractive ion correlation effects. Techniques like phase diagram determinations, fluorescence microscopy, and ellipsometry were used to study these systems. The interaction between DNA and catanionic mixtures (i.e., mixtures of cationic and anionic surfactants) was also investigated. We observed that DNA compacts and adsorbs onto the surface of positively charged vesicles, and that the addition of an anionic surfactant can release DNA back into solution from a compact globular complex between DNA and the cationic surfactant. Finally, DNA interactions with polycations, chitosans with different chain lengths, were studied by fluorescence microscopy, in vivo transfection assays and cryogenic transmission electron microscopy. The general conclusion is that a chitosan effective in promoting compaction is also efficient in transfection. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
behavior, phase, chitosan, catanionic mixtures, DNA, cationic surfactants, polyelectrolyte-oppositely charged surfactant systems
in
Brazilian Journal of Medical and Biological Research
volume
35
issue
5
pages
509 - 522
publisher
ASSOC BRAS DIVULG CIENTIFICA
external identifiers
  • wos:000175800900002
  • pmid:12011935
  • scopus:0036266660
ISSN
0100-879X
language
English
LU publication?
yes
id
eb96eadb-63ad-4bbb-b3bc-465042fb511d (old id 337273)
alternative location
http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0100-879X2002000500002&lng=en&nrm=iso&tlng=en
date added to LUP
2007-08-16 09:05:49
date last changed
2017-12-10 03:48:57
@article{eb96eadb-63ad-4bbb-b3bc-465042fb511d,
  abstract     = {It is well known that the interaction of polyelectrolytes with oppositely charged surfactants leads to an associative phase separation; however, the phase behavior of DNA and oppositely charged surfactants is more strongly associative than observed in other systems. A precipitate is formed with very low amounts of surfactant and DNA. DNA compaction is a general phenomenon in the presence of multivalent ions and positively charged surfaces; because of the high charge density there are strong attractive ion correlation effects. Techniques like phase diagram determinations, fluorescence microscopy, and ellipsometry were used to study these systems. The interaction between DNA and catanionic mixtures (i.e., mixtures of cationic and anionic surfactants) was also investigated. We observed that DNA compacts and adsorbs onto the surface of positively charged vesicles, and that the addition of an anionic surfactant can release DNA back into solution from a compact globular complex between DNA and the cationic surfactant. Finally, DNA interactions with polycations, chitosans with different chain lengths, were studied by fluorescence microscopy, in vivo transfection assays and cryogenic transmission electron microscopy. The general conclusion is that a chitosan effective in promoting compaction is also efficient in transfection.},
  author       = {Dias, R and Antunes, F and Miguel, M and Lindman, Stina and Lindman, Björn},
  issn         = {0100-879X},
  keyword      = {behavior,phase,chitosan,catanionic mixtures,DNA,cationic surfactants,polyelectrolyte-oppositely charged surfactant systems},
  language     = {eng},
  number       = {5},
  pages        = {509--522},
  publisher    = {ASSOC BRAS DIVULG CIENTIFICA},
  series       = {Brazilian Journal of Medical and Biological Research},
  title        = {DNA-lipid systems. A physical chemistry study},
  volume       = {35},
  year         = {2002},
}