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Comparative analysis of the mouse and human genes (Matn2 and MATN2) for matrilin-2, a filament-forming protein widely distributed in extracellular matrices

Mates, L; Korpos, E; Deak, F; Liu, ZQ; Beier, DR; Aszodi, Attila LU and Kiss, I (2002) In Matrix Biology 21(2). p.163-174
Abstract
We previously identified matrilin-2 (MATN2), the largest member of the novel family of matrilins. These filament-forming adapter proteins expressed in a distinct, but partially overlapping, pattern in all tissues were implicated in the organization of the extracellular matrix. Matrilin-2 functions in a great variety of tissues. Here, we present the genomic organization of the highly conserved mouse and human MATN2 loci, which cover > 100 kb and 167.167 kb genomic regions, respectively, and are composed of 19 exons. RT-PCR analysis revealed that alternative transcripts with identical protein coding regions are transcribed from two promoters in both species. The upstream, housekeeping type promoter is functional in all tissues and cell... (More)
We previously identified matrilin-2 (MATN2), the largest member of the novel family of matrilins. These filament-forming adapter proteins expressed in a distinct, but partially overlapping, pattern in all tissues were implicated in the organization of the extracellular matrix. Matrilin-2 functions in a great variety of tissues. Here, we present the genomic organization of the highly conserved mouse and human MATN2 loci, which cover > 100 kb and 167.167 kb genomic regions, respectively, and are composed of 19 exons. RT-PCR analysis revealed that alternative transcripts with identical protein coding regions are transcribed from two promoters in both species. The upstream, housekeeping type promoter is functional in all tissues and cell types tested. The activity of the downstream, TATA-like promoter preceded with putative motifs for the homeobox transcription factor PRRX2 is restricted to embryonic fibroblasts and certain cell lines. The oligomerization module is split by an U12-type AT-AC intron found in conserved position in all four matrilin genes. We assigned Matn2 to mouse chromosome 15, linked to Trhr and Sntb1 in a region synthenic to human chromosome 8q22-24. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
PRRX2 motifs, alternative promoters, introns, AT-AC, genomic organization, extracellular matrix, adapter protein
in
Matrix Biology
volume
21
issue
2
pages
163 - 174
publisher
Elsevier
external identifiers
  • wos:000174426100005
  • scopus:0036168619
ISSN
1569-1802
DOI
10.1016/S0945-053X(01)00194-9
language
English
LU publication?
yes
id
5c7568ee-7915-4752-994d-e78d700aa483 (old id 341984)
date added to LUP
2007-11-02 11:27:26
date last changed
2017-09-10 04:25:33
@article{5c7568ee-7915-4752-994d-e78d700aa483,
  abstract     = {We previously identified matrilin-2 (MATN2), the largest member of the novel family of matrilins. These filament-forming adapter proteins expressed in a distinct, but partially overlapping, pattern in all tissues were implicated in the organization of the extracellular matrix. Matrilin-2 functions in a great variety of tissues. Here, we present the genomic organization of the highly conserved mouse and human MATN2 loci, which cover > 100 kb and 167.167 kb genomic regions, respectively, and are composed of 19 exons. RT-PCR analysis revealed that alternative transcripts with identical protein coding regions are transcribed from two promoters in both species. The upstream, housekeeping type promoter is functional in all tissues and cell types tested. The activity of the downstream, TATA-like promoter preceded with putative motifs for the homeobox transcription factor PRRX2 is restricted to embryonic fibroblasts and certain cell lines. The oligomerization module is split by an U12-type AT-AC intron found in conserved position in all four matrilin genes. We assigned Matn2 to mouse chromosome 15, linked to Trhr and Sntb1 in a region synthenic to human chromosome 8q22-24.},
  author       = {Mates, L and Korpos, E and Deak, F and Liu, ZQ and Beier, DR and Aszodi, Attila and Kiss, I},
  issn         = {1569-1802},
  keyword      = {PRRX2 motifs,alternative promoters,introns,AT-AC,genomic organization,extracellular matrix,adapter protein},
  language     = {eng},
  number       = {2},
  pages        = {163--174},
  publisher    = {Elsevier},
  series       = {Matrix Biology},
  title        = {Comparative analysis of the mouse and human genes (Matn2 and MATN2) for matrilin-2, a filament-forming protein widely distributed in extracellular matrices},
  url          = {http://dx.doi.org/10.1016/S0945-053X(01)00194-9},
  volume       = {21},
  year         = {2002},
}