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A stable liquid-liquid extraction system for clavulanic acid using polymer-based aqueous two-phase systems

Pereira, Jorge F. B.; Santos, Valeria C.; Johansson, Hans-Olof LU ; Teixeira, Jose A. C. and Pessoa Jr., Adalberto (2012) In Separation and Purification Technology 98. p.441-450
Abstract
The partitioning of Clavulanic Acid (CA) in a novel inexpensive and stable aqueous two-phase system (ATPS) composed by poly(ethylene glycol) (PEG) and sodium polyacrylate (NaPA) has been studied. The aqueous two-phase systems are formed by mixing both polymers with a salt (NaCl or Na2SO4) and an aqueous solution of CA. The stability of CA on the presence of both polymers was investigated and it was observed that these polymers do not degrade the biomolecule. The effect of PEG-molecular size, polymer concentrations on the commercial CA partitioning has been studied, at 25 degrees C. The data showed that commercial CA was preferentially partitioned for the PEG-rich phase with a partition coefficient (K-CA) between 1 and 12 in the PEG/NaPA... (More)
The partitioning of Clavulanic Acid (CA) in a novel inexpensive and stable aqueous two-phase system (ATPS) composed by poly(ethylene glycol) (PEG) and sodium polyacrylate (NaPA) has been studied. The aqueous two-phase systems are formed by mixing both polymers with a salt (NaCl or Na2SO4) and an aqueous solution of CA. The stability of CA on the presence of both polymers was investigated and it was observed that these polymers do not degrade the biomolecule. The effect of PEG-molecular size, polymer concentrations on the commercial CA partitioning has been studied, at 25 degrees C. The data showed that commercial CA was preferentially partitioned for the PEG-rich phase with a partition coefficient (K-CA) between 1 and 12 in the PEG/NaPA aqueous two phase systems supplemented with NaCl and Na2SO4. The partition to the PEG phase was increased in the systems with high polymer concentrations. Furthermore, Na2SO4 caused higher CA preference for the PEG-phase than NaCl. The systems having a composition with 10 wt.% of PEG4000, 20 wt.% of NaPA8000 and 6 wt.% of Na2SO4 were selected as the optimal ones in terms of recovery of CA from fermented broth of Streptomyces clavuligerus. The partitioning results (K-CA = 9.15 +/- 1.06) are competitive with commercial extraction methods of CA (K-CA = 11.91 +/- 2.08) which emphasizes that the system PEG/NaPA/Na2SO4 can be used as a new process to CA purification/concentration from fermented broth. (C) 2012 Elsevier B.V. All rights reserved. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
Clavulanic acid, Aqueous two-phase systems, Poly(ethylene glycol), Poly(acrylic acid), Streptomyces clavuligerus
in
Separation and Purification Technology
volume
98
pages
441 - 450
publisher
Elsevier
external identifiers
  • wos:000311247500056
  • scopus:84865818502
ISSN
1873-3794
DOI
10.1016/j.seppur.2012.08.008
language
English
LU publication?
yes
id
42b48678-29be-4f9f-8896-4f74850b5ff6 (old id 3388606)
date added to LUP
2013-01-30 13:34:16
date last changed
2017-08-06 03:26:40
@article{42b48678-29be-4f9f-8896-4f74850b5ff6,
  abstract     = {The partitioning of Clavulanic Acid (CA) in a novel inexpensive and stable aqueous two-phase system (ATPS) composed by poly(ethylene glycol) (PEG) and sodium polyacrylate (NaPA) has been studied. The aqueous two-phase systems are formed by mixing both polymers with a salt (NaCl or Na2SO4) and an aqueous solution of CA. The stability of CA on the presence of both polymers was investigated and it was observed that these polymers do not degrade the biomolecule. The effect of PEG-molecular size, polymer concentrations on the commercial CA partitioning has been studied, at 25 degrees C. The data showed that commercial CA was preferentially partitioned for the PEG-rich phase with a partition coefficient (K-CA) between 1 and 12 in the PEG/NaPA aqueous two phase systems supplemented with NaCl and Na2SO4. The partition to the PEG phase was increased in the systems with high polymer concentrations. Furthermore, Na2SO4 caused higher CA preference for the PEG-phase than NaCl. The systems having a composition with 10 wt.% of PEG4000, 20 wt.% of NaPA8000 and 6 wt.% of Na2SO4 were selected as the optimal ones in terms of recovery of CA from fermented broth of Streptomyces clavuligerus. The partitioning results (K-CA = 9.15 +/- 1.06) are competitive with commercial extraction methods of CA (K-CA = 11.91 +/- 2.08) which emphasizes that the system PEG/NaPA/Na2SO4 can be used as a new process to CA purification/concentration from fermented broth. (C) 2012 Elsevier B.V. All rights reserved.},
  author       = {Pereira, Jorge F. B. and Santos, Valeria C. and Johansson, Hans-Olof and Teixeira, Jose A. C. and Pessoa Jr., Adalberto},
  issn         = {1873-3794},
  keyword      = {Clavulanic acid,Aqueous two-phase systems,Poly(ethylene glycol),Poly(acrylic acid),Streptomyces clavuligerus},
  language     = {eng},
  pages        = {441--450},
  publisher    = {Elsevier},
  series       = {Separation and Purification Technology},
  title        = {A stable liquid-liquid extraction system for clavulanic acid using polymer-based aqueous two-phase systems},
  url          = {http://dx.doi.org/10.1016/j.seppur.2012.08.008},
  volume       = {98},
  year         = {2012},
}