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Four or more species of Cladosporium sympatrically colonize Phragmites australis

Wirsel, SGR; Runge-Frobose, C; Ahrén, Dag LU ; Kemen, E; Oliver, RP and Mendgen, KW (2002) In Fungal Genetics and Biology 35(2). p.99-113
Abstract
A collection of Cladosporium has been recovered from common reed growing at Lake Constance (Germany). High-resolution cryo-scanning electron microscopy revealed that Cladosporium isolates from reed are diverse. Morphologically, we distinguished three species, viz. C. herbarum, C. oxysporum, and Cladosporium sp. Internal transcribed spacer (ITS) sequence analysis supported these results and, moreover, separated the most common species, C. oxysporum, into two subclades. Two additional phylogenies were generated to gain support for this finding. The first, differentiating fungi by their capacities to metabolize different carbon sources, showed correlation with morphology. The second, based on actin gene sequences, showed the same overall... (More)
A collection of Cladosporium has been recovered from common reed growing at Lake Constance (Germany). High-resolution cryo-scanning electron microscopy revealed that Cladosporium isolates from reed are diverse. Morphologically, we distinguished three species, viz. C. herbarum, C. oxysporum, and Cladosporium sp. Internal transcribed spacer (ITS) sequence analysis supported these results and, moreover, separated the most common species, C. oxysporum, into two subclades. Two additional phylogenies were generated to gain support for this finding. The first, differentiating fungi by their capacities to metabolize different carbon sources, showed correlation with morphology. The second, based on actin gene sequences, showed the same overall topology as that of the ITS tree, but resulted in a higher resolution indicating the existence of four or more species of Cladosporium on reed. A nested PCR assay targeting variable sequences within actin introns indicated that these four species sympatrically colonize reed. There was no evidence for mutual exclusion on or within the host or specialization for host habitats or organs. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
actin, Phragmites australis, reed, Mycosphaerella, Cladosporium, Septoria, BIOLOG, ITS
in
Fungal Genetics and Biology
volume
35
issue
2
pages
99 - 113
publisher
Elsevier
external identifiers
  • pmid:11848674
  • wos:000174207800002
  • scopus:0036351967
ISSN
1087-1845
DOI
10.1006/fgbi.2001.1314
language
English
LU publication?
yes
id
6ca4b1e3-f530-4c90-98a4-0a8569dadd7d (old id 342596)
date added to LUP
2007-11-16 14:15:18
date last changed
2017-11-05 04:29:53
@article{6ca4b1e3-f530-4c90-98a4-0a8569dadd7d,
  abstract     = {A collection of Cladosporium has been recovered from common reed growing at Lake Constance (Germany). High-resolution cryo-scanning electron microscopy revealed that Cladosporium isolates from reed are diverse. Morphologically, we distinguished three species, viz. C. herbarum, C. oxysporum, and Cladosporium sp. Internal transcribed spacer (ITS) sequence analysis supported these results and, moreover, separated the most common species, C. oxysporum, into two subclades. Two additional phylogenies were generated to gain support for this finding. The first, differentiating fungi by their capacities to metabolize different carbon sources, showed correlation with morphology. The second, based on actin gene sequences, showed the same overall topology as that of the ITS tree, but resulted in a higher resolution indicating the existence of four or more species of Cladosporium on reed. A nested PCR assay targeting variable sequences within actin introns indicated that these four species sympatrically colonize reed. There was no evidence for mutual exclusion on or within the host or specialization for host habitats or organs.},
  author       = {Wirsel, SGR and Runge-Frobose, C and Ahrén, Dag and Kemen, E and Oliver, RP and Mendgen, KW},
  issn         = {1087-1845},
  keyword      = {actin,Phragmites australis,reed,Mycosphaerella,Cladosporium,Septoria,BIOLOG,ITS},
  language     = {eng},
  number       = {2},
  pages        = {99--113},
  publisher    = {Elsevier},
  series       = {Fungal Genetics and Biology},
  title        = {Four or more species of Cladosporium sympatrically colonize Phragmites australis},
  url          = {http://dx.doi.org/10.1006/fgbi.2001.1314},
  volume       = {35},
  year         = {2002},
}