Advanced

Characterization of macromolecular solutions by a combined static and dynamic light scattering technique

Kazakov, S V; Galaev, Igor LU and Mattiasson, Bo LU (2002) In International Journal of Thermophysics 23(1). p.161-173
Abstract
The combination of quasi-elastic light scattering (LS) with integrated scattered intensity measurements in the same sample has been applied to study polymer and polymer-protein aqueous solutions. The molecular weight, the radius of gyration, and the second virial coefficient for thermosensitive polymer [poly(N-isopropylacrylamid)] solutions before and after precipitation transition have been obtained using Zimm plot calculations. The precipitation curve (intensity versus temperature dependence) for polymer solutions has been experimentally obtained using the light scattering setup. For the first time the static and dynamic LS properties of aqueous solutions of antibody-poly(methacrylic acid) and antibody-poly(acrylic acid) conjugates and... (More)
The combination of quasi-elastic light scattering (LS) with integrated scattered intensity measurements in the same sample has been applied to study polymer and polymer-protein aqueous solutions. The molecular weight, the radius of gyration, and the second virial coefficient for thermosensitive polymer [poly(N-isopropylacrylamid)] solutions before and after precipitation transition have been obtained using Zimm plot calculations. The precipitation curve (intensity versus temperature dependence) for polymer solutions has been experimentally obtained using the light scattering setup. For the first time the static and dynamic LS properties of aqueous solutions of antibody-poly(methacrylic acid) and antibody-poly(acrylic acid) conjugates and solutions of their components [antibody, poly(methacrylic acid), and poly(acrylic acid)] at different pH values have been measured. In both cases the parallel comparison of the characteristic size variations allowed us to represent novel structural features of scattered particles (macromolecules, associates, aggregates, conjugates, colloidal particles) in studied systems. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
light, cooperative interactions, conjugate, aggregation, association, protein, polymer, precipitation, scattering
in
International Journal of Thermophysics
volume
23
issue
1
pages
161 - 173
publisher
Kluwer
external identifiers
  • wos:000174190800012
  • scopus:0041866431
ISSN
0195-928X
DOI
10.1023/A:1013957127723
language
English
LU publication?
yes
id
06985a97-773f-4962-94d2-35eb41b541bc (old id 342574)
date added to LUP
2007-11-07 13:45:24
date last changed
2017-12-10 03:46:51
@article{06985a97-773f-4962-94d2-35eb41b541bc,
  abstract     = {The combination of quasi-elastic light scattering (LS) with integrated scattered intensity measurements in the same sample has been applied to study polymer and polymer-protein aqueous solutions. The molecular weight, the radius of gyration, and the second virial coefficient for thermosensitive polymer [poly(N-isopropylacrylamid)] solutions before and after precipitation transition have been obtained using Zimm plot calculations. The precipitation curve (intensity versus temperature dependence) for polymer solutions has been experimentally obtained using the light scattering setup. For the first time the static and dynamic LS properties of aqueous solutions of antibody-poly(methacrylic acid) and antibody-poly(acrylic acid) conjugates and solutions of their components [antibody, poly(methacrylic acid), and poly(acrylic acid)] at different pH values have been measured. In both cases the parallel comparison of the characteristic size variations allowed us to represent novel structural features of scattered particles (macromolecules, associates, aggregates, conjugates, colloidal particles) in studied systems.},
  author       = {Kazakov, S V and Galaev, Igor and Mattiasson, Bo},
  issn         = {0195-928X},
  keyword      = {light,cooperative interactions,conjugate,aggregation,association,protein,polymer,precipitation,scattering},
  language     = {eng},
  number       = {1},
  pages        = {161--173},
  publisher    = {Kluwer},
  series       = {International Journal of Thermophysics},
  title        = {Characterization of macromolecular solutions by a combined static and dynamic light scattering technique},
  url          = {http://dx.doi.org/10.1023/A:1013957127723},
  volume       = {23},
  year         = {2002},
}