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Gold-coated capillary based 2,4-dichlorophenoxyacetic acid chemi-lumincscent assays: possibilities towards multianalysis

Ramanathan, Kumaran LU ; Dzgoev, Anatoly LU ; Svitel, Juraj LU ; Rees Jönsson, Birgitta LU and Danielsson, Bengt LU (2002) Multianalyte Biosensing Devices Workshop In Biosensors and Bioelectronics 17(4). p.283-288
Abstract
The application of gold-coated glass capillaries for the design of a sensitive chemiluminescent immunoassay for 2,4-dichlorophenoxyacetic acid (2,4-D) is reported. The gold coating on the glass capillaries has been partially characterized and its effect on enhancing the signal intensity has been measured. A simple photo-multiplier tube-based photon detector is used for this purpose. At least three times improvement in the signal intensity is observed compared to uncoated glass capillaries, with a consequent improvement in the sensitivity of detection. Using such gold-coated glass capillaries, 2,4-D in the range 10(-9) to 10(-13) mol/l is detectable at a precision of +/-15% (CV%) and a limit of detection of 10(-15) mol/l is achievable. The... (More)
The application of gold-coated glass capillaries for the design of a sensitive chemiluminescent immunoassay for 2,4-dichlorophenoxyacetic acid (2,4-D) is reported. The gold coating on the glass capillaries has been partially characterized and its effect on enhancing the signal intensity has been measured. A simple photo-multiplier tube-based photon detector is used for this purpose. At least three times improvement in the signal intensity is observed compared to uncoated glass capillaries, with a consequent improvement in the sensitivity of detection. Using such gold-coated glass capillaries, 2,4-D in the range 10(-9) to 10(-13) mol/l is detectable at a precision of +/-15% (CV%) and a limit of detection of 10(-15) mol/l is achievable. The possibility of using such gold-coated capillaries with a portable multianalytical set-up for field studies is also demonstrated. (Less)
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author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
pesticides, detection limit, portable multianalytical set-up, precision, capillaries, Au-coated glass capillaries, Au, acid, sensitivity, photo-multiplier tube-based photon detector, signal intensity, glass capillaries, Au coating, sensitive chemiluminescent immunoassay, design, 4-dichlorophenoxyacetic acid chemiluminescent assays, Au-coated capillary based 2, multianalysis, assays
in
Biosensors and Bioelectronics
volume
17
issue
4
pages
283 - 288
publisher
Elsevier
conference name
Multianalyte Biosensing Devices Workshop
external identifiers
  • pmid:11849924
  • wos:000174693300005
  • scopus:0036179551
ISSN
0956-5663
DOI
10.1016/S0956-5663(01)00296-2
language
English
LU publication?
yes
id
2b675f1e-2e77-46a2-9499-47f35722897d (old id 341241)
date added to LUP
2007-11-28 14:23:56
date last changed
2017-01-01 06:40:04
@inproceedings{2b675f1e-2e77-46a2-9499-47f35722897d,
  abstract     = {The application of gold-coated glass capillaries for the design of a sensitive chemiluminescent immunoassay for 2,4-dichlorophenoxyacetic acid (2,4-D) is reported. The gold coating on the glass capillaries has been partially characterized and its effect on enhancing the signal intensity has been measured. A simple photo-multiplier tube-based photon detector is used for this purpose. At least three times improvement in the signal intensity is observed compared to uncoated glass capillaries, with a consequent improvement in the sensitivity of detection. Using such gold-coated glass capillaries, 2,4-D in the range 10(-9) to 10(-13) mol/l is detectable at a precision of +/-15% (CV%) and a limit of detection of 10(-15) mol/l is achievable. The possibility of using such gold-coated capillaries with a portable multianalytical set-up for field studies is also demonstrated.},
  author       = {Ramanathan, Kumaran and Dzgoev, Anatoly and Svitel, Juraj and Rees Jönsson, Birgitta and Danielsson, Bengt},
  booktitle    = {Biosensors and Bioelectronics},
  issn         = {0956-5663},
  keyword      = {pesticides,detection limit,portable multianalytical set-up,precision,capillaries,Au-coated glass capillaries,Au,acid,sensitivity,photo-multiplier tube-based photon detector,signal intensity,glass capillaries,Au coating,sensitive chemiluminescent immunoassay,design,4-dichlorophenoxyacetic acid chemiluminescent assays,Au-coated capillary based 2,multianalysis,assays},
  language     = {eng},
  number       = {4},
  pages        = {283--288},
  publisher    = {Elsevier},
  title        = {Gold-coated capillary based 2,4-dichlorophenoxyacetic acid chemi-lumincscent assays: possibilities towards multianalysis},
  url          = {http://dx.doi.org/10.1016/S0956-5663(01)00296-2},
  volume       = {17},
  year         = {2002},
}