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Protocol for 3D direct reprogramming of human glia into dopaminergic neurons with gene expression and immunocytochemistry validation

Giacomoni, Jessica LU ; Laurin, Kerstin LU orcid ; Parmar, Malin LU orcid and Habekost, Mette LU orcid (2025) In STAR Protocols 6(4).
Abstract

Direct reprogramming of somatic cells into neurons offers a promising strategy for studying neurological disorders and developing cell-based therapies. This protocol describes the 3D direct reprogramming of human glial progenitor cells into dopaminergic neurons. It includes spheroid formation, lentiviral transduction, neuronal induction using doxycycline, and validation via gene expression and immunocytochemistry. This versatile approach enables efficient reprogramming of human cells into dopaminergic neurons. For complete details on the use and execution of this protocol, please refer to Giacomoni et al.1

Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cell Biology, Cell culture, Cell Differentiation, Gene Expression, Genomics, Molecular Biology, Neuroscience, RNA-seq, Stem Cells
in
STAR Protocols
volume
6
issue
4
article number
104088
publisher
Cell Press
external identifiers
  • scopus:105015492064
  • pmid:40946308
ISSN
2666-1667
DOI
10.1016/j.xpro.2025.104088
language
English
LU publication?
yes
id
3424abaa-7776-4c74-bd26-24b5d2247cfd
date added to LUP
2025-10-02 10:14:32
date last changed
2025-10-16 12:00:31
@article{3424abaa-7776-4c74-bd26-24b5d2247cfd,
  abstract     = {{<p>Direct reprogramming of somatic cells into neurons offers a promising strategy for studying neurological disorders and developing cell-based therapies. This protocol describes the 3D direct reprogramming of human glial progenitor cells into dopaminergic neurons. It includes spheroid formation, lentiviral transduction, neuronal induction using doxycycline, and validation via gene expression and immunocytochemistry. This versatile approach enables efficient reprogramming of human cells into dopaminergic neurons. For complete details on the use and execution of this protocol, please refer to Giacomoni et al.<sup>1</sup></p>}},
  author       = {{Giacomoni, Jessica and Laurin, Kerstin and Parmar, Malin and Habekost, Mette}},
  issn         = {{2666-1667}},
  keywords     = {{Cell Biology; Cell culture; Cell Differentiation; Gene Expression; Genomics; Molecular Biology; Neuroscience; RNA-seq; Stem Cells}},
  language     = {{eng}},
  number       = {{4}},
  publisher    = {{Cell Press}},
  series       = {{STAR Protocols}},
  title        = {{Protocol for 3D direct reprogramming of human glia into dopaminergic neurons with gene expression and immunocytochemistry validation}},
  url          = {{http://dx.doi.org/10.1016/j.xpro.2025.104088}},
  doi          = {{10.1016/j.xpro.2025.104088}},
  volume       = {{6}},
  year         = {{2025}},
}