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Polymorphism in the Calpain 10 gene influences glucose metabolism in human fat cells

Hoffstedt, J; Ryden, M; Lofgren, P; Orho-Melander, Marju LU ; Groop, Leif LU and Arner, P (2002) In Diabetologia 45(2). p.276-282
Abstract
Aims/hypothesis. A common G to A polymorphism (UCSNP-43) in the Calpain 10 gene was recently found to be associated with Type 11 (non-insulin-dependent) diabetes mellitus and variations in post-absorptive and insulin stimulated glucose metabolism in vivo. We aimed to study the influence of Calpain 10 polymorphism on insulin action in fat cells. Methods. Calpain 10 polymorphism (UCSNP-19, -43 or -63) were set in relation to lipolysis and lipogenesis in isolated subcutaneous adipocytes of 46 apparently healthy non-obese subjects. Results. For UCSNP-43 the GIG genotype had twofold higher basal and insulin stimulated rates as compared with AA/AG genotypes. However, there was no genotype effect on basal or insulin inhibited lipolysis rates in... (More)
Aims/hypothesis. A common G to A polymorphism (UCSNP-43) in the Calpain 10 gene was recently found to be associated with Type 11 (non-insulin-dependent) diabetes mellitus and variations in post-absorptive and insulin stimulated glucose metabolism in vivo. We aimed to study the influence of Calpain 10 polymorphism on insulin action in fat cells. Methods. Calpain 10 polymorphism (UCSNP-19, -43 or -63) were set in relation to lipolysis and lipogenesis in isolated subcutaneous adipocytes of 46 apparently healthy non-obese subjects. Results. For UCSNP-43 the GIG genotype had twofold higher basal and insulin stimulated rates as compared with AA/AG genotypes. However, there was no genotype effect on basal or insulin inhibited lipolysis rates in fat cells. The protein amount of GLUT 4 in adipocytes was not influenced by the polymorphism. Fat cells expressed mRNA for the Calpain 10 gene at a relatively high concentration, about 4 amol/mug RNA, which is similar to that of uncoupling protein-2. Neither a UCSNP-19 nor a UCSNP-63 polymorphism in the Calpain 10 gene was found to be associated with basal or insulin-induced adipocyte lipolysis and lipogenesis. None of the polymorphisms influenced body mass index or fasting plasma concentrations of insulin and glucose in 693 non-obese healthy subjects. Conclusion/interpretation. The Calpain 10 gene could be involved in the regulation of glucose metabolism but not lipolysis in human fat cells, although it does not involve adipocyte GLUT-4 protein content. It is possible that the Calpain 10 gene predisposes to diabetes by influencing the glucose metabolism. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
insulin, lipogenesis, lipolysis, polymerase chain reaction, mRNA, adipose tissue, GLUT-4
in
Diabetologia
volume
45
issue
2
pages
276 - 282
publisher
Springer Verlag
external identifiers
  • wos:000174701300016
  • pmid:11935160
  • scopus:0036191328
ISSN
1432-0428
DOI
10.1007/s00125-001-0732-2
language
English
LU publication?
yes
id
a724c6ca-d303-48f0-b119-90b31f793262 (old id 341176)
date added to LUP
2007-10-22 09:33:35
date last changed
2017-01-01 04:45:23
@article{a724c6ca-d303-48f0-b119-90b31f793262,
  abstract     = {Aims/hypothesis. A common G to A polymorphism (UCSNP-43) in the Calpain 10 gene was recently found to be associated with Type 11 (non-insulin-dependent) diabetes mellitus and variations in post-absorptive and insulin stimulated glucose metabolism in vivo. We aimed to study the influence of Calpain 10 polymorphism on insulin action in fat cells. Methods. Calpain 10 polymorphism (UCSNP-19, -43 or -63) were set in relation to lipolysis and lipogenesis in isolated subcutaneous adipocytes of 46 apparently healthy non-obese subjects. Results. For UCSNP-43 the GIG genotype had twofold higher basal and insulin stimulated rates as compared with AA/AG genotypes. However, there was no genotype effect on basal or insulin inhibited lipolysis rates in fat cells. The protein amount of GLUT 4 in adipocytes was not influenced by the polymorphism. Fat cells expressed mRNA for the Calpain 10 gene at a relatively high concentration, about 4 amol/mug RNA, which is similar to that of uncoupling protein-2. Neither a UCSNP-19 nor a UCSNP-63 polymorphism in the Calpain 10 gene was found to be associated with basal or insulin-induced adipocyte lipolysis and lipogenesis. None of the polymorphisms influenced body mass index or fasting plasma concentrations of insulin and glucose in 693 non-obese healthy subjects. Conclusion/interpretation. The Calpain 10 gene could be involved in the regulation of glucose metabolism but not lipolysis in human fat cells, although it does not involve adipocyte GLUT-4 protein content. It is possible that the Calpain 10 gene predisposes to diabetes by influencing the glucose metabolism.},
  author       = {Hoffstedt, J and Ryden, M and Lofgren, P and Orho-Melander, Marju and Groop, Leif and Arner, P},
  issn         = {1432-0428},
  keyword      = {insulin,lipogenesis,lipolysis,polymerase chain reaction,mRNA,adipose tissue,GLUT-4},
  language     = {eng},
  number       = {2},
  pages        = {276--282},
  publisher    = {Springer Verlag},
  series       = {Diabetologia},
  title        = {Polymorphism in the Calpain 10 gene influences glucose metabolism in human fat cells},
  url          = {http://dx.doi.org/10.1007/s00125-001-0732-2},
  volume       = {45},
  year         = {2002},
}