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Redox hydrogel-based bienzyme microelectrodes for amperometric monitoring of L-glutamate

Mikeladze, E; Schulte, A; Mosbach, M; Blochl, A; Csöregi, Elisabeth LU ; Solomonia, R and Schumann, W (2002) In Electroanalysis 14(6). p.393-399
Abstract
Fabrication and characterization of amperometric bienzyme L-glutamate sensitive microelectrodes are the prerequisite for monitoring changes Of L-glutamate concentration at glutamate-secreting cell cultures. The design of the glutamate microelectrodes is based on incorporating L-glutamate oxidase and horseradish peroxidase into a redox-hydrogel containing PVI19-dmeOs as the redox mediator and immobilizing this system onto the surface of platinum microdisk electrodes using, a dip-coating procedure. For amperometric measurements Of L-glutamate, these redox hydrogel-based bienzyme microelectrodes can be operated at low working potentials (-50 mV vs. Ag/AgCl) decreasing the influence of electroactive interferants possibly present in biological... (More)
Fabrication and characterization of amperometric bienzyme L-glutamate sensitive microelectrodes are the prerequisite for monitoring changes Of L-glutamate concentration at glutamate-secreting cell cultures. The design of the glutamate microelectrodes is based on incorporating L-glutamate oxidase and horseradish peroxidase into a redox-hydrogel containing PVI19-dmeOs as the redox mediator and immobilizing this system onto the surface of platinum microdisk electrodes using, a dip-coating procedure. For amperometric measurements Of L-glutamate, these redox hydrogel-based bienzyme microelectrodes can be operated at low working potentials (-50 mV vs. Ag/AgCl) decreasing the influence of electroactive interferants possibly present in biological samples. The L-glutamate microsensors are characterized by a good operation stability and sensitivity (0.038+/-0.005 mAM(-1)), a low detection limit (0.5 muM in a conventional amperometric set-up and 0.03 muM in a Faraday cage, defined as three times the signal-to-noise ratio), a linear range up to 50 muM and a response time of about 35 s. The glutamate biosensors have been applied for the direct measurement Of L-glutamate release (upon chemical stimulation) from a population of immortalized hippocampal neurons (HN10 cells) demonstrating the possibility to amperometrically monitor in-Situ L-glutamate secretion from these cells. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
hippocampal neurons, glutamate secretion, microelectrode, glutamate, amperometric biosensor
in
Electroanalysis
volume
14
issue
6
pages
393 - 399
publisher
John Wiley & Sons
external identifiers
  • wos:000174832900001
  • scopus:0036107897
ISSN
1040-0397
DOI
10.1002/1521-4109(200203)14:6<393::AID-ELAN393>3.0.CO;2-P
language
English
LU publication?
yes
id
b8f8b93b-2b01-4b06-89c5-291f76a2a522 (old id 340844)
date added to LUP
2007-11-02 11:54:11
date last changed
2017-01-01 07:24:43
@article{b8f8b93b-2b01-4b06-89c5-291f76a2a522,
  abstract     = {Fabrication and characterization of amperometric bienzyme L-glutamate sensitive microelectrodes are the prerequisite for monitoring changes Of L-glutamate concentration at glutamate-secreting cell cultures. The design of the glutamate microelectrodes is based on incorporating L-glutamate oxidase and horseradish peroxidase into a redox-hydrogel containing PVI19-dmeOs as the redox mediator and immobilizing this system onto the surface of platinum microdisk electrodes using, a dip-coating procedure. For amperometric measurements Of L-glutamate, these redox hydrogel-based bienzyme microelectrodes can be operated at low working potentials (-50 mV vs. Ag/AgCl) decreasing the influence of electroactive interferants possibly present in biological samples. The L-glutamate microsensors are characterized by a good operation stability and sensitivity (0.038+/-0.005 mAM(-1)), a low detection limit (0.5 muM in a conventional amperometric set-up and 0.03 muM in a Faraday cage, defined as three times the signal-to-noise ratio), a linear range up to 50 muM and a response time of about 35 s. The glutamate biosensors have been applied for the direct measurement Of L-glutamate release (upon chemical stimulation) from a population of immortalized hippocampal neurons (HN10 cells) demonstrating the possibility to amperometrically monitor in-Situ L-glutamate secretion from these cells.},
  author       = {Mikeladze, E and Schulte, A and Mosbach, M and Blochl, A and Csöregi, Elisabeth and Solomonia, R and Schumann, W},
  issn         = {1040-0397},
  keyword      = {hippocampal neurons,glutamate secretion,microelectrode,glutamate,amperometric biosensor},
  language     = {eng},
  number       = {6},
  pages        = {393--399},
  publisher    = {John Wiley & Sons},
  series       = {Electroanalysis},
  title        = {Redox hydrogel-based bienzyme microelectrodes for amperometric monitoring of L-glutamate},
  url          = {http://dx.doi.org/10.1002/1521-4109(200203)14:6<393::AID-ELAN393>3.0.CO;2-P},
  volume       = {14},
  year         = {2002},
}