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Germinal center B cells constitute a predominant physiological source of IL-4: Implication for Th2 development in vivo

Johansson-Lindbom, Bengt and Borrebaeck, Carl LU (2002) In Journal of Immunology 168(7). p.3165-3172
Abstract
Protective immunity depends upon the capability of the immune system to properly adapt the response to the nature of an infectious agent. CD4+ Th cells are implicated in this orchestration by secreting a polarized pattern of cytokines. Although Th2 development in animal models and in human cells in vitro to a large extent depends on IL-4, the nature of the cells that provide the initial IL-4 in vivo is still elusive. In this report, we describe the anatomical localization as well as the identity of IL-4-producing cells in human tonsil, a representative secondary lymphoid organ. We demonstrate that IL-4 production is a normal and intrinsic feature of germinal center (GC) B cells. We also show that expression of IL-4 is highly confined to... (More)
Protective immunity depends upon the capability of the immune system to properly adapt the response to the nature of an infectious agent. CD4+ Th cells are implicated in this orchestration by secreting a polarized pattern of cytokines. Although Th2 development in animal models and in human cells in vitro to a large extent depends on IL-4, the nature of the cells that provide the initial IL-4 in vivo is still elusive. In this report, we describe the anatomical localization as well as the identity of IL-4-producing cells in human tonsil, a representative secondary lymphoid organ. We demonstrate that IL-4 production is a normal and intrinsic feature of germinal center (GC) B cells. We also show that expression of IL-4 is highly confined to the GCs, in which the B cells constitute the prevalent cellular source. Furthermore, immunofluorescence analysis of colon mucosa reveals a strikingly similar pattern of IL-4-expressing cells compared with tonsils, demonstrating that IL-4 production from GC B cells is not a unique feature of the upper respiratory tract. Our results show that GCs provide the most appropriate microenvironment for IL-4-dependent Th2 polarization in vivo and imply a critical role for GC B cells in this differentiation process. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Immunology
volume
168
issue
7
pages
3165 - 3172
publisher
American Association of Immunologists
external identifiers
  • pmid:11907068
  • wos:000174566400008
  • scopus:0036533540
ISSN
1550-6606
language
English
LU publication?
yes
id
2870a11b-92ee-42f0-8549-27f7c933f2e8 (old id 341839)
date added to LUP
2007-10-31 13:10:11
date last changed
2017-10-01 04:49:15
@article{2870a11b-92ee-42f0-8549-27f7c933f2e8,
  abstract     = {Protective immunity depends upon the capability of the immune system to properly adapt the response to the nature of an infectious agent. CD4+ Th cells are implicated in this orchestration by secreting a polarized pattern of cytokines. Although Th2 development in animal models and in human cells in vitro to a large extent depends on IL-4, the nature of the cells that provide the initial IL-4 in vivo is still elusive. In this report, we describe the anatomical localization as well as the identity of IL-4-producing cells in human tonsil, a representative secondary lymphoid organ. We demonstrate that IL-4 production is a normal and intrinsic feature of germinal center (GC) B cells. We also show that expression of IL-4 is highly confined to the GCs, in which the B cells constitute the prevalent cellular source. Furthermore, immunofluorescence analysis of colon mucosa reveals a strikingly similar pattern of IL-4-expressing cells compared with tonsils, demonstrating that IL-4 production from GC B cells is not a unique feature of the upper respiratory tract. Our results show that GCs provide the most appropriate microenvironment for IL-4-dependent Th2 polarization in vivo and imply a critical role for GC B cells in this differentiation process.},
  author       = {Johansson-Lindbom, Bengt and Borrebaeck, Carl},
  issn         = {1550-6606},
  language     = {eng},
  number       = {7},
  pages        = {3165--3172},
  publisher    = {American Association of Immunologists},
  series       = {Journal of Immunology},
  title        = {Germinal center B cells constitute a predominant physiological source of IL-4: Implication for Th2 development in vivo},
  volume       = {168},
  year         = {2002},
}