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Characterization of specific IgE response in vitro against protein and drug allergens using atopic and normal donors

Åkesson, A; Ingvarsson, Sigurdur LU ; Karlsson, Fredrik LU ; Leyva, L; Blanca, M; Cuerden, SA; Smith, JA; Coleman, JW and Borrebaeck, Carl LU (2002) In Allergy 57(3). p.193-200
Abstract
Background: As the incidence of allergy to different compounds increases in society, the need to understand and characterize specific IgE responses becomes F. obvious. Different cell culture systems have been evaluated for their ability to support such IgE secretion. Methods: One system employed human peripheral lymphocytes (PBL) from normal donors stimulated with anti-CD3 activated T cells with or without the presence of allergens like benzylpenicillin (BP) and Phlenum pratense (PP). Secretion of IgE was analyzed in ELISA and compared to the IgG response to the nonallergenic antigen tetanus toxoid (TT). Another system employed stimulation of T and B cells with a heterotope, consisting of a T helper cell epitope derived from TT, and a B... (More)
Background: As the incidence of allergy to different compounds increases in society, the need to understand and characterize specific IgE responses becomes F. obvious. Different cell culture systems have been evaluated for their ability to support such IgE secretion. Methods: One system employed human peripheral lymphocytes (PBL) from normal donors stimulated with anti-CD3 activated T cells with or without the presence of allergens like benzylpenicillin (BP) and Phlenum pratense (PP). Secretion of IgE was analyzed in ELISA and compared to the IgG response to the nonallergenic antigen tetanus toxoid (TT). Another system employed stimulation of T and B cells with a heterotope, consisting of a T helper cell epitope derived from TT, and a B cell allergen epitope derived from BP. The specific IgE secretion was compared, using lymphocytes from nor-Mal as well as BP-allergic donors. Results: Anti-CD3 stimulated T cells supported BP-specific IgE secretion in Six of 11 normal donors. This response was inhibited in four donors and enhanced in two donors by the addition of the BP-allergen to the culture. In contrast, addition of the protein allergen (PP) or antigen (TT) to the same culture system inhibited both IgE and IgG synthesis in all experiments. C-ells from the majority (10/16) of the BP-allergic donors failed to produce BP-specific IgE in vitro, when cultured in the presence of allergen. Conclusions: An allergen specific immune response is readily generated in vitro. The differential response against benzylpenicillin between different donor categories most probably reflects the level of pre-exposure to this allergen in vivo. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
in vitro, IgE, grasspollen, allergenicity, benzylpenicillin
in
Allergy
volume
57
issue
3
pages
193 - 200
publisher
Wiley-Blackwell
external identifiers
  • wos:000174527100003
  • pmid:11906332
  • scopus:0036199753
ISSN
1398-9995
DOI
10.1034/j.1398-9995.2002.1o3321.x
language
English
LU publication?
yes
id
67506274-4662-4208-92f0-f325fd2d7159 (old id 342141)
date added to LUP
2007-08-06 14:01:03
date last changed
2017-01-01 06:53:18
@article{67506274-4662-4208-92f0-f325fd2d7159,
  abstract     = {Background: As the incidence of allergy to different compounds increases in society, the need to understand and characterize specific IgE responses becomes F. obvious. Different cell culture systems have been evaluated for their ability to support such IgE secretion. Methods: One system employed human peripheral lymphocytes (PBL) from normal donors stimulated with anti-CD3 activated T cells with or without the presence of allergens like benzylpenicillin (BP) and Phlenum pratense (PP). Secretion of IgE was analyzed in ELISA and compared to the IgG response to the nonallergenic antigen tetanus toxoid (TT). Another system employed stimulation of T and B cells with a heterotope, consisting of a T helper cell epitope derived from TT, and a B cell allergen epitope derived from BP. The specific IgE secretion was compared, using lymphocytes from nor-Mal as well as BP-allergic donors. Results: Anti-CD3 stimulated T cells supported BP-specific IgE secretion in Six of 11 normal donors. This response was inhibited in four donors and enhanced in two donors by the addition of the BP-allergen to the culture. In contrast, addition of the protein allergen (PP) or antigen (TT) to the same culture system inhibited both IgE and IgG synthesis in all experiments. C-ells from the majority (10/16) of the BP-allergic donors failed to produce BP-specific IgE in vitro, when cultured in the presence of allergen. Conclusions: An allergen specific immune response is readily generated in vitro. The differential response against benzylpenicillin between different donor categories most probably reflects the level of pre-exposure to this allergen in vivo.},
  author       = {Åkesson, A and Ingvarsson, Sigurdur and Karlsson, Fredrik and Leyva, L and Blanca, M and Cuerden, SA and Smith, JA and Coleman, JW and Borrebaeck, Carl},
  issn         = {1398-9995},
  keyword      = {in vitro,IgE,grasspollen,allergenicity,benzylpenicillin},
  language     = {eng},
  number       = {3},
  pages        = {193--200},
  publisher    = {Wiley-Blackwell},
  series       = {Allergy},
  title        = {Characterization of specific IgE response in vitro against protein and drug allergens using atopic and normal donors},
  url          = {http://dx.doi.org/10.1034/j.1398-9995.2002.1o3321.x},
  volume       = {57},
  year         = {2002},
}