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Direct bioelectrocatalysis at carbon electrodes modified with quinohemoprotein alcohol dehydrogenase from Gluconobacter sp. 33

Razumiene, J ; Niculescu, M ; Ramanavicius, A ; Laurinavicius, V and Csöregi, Elisabeth LU (2002) In Electroanalysis 14(1). p.43-49
Abstract
A newly isolated, purified, and characterized PQQ-dependent alcohol dehydrogenase (a bacterial membrane-bound protein) was recently found to display a surprisingly large linear range and high selectivity towards ethanol when integrated into a conducting polymer network on a platinum electrode. These findings motivated us to study the enzyme when simply immobilized onto carbonaceous surfaces in order to establish its characteristics and suitability for sensor development, the sensor design being based on a direct-electron transfer pathway. Graphite rods and screen-printed electrodes were modified in two different ways, and were operated both in FIA and batch mode. The obtained biosensor characteristics were highly dependent on the sensor... (More)
A newly isolated, purified, and characterized PQQ-dependent alcohol dehydrogenase (a bacterial membrane-bound protein) was recently found to display a surprisingly large linear range and high selectivity towards ethanol when integrated into a conducting polymer network on a platinum electrode. These findings motivated us to study the enzyme when simply immobilized onto carbonaceous surfaces in order to establish its characteristics and suitability for sensor development, the sensor design being based on a direct-electron transfer pathway. Graphite rods and screen-printed electrodes were modified in two different ways, and were operated both in FIA and batch mode. The obtained biosensor characteristics were highly dependent on the sensor architecture, the highest sensitivity (179 mA M-1 cm(-2)) and lowest detection limit (1 muM) being obtained for screen-printed electrodes used in a batch mode. A mechanism of the observed direct electron transfer between the enzyme's active center and the electrode is proposed. (Less)
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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
screen-printed electrode, bioelectrocatalysis, PQQ-dependent alcohol dehydrogenase
in
Electroanalysis
volume
14
issue
1
pages
43 - 49
publisher
John Wiley and Sons
external identifiers
  • wos:000173607000006
  • scopus:0036162632
ISSN
1040-0397
DOI
10.1002/1521-4109(200201)14:1<43::AID-ELAN43>3.0.CO;2-5
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Department of Chemistry (011001220), Analytical Chemistry (S/LTH) (011001004)
id
d8990ea5-dcc5-4859-bb42-1fbb8d91f6e9 (old id 343909)
date added to LUP
2016-04-01 15:29:24
date last changed
2021-01-06 02:00:29
@article{d8990ea5-dcc5-4859-bb42-1fbb8d91f6e9,
  abstract     = {A newly isolated, purified, and characterized PQQ-dependent alcohol dehydrogenase (a bacterial membrane-bound protein) was recently found to display a surprisingly large linear range and high selectivity towards ethanol when integrated into a conducting polymer network on a platinum electrode. These findings motivated us to study the enzyme when simply immobilized onto carbonaceous surfaces in order to establish its characteristics and suitability for sensor development, the sensor design being based on a direct-electron transfer pathway. Graphite rods and screen-printed electrodes were modified in two different ways, and were operated both in FIA and batch mode. The obtained biosensor characteristics were highly dependent on the sensor architecture, the highest sensitivity (179 mA M-1 cm(-2)) and lowest detection limit (1 muM) being obtained for screen-printed electrodes used in a batch mode. A mechanism of the observed direct electron transfer between the enzyme's active center and the electrode is proposed.},
  author       = {Razumiene, J and Niculescu, M and Ramanavicius, A and Laurinavicius, V and Csöregi, Elisabeth},
  issn         = {1040-0397},
  language     = {eng},
  number       = {1},
  pages        = {43--49},
  publisher    = {John Wiley and Sons},
  series       = {Electroanalysis},
  title        = {Direct bioelectrocatalysis at carbon electrodes modified with quinohemoprotein alcohol dehydrogenase from Gluconobacter sp. 33},
  url          = {http://dx.doi.org/10.1002/1521-4109(200201)14:1<43::AID-ELAN43>3.0.CO;2-5},
  doi          = {10.1002/1521-4109(200201)14:1<43::AID-ELAN43>3.0.CO;2-5},
  volume       = {14},
  year         = {2002},
}