Protocol for 3D direct reprogramming of human glia into dopaminergic neurons with gene expression and immunocytochemistry validation
(2025) In STAR Protocols 6(4).- Abstract
Direct reprogramming of somatic cells into neurons offers a promising strategy for studying neurological disorders and developing cell-based therapies. This protocol describes the 3D direct reprogramming of human glial progenitor cells into dopaminergic neurons. It includes spheroid formation, lentiviral transduction, neuronal induction using doxycycline, and validation via gene expression and immunocytochemistry. This versatile approach enables efficient reprogramming of human cells into dopaminergic neurons. For complete details on the use and execution of this protocol, please refer to Giacomoni et al.1
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3424abaa-7776-4c74-bd26-24b5d2247cfd
- author
- Giacomoni, Jessica
LU
; Laurin, Kerstin
LU
; Parmar, Malin LU
and Habekost, Mette LU
- organization
- publishing date
- 2025
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Cell Biology, Cell culture, Cell Differentiation, Gene Expression, Genomics, Molecular Biology, Neuroscience, RNA-seq, Stem Cells
- in
- STAR Protocols
- volume
- 6
- issue
- 4
- article number
- 104088
- publisher
- Cell Press
- external identifiers
-
- pmid:40946308
- scopus:105015492064
- ISSN
- 2666-1667
- DOI
- 10.1016/j.xpro.2025.104088
- language
- English
- LU publication?
- yes
- id
- 3424abaa-7776-4c74-bd26-24b5d2247cfd
- date added to LUP
- 2025-10-02 10:14:32
- date last changed
- 2025-10-02 10:14:58
@article{3424abaa-7776-4c74-bd26-24b5d2247cfd, abstract = {{<p>Direct reprogramming of somatic cells into neurons offers a promising strategy for studying neurological disorders and developing cell-based therapies. This protocol describes the 3D direct reprogramming of human glial progenitor cells into dopaminergic neurons. It includes spheroid formation, lentiviral transduction, neuronal induction using doxycycline, and validation via gene expression and immunocytochemistry. This versatile approach enables efficient reprogramming of human cells into dopaminergic neurons. For complete details on the use and execution of this protocol, please refer to Giacomoni et al.<sup>1</sup></p>}}, author = {{Giacomoni, Jessica and Laurin, Kerstin and Parmar, Malin and Habekost, Mette}}, issn = {{2666-1667}}, keywords = {{Cell Biology; Cell culture; Cell Differentiation; Gene Expression; Genomics; Molecular Biology; Neuroscience; RNA-seq; Stem Cells}}, language = {{eng}}, number = {{4}}, publisher = {{Cell Press}}, series = {{STAR Protocols}}, title = {{Protocol for 3D direct reprogramming of human glia into dopaminergic neurons with gene expression and immunocytochemistry validation}}, url = {{http://dx.doi.org/10.1016/j.xpro.2025.104088}}, doi = {{10.1016/j.xpro.2025.104088}}, volume = {{6}}, year = {{2025}}, }