Membrane-based continuous fermentation with cell recycling for propionic acid production from glycerol by Acidipropionibacterium acidipropionici
(2023) In Microbial Cell Factories 22.- Abstract
Background: Microbial production of propionic acid (PA) from renewable resources is limited by the slow growth of the producer bacteria and product-mediated inhibition. The present study evaluates high cell density continuous PA fermentation from glycerol (Gly) using Acidipropionibacterium acidipropionici DSM 4900 in a membrane-based cell recycling system. A ceramic tubular membrane filter of 0.22 μm pore size was used as the filtering device for cell recycling. The continuous fermentations were run sequentially at dilution rates of 0.05 and 0.025 1/h using varying glycerol concentrations and two different yeast extract concentrations.
Results: PA volumetric productivity of 0.98 g/L.h with a product yield of 0.38... (More)
Background: Microbial production of propionic acid (PA) from renewable resources is limited by the slow growth of the producer bacteria and product-mediated inhibition. The present study evaluates high cell density continuous PA fermentation from glycerol (Gly) using Acidipropionibacterium acidipropionici DSM 4900 in a membrane-based cell recycling system. A ceramic tubular membrane filter of 0.22 μm pore size was used as the filtering device for cell recycling. The continuous fermentations were run sequentially at dilution rates of 0.05 and 0.025 1/h using varying glycerol concentrations and two different yeast extract concentrations.
Results: PA volumetric productivity of 0.98 g/L.h with a product yield of 0.38 gPA/gGly was obtained with 51.40 g/L glycerol at a yeast extract concentration of 10 g/L. Increasing the glycerol and yeast extract concentrations to 64.50 g/L and 20 g/L, respectively, increased in PA productivity, product yield, and concentration to 1.82 g/L.h, 0.79 gPA/gGly, and 38.37 g/L, respectively. However, lowering the dilution rate to 0.025 1/h reduced the production efficiency. The cell density increased from 5.80 to 91.83 gCDW/L throughout the operation, which lasted for a period of 5 months. A tolerant variant of A. acidipropoinici exhibiting growth at a PA concentration of 20 g/L was isolated at the end of the experiment.
Conclusions: Applying the current approach for PA fermentation can overcome several limitations for process industrialization.
(Less)
- author
- Cavero-Olguin, Victor Hugo LU ; Dishisha, Tarek LU and Hatti-Kaul, Rajni LU
- organization
- publishing date
- 2023-12
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Biorefinery, Fermentation, Glycerol, Propionibacteria, Propionic acid
- in
- Microbial Cell Factories
- volume
- 22
- article number
- 43
- pages
- 10 pages
- publisher
- BioMed Central (BMC)
- external identifiers
-
- scopus:85149596658
- pmid:36870992
- ISSN
- 1475-2859
- DOI
- 10.1186/s12934-023-02049-7
- language
- English
- LU publication?
- yes
- additional info
- Publisher Copyright: © 2023, The Author(s).
- id
- 3403f047-502b-47ce-a395-e1490128afc7
- date added to LUP
- 2023-03-30 09:27:22
- date last changed
- 2024-04-18 19:45:30
@article{3403f047-502b-47ce-a395-e1490128afc7,
abstract = {{<p><b>Background</b>: Microbial production of propionic acid (PA) from renewable resources is limited by the slow growth of the producer bacteria and product-mediated inhibition. The present study evaluates high cell density continuous PA fermentation from glycerol (Gly) using Acidipropionibacterium acidipropionici DSM 4900 in a membrane-based cell recycling system. A ceramic tubular membrane filter of 0.22 μm pore size was used as the filtering device for cell recycling. The continuous fermentations were run sequentially at dilution rates of 0.05 and 0.025 1/h using varying glycerol concentrations and two different yeast extract concentrations.</p><p><b>Results</b>: PA volumetric productivity of 0.98 g/L.h with a product yield of 0.38 g<sub>PA</sub>/g<sub>Gly</sub> was obtained with 51.40 g/L glycerol at a yeast extract concentration of 10 g/L. Increasing the glycerol and yeast extract concentrations to 64.50 g/L and 20 g/L, respectively, increased in PA productivity, product yield, and concentration to 1.82 g/L.h, 0.79 g<sub>PA</sub>/g<sub>Gly</sub>, and 38.37 g/L, respectively. However, lowering the dilution rate to 0.025 1/h reduced the production efficiency. The cell density increased from 5.80 to 91.83 g<sub>CDW</sub>/L throughout the operation, which lasted for a period of 5 months. A tolerant variant of A. acidipropoinici exhibiting growth at a PA concentration of 20 g/L was isolated at the end of the experiment.</p><p><b>Conclusions</b>: Applying the current approach for PA fermentation can overcome several limitations for process industrialization.</p>}},
author = {{Cavero-Olguin, Victor Hugo and Dishisha, Tarek and Hatti-Kaul, Rajni}},
issn = {{1475-2859}},
keywords = {{Biorefinery; Fermentation; Glycerol; Propionibacteria; Propionic acid}},
language = {{eng}},
publisher = {{BioMed Central (BMC)}},
series = {{Microbial Cell Factories}},
title = {{Membrane-based continuous fermentation with cell recycling for propionic acid production from glycerol by <i>Acidipropionibacterium</i> <i>acidipropionici</i>}},
url = {{http://dx.doi.org/10.1186/s12934-023-02049-7}},
doi = {{10.1186/s12934-023-02049-7}},
volume = {{22}},
year = {{2023}},
}