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Proteomic analysis of phytophthora infestans reveals the importance of cell wall proteins in pathogenicity

Resjö, Svante LU ; Brus, Maja; Ali, Ashfaq LU ; Meijer, Harold J. G.; Sandin, Marianne LU ; Govers, Francine; Levander, Fredrik LU ; Grenville-Briggs, Laura J. and Andreasson, Erik (2017) In Molecular and Cellular Proteomics 16(11). p.1958-1971
Abstract

The oomycete Phytophthora infestans is the most harmful pathogen of potato. It causes the disease late blight, which generates increased yearly costs of up to one billion euro in the EU alone and is difficult to control. We have performed a large-scale quantitative proteomics study of six P. infestans life stages with the aim to identify proteins that change in abundance during development, with a focus on preinfectious life stages. Over 10 000 peptides from 2061 proteins were analyzed. We identified several abundance profiles of proteins that were up- or downregulated in different combinations of life stages. One of these profiles contained 59 proteins that were more abundant in germinated cysts and appressoria. A large majority of... (More)

The oomycete Phytophthora infestans is the most harmful pathogen of potato. It causes the disease late blight, which generates increased yearly costs of up to one billion euro in the EU alone and is difficult to control. We have performed a large-scale quantitative proteomics study of six P. infestans life stages with the aim to identify proteins that change in abundance during development, with a focus on preinfectious life stages. Over 10 000 peptides from 2061 proteins were analyzed. We identified several abundance profiles of proteins that were up- or downregulated in different combinations of life stages. One of these profiles contained 59 proteins that were more abundant in germinated cysts and appressoria. A large majority of these proteins were not previously recognized as being appressorial proteins or involved in the infection process. Among those are proteins with putative roles in transport, amino acid metabolism, pathogenicity (including one RXLR effector) and cell wall structure modification. We analyzed the expression of the genes encoding nine of these proteins using RT-qPCR and found an increase in transcript levels during disease progression, in agreement with the hypothesis that these proteins are important in early infection. Among the nine proteins was a group involved in cell wall structure modification and adhesion, including three closely related, uncharacterized proteins encoded by PITG-01131, PITG-01132, and PITG-16135, here denoted Piacwp1-3. Transient silencing of these genes resulted in reduced severity of infection, indicating that these proteins are important for pathogenicity. Our results contribute to further insight into P. infestans biology, and indicate processes that might be relevant for the pathogen while preparing for host cell penetration and during infection. The mass spectrometry data have been deposited to ProteomeXchange via the PRIDE partner repository with the data set identifier PXD002446.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Molecular and Cellular Proteomics
volume
16
issue
11
pages
14 pages
publisher
American Society for Biochemistry and Molecular Biology
external identifiers
  • scopus:85032643073
ISSN
1535-9476
DOI
10.1074/mcp.M116.065656
language
English
LU publication?
yes
id
33e826ae-3ce6-4c4f-b1f5-988ef8e27703
date added to LUP
2017-11-10 10:32:45
date last changed
2018-01-07 12:25:35
@article{33e826ae-3ce6-4c4f-b1f5-988ef8e27703,
  abstract     = {<p>The oomycete Phytophthora infestans is the most harmful pathogen of potato. It causes the disease late blight, which generates increased yearly costs of up to one billion euro in the EU alone and is difficult to control. We have performed a large-scale quantitative proteomics study of six P. infestans life stages with the aim to identify proteins that change in abundance during development, with a focus on preinfectious life stages. Over 10 000 peptides from 2061 proteins were analyzed. We identified several abundance profiles of proteins that were up- or downregulated in different combinations of life stages. One of these profiles contained 59 proteins that were more abundant in germinated cysts and appressoria. A large majority of these proteins were not previously recognized as being appressorial proteins or involved in the infection process. Among those are proteins with putative roles in transport, amino acid metabolism, pathogenicity (including one RXLR effector) and cell wall structure modification. We analyzed the expression of the genes encoding nine of these proteins using RT-qPCR and found an increase in transcript levels during disease progression, in agreement with the hypothesis that these proteins are important in early infection. Among the nine proteins was a group involved in cell wall structure modification and adhesion, including three closely related, uncharacterized proteins encoded by PITG-01131, PITG-01132, and PITG-16135, here denoted Piacwp1-3. Transient silencing of these genes resulted in reduced severity of infection, indicating that these proteins are important for pathogenicity. Our results contribute to further insight into P. infestans biology, and indicate processes that might be relevant for the pathogen while preparing for host cell penetration and during infection. The mass spectrometry data have been deposited to ProteomeXchange via the PRIDE partner repository with the data set identifier PXD002446.</p>},
  author       = {Resjö, Svante and Brus, Maja and Ali, Ashfaq and Meijer, Harold J. G. and Sandin, Marianne and Govers, Francine and Levander, Fredrik and Grenville-Briggs, Laura J. and Andreasson, Erik},
  issn         = {1535-9476},
  language     = {eng},
  month        = {11},
  number       = {11},
  pages        = {1958--1971},
  publisher    = {American Society for Biochemistry and Molecular Biology},
  series       = {Molecular and Cellular Proteomics},
  title        = {Proteomic analysis of phytophthora infestans reveals the importance of cell wall proteins in pathogenicity},
  url          = {http://dx.doi.org/10.1074/mcp.M116.065656},
  volume       = {16},
  year         = {2017},
}