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A new cyclodextrin glycosyltransferase from an alkaliphilic Bacillus agaradhaerens isolate: purification and characterisation

Martins, Rita LU and Hatti-Kaul, Rajni LU (2002) In Enzyme and Microbial Technology 30(1). p.116-124
Abstract
A new, cyclodextrin glycosyltransferase (CGTase) from an alkaliphilic B. agaradhaerens strain LS-3C, isolated front an Ethiopian soda lake. was purified up to 43-fold by starch adsorption with a yield of 50%. The enzyme was a monomer with an estimated molecular weight of 110 kDa, representing the largest Bacillus CGTase reported so far. The isoelectric point (pI) of the enzyme was 6.9. The CGTase was stable over a very wide pH range, 5.0-11.4, at 25degreesC and was most active at pH 9.0. The enzyme exhibited an optimum temperature of 55 C and was stable up to 40degreesC for at least 1 h. Thermal stability could be improved in the presence of substrate, CaCl2, and to a lesser extent, by the product. The enzyme activity Was stimulated by... (More)
A new, cyclodextrin glycosyltransferase (CGTase) from an alkaliphilic B. agaradhaerens strain LS-3C, isolated front an Ethiopian soda lake. was purified up to 43-fold by starch adsorption with a yield of 50%. The enzyme was a monomer with an estimated molecular weight of 110 kDa, representing the largest Bacillus CGTase reported so far. The isoelectric point (pI) of the enzyme was 6.9. The CGTase was stable over a very wide pH range, 5.0-11.4, at 25degreesC and was most active at pH 9.0. The enzyme exhibited an optimum temperature of 55 C and was stable up to 40degreesC for at least 1 h. Thermal stability could be improved in the presence of substrate, CaCl2, and to a lesser extent, by the product. The enzyme activity Was stimulated by CaCl2 but was strongly inhibited by CuCl2, FeCl2, HgCl2, Fe(ClO4)(3), and N-bromosuccinimide. The enzyme produced mainly beta-CD (89% of the total cyclodextrin amount) with only alpha-CD as a minor product. The maximal conversion of maltodextrin to cyclodextrins varied between 10-15% depending on substrate concentration, Cyclodextrin formation was reduced with increase in enzyme concentration beyond 5 U/g substrate. and was marginal at 30 U/g suggesting the predominance of competing hydrolytic reactions catalysed by the enzyme. (C) 2002 Elsevier Science Inc. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
characterization, purification, cyclodextrin glycosyltransferase, Bacillus agaradhaerens, cyclodextrins, alkaliphile
in
Enzyme and Microbial Technology
volume
30
issue
1
pages
116 - 124
publisher
Elsevier
external identifiers
  • wos:000173306700016
  • scopus:0037039473
ISSN
0141-0229
DOI
10.1016/S0141-0229(01)00461-6
language
English
LU publication?
yes
id
167edeeb-6f17-42de-aebe-af69e3c1e399 (old id 344497)
date added to LUP
2007-11-19 14:03:23
date last changed
2017-09-17 05:22:57
@article{167edeeb-6f17-42de-aebe-af69e3c1e399,
  abstract     = {A new, cyclodextrin glycosyltransferase (CGTase) from an alkaliphilic B. agaradhaerens strain LS-3C, isolated front an Ethiopian soda lake. was purified up to 43-fold by starch adsorption with a yield of 50%. The enzyme was a monomer with an estimated molecular weight of 110 kDa, representing the largest Bacillus CGTase reported so far. The isoelectric point (pI) of the enzyme was 6.9. The CGTase was stable over a very wide pH range, 5.0-11.4, at 25degreesC and was most active at pH 9.0. The enzyme exhibited an optimum temperature of 55 C and was stable up to 40degreesC for at least 1 h. Thermal stability could be improved in the presence of substrate, CaCl2, and to a lesser extent, by the product. The enzyme activity Was stimulated by CaCl2 but was strongly inhibited by CuCl2, FeCl2, HgCl2, Fe(ClO4)(3), and N-bromosuccinimide. The enzyme produced mainly beta-CD (89% of the total cyclodextrin amount) with only alpha-CD as a minor product. The maximal conversion of maltodextrin to cyclodextrins varied between 10-15% depending on substrate concentration, Cyclodextrin formation was reduced with increase in enzyme concentration beyond 5 U/g substrate. and was marginal at 30 U/g suggesting the predominance of competing hydrolytic reactions catalysed by the enzyme. (C) 2002 Elsevier Science Inc. All rights reserved.},
  author       = {Martins, Rita and Hatti-Kaul, Rajni},
  issn         = {0141-0229},
  keyword      = {characterization,purification,cyclodextrin glycosyltransferase,Bacillus agaradhaerens,cyclodextrins,alkaliphile},
  language     = {eng},
  number       = {1},
  pages        = {116--124},
  publisher    = {Elsevier},
  series       = {Enzyme and Microbial Technology},
  title        = {A new cyclodextrin glycosyltransferase from an alkaliphilic Bacillus agaradhaerens isolate: purification and characterisation},
  url          = {http://dx.doi.org/10.1016/S0141-0229(01)00461-6},
  volume       = {30},
  year         = {2002},
}