AKT1 induces Nanog promoter in a SUMOylation-dependent manner in different pluripotent contexts
(2023) In BMC Research Notes 16(1).- Abstract
AKT/PKB is a kinase crucial for pluripotency maintenance in pluripotent stem cells. Multiple post-translational modifications modulate its activity. We have previously demonstrated that AKT1 induces the expression of the pluripotency transcription factor Nanog in a SUMOylation-dependent manner in mouse embryonic stem cells. Here, we studied different cellular contexts and main candidates that could mediate this induction. Our results strongly suggest the pluripotency transcription factors OCT4 and SOX2 are not essential mediators. Additionally, we concluded that this induction takes place in different pluripotent contexts but not in terminally differentiated cells. Finally, the cross-matching analysis of ESCs, iPSCs and MEFs... (More)
AKT/PKB is a kinase crucial for pluripotency maintenance in pluripotent stem cells. Multiple post-translational modifications modulate its activity. We have previously demonstrated that AKT1 induces the expression of the pluripotency transcription factor Nanog in a SUMOylation-dependent manner in mouse embryonic stem cells. Here, we studied different cellular contexts and main candidates that could mediate this induction. Our results strongly suggest the pluripotency transcription factors OCT4 and SOX2 are not essential mediators. Additionally, we concluded that this induction takes place in different pluripotent contexts but not in terminally differentiated cells. Finally, the cross-matching analysis of ESCs, iPSCs and MEFs transcriptomes and AKT1 phosphorylation targets provided new clues about possible factors that could be involved in the SUMOylation-dependent Nanog induction by AKT.
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- author
- Francia, Marcos Gabriel ; Verneri, Paula ; Oses, Camila ; Vazquez Echegaray, Camila LU ; Garcia, Mora Reneé ; Toro, Ayelen ; Levi, Valeria and Guberman, Alejandra Sonia
- organization
- publishing date
- 2023
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- AKT1 SUMO conjugation, E17K AKT1 mutant, Embryonic stem cells, Induced pluripotent stem cells, MEF, U-2 OS, UBC9(C93S)
- in
- BMC Research Notes
- volume
- 16
- issue
- 1
- article number
- 309
- publisher
- BioMed Central (BMC)
- external identifiers
-
- pmid:37919788
- scopus:85175704647
- ISSN
- 1756-0500
- DOI
- 10.1186/s13104-023-06598-3
- language
- English
- LU publication?
- yes
- id
- 33945036-98a2-4a67-8ae4-2e7640a953f0
- date added to LUP
- 2023-11-24 14:09:28
- date last changed
- 2024-04-21 16:59:49
@article{33945036-98a2-4a67-8ae4-2e7640a953f0, abstract = {{<p>AKT/PKB is a kinase crucial for pluripotency maintenance in pluripotent stem cells. Multiple post-translational modifications modulate its activity. We have previously demonstrated that AKT1 induces the expression of the pluripotency transcription factor Nanog in a SUMOylation-dependent manner in mouse embryonic stem cells. Here, we studied different cellular contexts and main candidates that could mediate this induction. Our results strongly suggest the pluripotency transcription factors OCT4 and SOX2 are not essential mediators. Additionally, we concluded that this induction takes place in different pluripotent contexts but not in terminally differentiated cells. Finally, the cross-matching analysis of ESCs, iPSCs and MEFs transcriptomes and AKT1 phosphorylation targets provided new clues about possible factors that could be involved in the SUMOylation-dependent Nanog induction by AKT.</p>}}, author = {{Francia, Marcos Gabriel and Verneri, Paula and Oses, Camila and Vazquez Echegaray, Camila and Garcia, Mora Reneé and Toro, Ayelen and Levi, Valeria and Guberman, Alejandra Sonia}}, issn = {{1756-0500}}, keywords = {{AKT1 SUMO conjugation; E17K AKT1 mutant; Embryonic stem cells; Induced pluripotent stem cells; MEF; U-2 OS; UBC9(C93S)}}, language = {{eng}}, number = {{1}}, publisher = {{BioMed Central (BMC)}}, series = {{BMC Research Notes}}, title = {{AKT1 induces Nanog promoter in a SUMOylation-dependent manner in different pluripotent contexts}}, url = {{http://dx.doi.org/10.1186/s13104-023-06598-3}}, doi = {{10.1186/s13104-023-06598-3}}, volume = {{16}}, year = {{2023}}, }