Advanced

Molecularly imprinted polymers for histamine recognition in aqueous environment

Trikka, Foteini A.; Yoshimatsu, Keiichi LU ; Ye, Lei LU and Kyriakidis, Dimitrios A. (2012) In Amino Acids 43(5). p.2113-2124
Abstract
Molecularly imprinted polymers (MIP) for histamine using methacrylic acid were developed and recognition mechanisms were thoroughly characterized for the first time in this study. The binding affinity of imprinted polymer with structurally related compounds was studied in organic and aqueous media, at various conditions. In organic media, MIP was found to bind histamine two and six times more than ranitidine and fluoxetine, respectively, whereas higher selectivity was observed in the case of dimentidene or disodium cromoglycate. The specific binding sites of MIP recognized histamine over l-histidine in aqueous conditions, while higher affinity for histamine compared to ranitidine, disodium cromoglycate, putrescine and to a putrescine... (More)
Molecularly imprinted polymers (MIP) for histamine using methacrylic acid were developed and recognition mechanisms were thoroughly characterized for the first time in this study. The binding affinity of imprinted polymer with structurally related compounds was studied in organic and aqueous media, at various conditions. In organic media, MIP was found to bind histamine two and six times more than ranitidine and fluoxetine, respectively, whereas higher selectivity was observed in the case of dimentidene or disodium cromoglycate. The specific binding sites of MIP recognized histamine over l-histidine in aqueous conditions, while higher affinity for histamine compared to ranitidine, disodium cromoglycate, putrescine and to a putrescine analogue was observed. A combination of NMR and UV spectroscopy analyses for investigation of imprinting and recognition properties revealed that strong specific interactions between the functional monomer and histamine in the prepolymerization and in the aqueous solutions were probably responsible for histamine recognition. The preparation of histamine MIPs and elucidation of imprinting and recognition mechanism may serve as useful insight for future application of MIPs. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Aqueous assay, Histamine, Molecular imprinting, Molecular recognition
in
Amino Acids
volume
43
issue
5
pages
2113 - 2124
publisher
Springer
external identifiers
  • wos:000309863400027
  • scopus:84868156594
ISSN
0939-4451
DOI
10.1007/s00726-012-1297-8
language
English
LU publication?
yes
id
c7f59b58-79ca-493a-ba2d-96ba14031892 (old id 3388266)
date added to LUP
2013-01-21 09:13:35
date last changed
2017-09-24 03:12:23
@article{c7f59b58-79ca-493a-ba2d-96ba14031892,
  abstract     = {Molecularly imprinted polymers (MIP) for histamine using methacrylic acid were developed and recognition mechanisms were thoroughly characterized for the first time in this study. The binding affinity of imprinted polymer with structurally related compounds was studied in organic and aqueous media, at various conditions. In organic media, MIP was found to bind histamine two and six times more than ranitidine and fluoxetine, respectively, whereas higher selectivity was observed in the case of dimentidene or disodium cromoglycate. The specific binding sites of MIP recognized histamine over l-histidine in aqueous conditions, while higher affinity for histamine compared to ranitidine, disodium cromoglycate, putrescine and to a putrescine analogue was observed. A combination of NMR and UV spectroscopy analyses for investigation of imprinting and recognition properties revealed that strong specific interactions between the functional monomer and histamine in the prepolymerization and in the aqueous solutions were probably responsible for histamine recognition. The preparation of histamine MIPs and elucidation of imprinting and recognition mechanism may serve as useful insight for future application of MIPs.},
  author       = {Trikka, Foteini A. and Yoshimatsu, Keiichi and Ye, Lei and Kyriakidis, Dimitrios A.},
  issn         = {0939-4451},
  keyword      = {Aqueous assay,Histamine,Molecular imprinting,Molecular recognition},
  language     = {eng},
  number       = {5},
  pages        = {2113--2124},
  publisher    = {Springer},
  series       = {Amino Acids},
  title        = {Molecularly imprinted polymers for histamine recognition in aqueous environment},
  url          = {http://dx.doi.org/10.1007/s00726-012-1297-8},
  volume       = {43},
  year         = {2012},
}