Polymorphism in the Calpain 10 gene influences glucose metabolism in human fat cells
(2002) In Diabetologia 45(2). p.276-282- Abstract
- Aims/hypothesis. A common G to A polymorphism (UCSNP-43) in the Calpain 10 gene was recently found to be associated with Type 11 (non-insulin-dependent) diabetes mellitus and variations in post-absorptive and insulin stimulated glucose metabolism in vivo. We aimed to study the influence of Calpain 10 polymorphism on insulin action in fat cells. Methods. Calpain 10 polymorphism (UCSNP-19, -43 or -63) were set in relation to lipolysis and lipogenesis in isolated subcutaneous adipocytes of 46 apparently healthy non-obese subjects. Results. For UCSNP-43 the GIG genotype had twofold higher basal and insulin stimulated rates as compared with AA/AG genotypes. However, there was no genotype effect on basal or insulin inhibited lipolysis rates in... (More)
- Aims/hypothesis. A common G to A polymorphism (UCSNP-43) in the Calpain 10 gene was recently found to be associated with Type 11 (non-insulin-dependent) diabetes mellitus and variations in post-absorptive and insulin stimulated glucose metabolism in vivo. We aimed to study the influence of Calpain 10 polymorphism on insulin action in fat cells. Methods. Calpain 10 polymorphism (UCSNP-19, -43 or -63) were set in relation to lipolysis and lipogenesis in isolated subcutaneous adipocytes of 46 apparently healthy non-obese subjects. Results. For UCSNP-43 the GIG genotype had twofold higher basal and insulin stimulated rates as compared with AA/AG genotypes. However, there was no genotype effect on basal or insulin inhibited lipolysis rates in fat cells. The protein amount of GLUT 4 in adipocytes was not influenced by the polymorphism. Fat cells expressed mRNA for the Calpain 10 gene at a relatively high concentration, about 4 amol/mug RNA, which is similar to that of uncoupling protein-2. Neither a UCSNP-19 nor a UCSNP-63 polymorphism in the Calpain 10 gene was found to be associated with basal or insulin-induced adipocyte lipolysis and lipogenesis. None of the polymorphisms influenced body mass index or fasting plasma concentrations of insulin and glucose in 693 non-obese healthy subjects. Conclusion/interpretation. The Calpain 10 gene could be involved in the regulation of glucose metabolism but not lipolysis in human fat cells, although it does not involve adipocyte GLUT-4 protein content. It is possible that the Calpain 10 gene predisposes to diabetes by influencing the glucose metabolism. (Less)
Please use this url to cite or link to this publication:
http://lup.lub.lu.se/record/341176
- author
- Hoffstedt, J; Ryden, M; Lofgren, P; Orho-Melander, Marju LU ; Groop, Leif LU and Arner, P
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- insulin, lipogenesis, lipolysis, polymerase chain reaction, mRNA, adipose tissue, GLUT-4
- in
- Diabetologia
- volume
- 45
- issue
- 2
- pages
- 276 - 282
- publisher
- Springer Verlag
- external identifiers
-
- wos:000174701300016
- pmid:11935160
- scopus:0036191328
- ISSN
- 1432-0428
- DOI
- 10.1007/s00125-001-0732-2
- language
- English
- LU publication?
- yes
- id
- a724c6ca-d303-48f0-b119-90b31f793262 (old id 341176)
- date added to LUP
- 2007-10-22 09:33:35
- date last changed
- 2018-01-07 05:40:39
@article{a724c6ca-d303-48f0-b119-90b31f793262, abstract = {Aims/hypothesis. A common G to A polymorphism (UCSNP-43) in the Calpain 10 gene was recently found to be associated with Type 11 (non-insulin-dependent) diabetes mellitus and variations in post-absorptive and insulin stimulated glucose metabolism in vivo. We aimed to study the influence of Calpain 10 polymorphism on insulin action in fat cells. Methods. Calpain 10 polymorphism (UCSNP-19, -43 or -63) were set in relation to lipolysis and lipogenesis in isolated subcutaneous adipocytes of 46 apparently healthy non-obese subjects. Results. For UCSNP-43 the GIG genotype had twofold higher basal and insulin stimulated rates as compared with AA/AG genotypes. However, there was no genotype effect on basal or insulin inhibited lipolysis rates in fat cells. The protein amount of GLUT 4 in adipocytes was not influenced by the polymorphism. Fat cells expressed mRNA for the Calpain 10 gene at a relatively high concentration, about 4 amol/mug RNA, which is similar to that of uncoupling protein-2. Neither a UCSNP-19 nor a UCSNP-63 polymorphism in the Calpain 10 gene was found to be associated with basal or insulin-induced adipocyte lipolysis and lipogenesis. None of the polymorphisms influenced body mass index or fasting plasma concentrations of insulin and glucose in 693 non-obese healthy subjects. Conclusion/interpretation. The Calpain 10 gene could be involved in the regulation of glucose metabolism but not lipolysis in human fat cells, although it does not involve adipocyte GLUT-4 protein content. It is possible that the Calpain 10 gene predisposes to diabetes by influencing the glucose metabolism.}, author = {Hoffstedt, J and Ryden, M and Lofgren, P and Orho-Melander, Marju and Groop, Leif and Arner, P}, issn = {1432-0428}, keyword = {insulin,lipogenesis,lipolysis,polymerase chain reaction,mRNA,adipose tissue,GLUT-4}, language = {eng}, number = {2}, pages = {276--282}, publisher = {Springer Verlag}, series = {Diabetologia}, title = {Polymorphism in the Calpain 10 gene influences glucose metabolism in human fat cells}, url = {http://dx.doi.org/10.1007/s00125-001-0732-2}, volume = {45}, year = {2002}, }