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Engineered bacteria based biosensors for monitoring bioavailable heavy metals

Leth, Suzanne LU ; Maltoni, Susanna; Simkus, Remigijus; Mattiasson, Bo LU ; Corbisier, Philippe; Klimant, Ingo; Wolfbeis, OS and Csöregi, Elisabeth LU (2002) In Electroanalysis 14(1). p.35-42
Abstract
This work presents an integrated analytical system based on immobilized engineered microorganisms and bioluminescence measurements for monitoring of bioavailable heavy metal ions (Cu being chosen as a model ion). A strain of microorganisms from Alcaligenes eutrophus (AE1239) was genetically engineered by inserting a luxCDABE operon from Vibrio fischeri under the control of a copper-induced promoter. As a result, copper ions induce bioluminescence, which is proportional to the concentration of the triggering ions, representing the basis of the design of the hereby described heavy metal biosensor. Microorganisms grown in two different media (Luria Broth and a modified mineral reconstitution medium/RM) were optimized and characterized in... (More)
This work presents an integrated analytical system based on immobilized engineered microorganisms and bioluminescence measurements for monitoring of bioavailable heavy metal ions (Cu being chosen as a model ion). A strain of microorganisms from Alcaligenes eutrophus (AE1239) was genetically engineered by inserting a luxCDABE operon from Vibrio fischeri under the control of a copper-induced promoter. As a result, copper ions induce bioluminescence, which is proportional to the concentration of the triggering ions, representing the basis of the design of the hereby described heavy metal biosensor. Microorganisms grown in two different media (Luria Broth and a modified mineral reconstitution medium/RM) were optimized and characterized in solution with regard to the influence of (growth media and cell density in order to obtain optimal bioluminescent signals. Next, the microorganisms were immobilized in polymer matrices, compatible with fiber optics and were characterized with regard to sensitivity, selectivity, detection limit and storage stability. The lowest detection limit (1 muM) was achieved with microorganisms cultivated from glycerol stock solutions in the RM media and immobilized in a calcium alginate matrix. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
biosensor, genetically engineered Alcaligenes eutrophus, bioluminescence, optical fibers, heavy metal ions
in
Electroanalysis
volume
14
issue
1
pages
35 - 42
publisher
John Wiley & Sons
external identifiers
  • wos:000173607000005
  • scopus:0036162796
ISSN
1040-0397
DOI
10.1002/1521-4109(200201)14:1<35::AID-ELAN35>3.0.CO;2-W
language
English
LU publication?
yes
id
0342343c-d95f-40f4-b142-e8f0223005ba (old id 343900)
date added to LUP
2007-11-14 13:34:33
date last changed
2017-07-23 04:39:46
@article{0342343c-d95f-40f4-b142-e8f0223005ba,
  abstract     = {This work presents an integrated analytical system based on immobilized engineered microorganisms and bioluminescence measurements for monitoring of bioavailable heavy metal ions (Cu being chosen as a model ion). A strain of microorganisms from Alcaligenes eutrophus (AE1239) was genetically engineered by inserting a luxCDABE operon from Vibrio fischeri under the control of a copper-induced promoter. As a result, copper ions induce bioluminescence, which is proportional to the concentration of the triggering ions, representing the basis of the design of the hereby described heavy metal biosensor. Microorganisms grown in two different media (Luria Broth and a modified mineral reconstitution medium/RM) were optimized and characterized in solution with regard to the influence of (growth media and cell density in order to obtain optimal bioluminescent signals. Next, the microorganisms were immobilized in polymer matrices, compatible with fiber optics and were characterized with regard to sensitivity, selectivity, detection limit and storage stability. The lowest detection limit (1 muM) was achieved with microorganisms cultivated from glycerol stock solutions in the RM media and immobilized in a calcium alginate matrix.},
  author       = {Leth, Suzanne and Maltoni, Susanna and Simkus, Remigijus and Mattiasson, Bo and Corbisier, Philippe and Klimant, Ingo and Wolfbeis, OS and Csöregi, Elisabeth},
  issn         = {1040-0397},
  keyword      = {biosensor,genetically engineered Alcaligenes eutrophus,bioluminescence,optical fibers,heavy metal ions},
  language     = {eng},
  number       = {1},
  pages        = {35--42},
  publisher    = {John Wiley & Sons},
  series       = {Electroanalysis},
  title        = {Engineered bacteria based biosensors for monitoring bioavailable heavy metals},
  url          = {http://dx.doi.org/10.1002/1521-4109(200201)14:1<35::AID-ELAN35>3.0.CO;2-W},
  volume       = {14},
  year         = {2002},
}