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Modeling simultaneous saccharification and fermentation of softwood

Pettersson, PO; Eklund, R and Zacchi, Guido LU (2002) In Applied Biochemistry and Biotechnology 98. p.733-746
Abstract
Simultaneous saccharification and fermentation (SSF) of wood has been modeled for the past 15-20 years, but the substrates used for model evaluation have so far not included pretreated softwood. In the present study, data from lab-scale batch SSF of SO2-impregnated, steam-pretreated spruce chips were used to evaluate a model found in the literature. The model, which was somewhat modified, consists of a number of nonlinear, coupled ordinary differential equations, which were solved numerically. Some parameter values were fitted to data by use of least-squares minimization. A difficulty in parameter estimation was the lack of cellobiose measurements, something that was relieved by adding assumptions about parameter relations. The simulated... (More)
Simultaneous saccharification and fermentation (SSF) of wood has been modeled for the past 15-20 years, but the substrates used for model evaluation have so far not included pretreated softwood. In the present study, data from lab-scale batch SSF of SO2-impregnated, steam-pretreated spruce chips were used to evaluate a model found in the literature. The model, which was somewhat modified, consists of a number of nonlinear, coupled ordinary differential equations, which were solved numerically. Some parameter values were fitted to data by use of least-squares minimization. A difficulty in parameter estimation was the lack of cellobiose measurements, something that was relieved by adding assumptions about parameter relations. The simulated concentration profiles agreed well with the measured concentrations of glucose and ethanol. It is therefore concluded that the basic model features apply to softwood SSF. The model predicts rate saturation with respect to enzyme concentration at concentrations above 60 FPU/g cellulose, although this was not observed in the experimental data, which only comprised enzyme concentrations up to 32 FPU/g cellulose. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
simultaneous saccharification and fermentation, softwood, kinetic model
in
Applied Biochemistry and Biotechnology
volume
98
pages
733 - 746
publisher
Humana Press
external identifiers
  • wos:000175257800063
  • scopus:0036234658
ISSN
1559-0291
language
English
LU publication?
yes
id
e4f96691-310f-47d5-8ba7-9b122ddb59d8 (old id 339715)
alternative location
http://www.ingentaconnect.com/content/hum/abab/2002/00000099/F0030001/art00023
date added to LUP
2007-11-08 16:00:54
date last changed
2017-04-30 07:59:53
@article{e4f96691-310f-47d5-8ba7-9b122ddb59d8,
  abstract     = {Simultaneous saccharification and fermentation (SSF) of wood has been modeled for the past 15-20 years, but the substrates used for model evaluation have so far not included pretreated softwood. In the present study, data from lab-scale batch SSF of SO2-impregnated, steam-pretreated spruce chips were used to evaluate a model found in the literature. The model, which was somewhat modified, consists of a number of nonlinear, coupled ordinary differential equations, which were solved numerically. Some parameter values were fitted to data by use of least-squares minimization. A difficulty in parameter estimation was the lack of cellobiose measurements, something that was relieved by adding assumptions about parameter relations. The simulated concentration profiles agreed well with the measured concentrations of glucose and ethanol. It is therefore concluded that the basic model features apply to softwood SSF. The model predicts rate saturation with respect to enzyme concentration at concentrations above 60 FPU/g cellulose, although this was not observed in the experimental data, which only comprised enzyme concentrations up to 32 FPU/g cellulose.},
  author       = {Pettersson, PO and Eklund, R and Zacchi, Guido},
  issn         = {1559-0291},
  keyword      = {simultaneous saccharification and fermentation,softwood,kinetic model},
  language     = {eng},
  pages        = {733--746},
  publisher    = {Humana Press},
  series       = {Applied Biochemistry and Biotechnology},
  title        = {Modeling simultaneous saccharification and fermentation of softwood},
  volume       = {98},
  year         = {2002},
}