Characterization of macromolecular solutions by a combined static and dynamic light scattering technique
(2002) In International Journal of Thermophysics 23(1). p.161-173- Abstract
- The combination of quasi-elastic light scattering (LS) with integrated scattered intensity measurements in the same sample has been applied to study polymer and polymer-protein aqueous solutions. The molecular weight, the radius of gyration, and the second virial coefficient for thermosensitive polymer [poly(N-isopropylacrylamid)] solutions before and after precipitation transition have been obtained using Zimm plot calculations. The precipitation curve (intensity versus temperature dependence) for polymer solutions has been experimentally obtained using the light scattering setup. For the first time the static and dynamic LS properties of aqueous solutions of antibody-poly(methacrylic acid) and antibody-poly(acrylic acid) conjugates and... (More)
- The combination of quasi-elastic light scattering (LS) with integrated scattered intensity measurements in the same sample has been applied to study polymer and polymer-protein aqueous solutions. The molecular weight, the radius of gyration, and the second virial coefficient for thermosensitive polymer [poly(N-isopropylacrylamid)] solutions before and after precipitation transition have been obtained using Zimm plot calculations. The precipitation curve (intensity versus temperature dependence) for polymer solutions has been experimentally obtained using the light scattering setup. For the first time the static and dynamic LS properties of aqueous solutions of antibody-poly(methacrylic acid) and antibody-poly(acrylic acid) conjugates and solutions of their components [antibody, poly(methacrylic acid), and poly(acrylic acid)] at different pH values have been measured. In both cases the parallel comparison of the characteristic size variations allowed us to represent novel structural features of scattered particles (macromolecules, associates, aggregates, conjugates, colloidal particles) in studied systems. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/342574
- author
- Kazakov, S V ; Galaev, Igor LU and Mattiasson, Bo LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- light, cooperative interactions, conjugate, aggregation, association, protein, polymer, precipitation, scattering
- in
- International Journal of Thermophysics
- volume
- 23
- issue
- 1
- pages
- 161 - 173
- publisher
- Springer
- external identifiers
-
- wos:000174190800012
- scopus:0041866431
- ISSN
- 0195-928X
- DOI
- 10.1023/A:1013957127723
- language
- English
- LU publication?
- yes
- id
- 06985a97-773f-4962-94d2-35eb41b541bc (old id 342574)
- date added to LUP
- 2016-04-01 12:03:15
- date last changed
- 2022-04-13 05:21:30
@article{06985a97-773f-4962-94d2-35eb41b541bc,
abstract = {{The combination of quasi-elastic light scattering (LS) with integrated scattered intensity measurements in the same sample has been applied to study polymer and polymer-protein aqueous solutions. The molecular weight, the radius of gyration, and the second virial coefficient for thermosensitive polymer [poly(N-isopropylacrylamid)] solutions before and after precipitation transition have been obtained using Zimm plot calculations. The precipitation curve (intensity versus temperature dependence) for polymer solutions has been experimentally obtained using the light scattering setup. For the first time the static and dynamic LS properties of aqueous solutions of antibody-poly(methacrylic acid) and antibody-poly(acrylic acid) conjugates and solutions of their components [antibody, poly(methacrylic acid), and poly(acrylic acid)] at different pH values have been measured. In both cases the parallel comparison of the characteristic size variations allowed us to represent novel structural features of scattered particles (macromolecules, associates, aggregates, conjugates, colloidal particles) in studied systems.}},
author = {{Kazakov, S V and Galaev, Igor and Mattiasson, Bo}},
issn = {{0195-928X}},
keywords = {{light; cooperative interactions; conjugate; aggregation; association; protein; polymer; precipitation; scattering}},
language = {{eng}},
number = {{1}},
pages = {{161--173}},
publisher = {{Springer}},
series = {{International Journal of Thermophysics}},
title = {{Characterization of macromolecular solutions by a combined static and dynamic light scattering technique}},
url = {{http://dx.doi.org/10.1023/A:1013957127723}},
doi = {{10.1023/A:1013957127723}},
volume = {{23}},
year = {{2002}},
}