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Neoglycoconjugates of mannan with bovine serum albumin and their interaction with lectin concanavalin A

Mislovicova, D; Masarova, J; Svitel, Juraj LU ; Mendichi, R; Soltes, L; Gemeiner, P and Danielsson, Bengt LU (2002) In Bioconjugate Chemistry 13(1). p.136-142
Abstract
Neoglycoconjugates were prepared from mannan isolated from yeast Saccharomyces cerevisiae and activated by periodate oxidation to create aldehyde groups. Various degrees of oxidation introduced 11-28 aldehyde groups per mannan molecule and simultaneously resulted in a molar mass decrease from 46 to 44.5-31 kDa. The activated mannans were subsequently conjugated with bovine serum albumin forming neoglycoconjugates. Some parameters of these mannan-bovine serum albumin conjugates were characterized: saccharide content 25-30% w/w, molar mass within the range 169-246 kDa, and polydispersion (M-w/M-n) from 2.8 to 3.6. The interaction of these conjugates with lectin concanavalin A was studied using three different methods: W quantitative... (More)
Neoglycoconjugates were prepared from mannan isolated from yeast Saccharomyces cerevisiae and activated by periodate oxidation to create aldehyde groups. Various degrees of oxidation introduced 11-28 aldehyde groups per mannan molecule and simultaneously resulted in a molar mass decrease from 46 to 44.5-31 kDa. The activated mannans were subsequently conjugated with bovine serum albumin forming neoglycoconjugates. Some parameters of these mannan-bovine serum albumin conjugates were characterized: saccharide content 25-30% w/w, molar mass within the range 169-246 kDa, and polydispersion (M-w/M-n) from 2.8 to 3.6. The interaction of these conjugates with lectin concanavalin A was studied using three different methods: W quantitative precipitation in solution; (ii) sorption to concanavalin A immobilized on bead cellulose; and (iii) kinetic measurement of the interaction by surface plasmon resonance. Quantitative precipitation assay showed only negligible differences in the precipitation course of original mannan and the corresponding mannan-bovine serum albumin conjugates. Both the sorption method (equilibrium method) and the surface plasmon resonance measurement (kinetic method) demonstrates that the values of dissociation constant K-D of all synthetic neoglycoconjugates were within the range 10(-7)-10(-8) mol.L-1 (close to K-D = 10(-1) mol-L-1 determined by the sorption method for the original mannan). In conclusion, characterization of synthetic neoglycoconjugates confirmed that the method used for their preparation retained the ability of mannan moiety to interact with concanavalin A. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Bioconjugate Chemistry
volume
13
issue
1
pages
136 - 142
publisher
The American Chemical Society
external identifiers
  • wos:000173374300018
  • scopus:0036007602
ISSN
1520-4812
DOI
10.1021/bc015517u
language
English
LU publication?
yes
id
2760e015-1786-4676-a514-3c5f43f0befa (old id 344564)
date added to LUP
2007-11-16 09:34:48
date last changed
2017-08-06 03:36:15
@article{2760e015-1786-4676-a514-3c5f43f0befa,
  abstract     = {Neoglycoconjugates were prepared from mannan isolated from yeast Saccharomyces cerevisiae and activated by periodate oxidation to create aldehyde groups. Various degrees of oxidation introduced 11-28 aldehyde groups per mannan molecule and simultaneously resulted in a molar mass decrease from 46 to 44.5-31 kDa. The activated mannans were subsequently conjugated with bovine serum albumin forming neoglycoconjugates. Some parameters of these mannan-bovine serum albumin conjugates were characterized: saccharide content 25-30% w/w, molar mass within the range 169-246 kDa, and polydispersion (M-w/M-n) from 2.8 to 3.6. The interaction of these conjugates with lectin concanavalin A was studied using three different methods: W quantitative precipitation in solution; (ii) sorption to concanavalin A immobilized on bead cellulose; and (iii) kinetic measurement of the interaction by surface plasmon resonance. Quantitative precipitation assay showed only negligible differences in the precipitation course of original mannan and the corresponding mannan-bovine serum albumin conjugates. Both the sorption method (equilibrium method) and the surface plasmon resonance measurement (kinetic method) demonstrates that the values of dissociation constant K-D of all synthetic neoglycoconjugates were within the range 10(-7)-10(-8) mol.L-1 (close to K-D = 10(-1) mol-L-1 determined by the sorption method for the original mannan). In conclusion, characterization of synthetic neoglycoconjugates confirmed that the method used for their preparation retained the ability of mannan moiety to interact with concanavalin A.},
  author       = {Mislovicova, D and Masarova, J and Svitel, Juraj and Mendichi, R and Soltes, L and Gemeiner, P and Danielsson, Bengt},
  issn         = {1520-4812},
  language     = {eng},
  number       = {1},
  pages        = {136--142},
  publisher    = {The American Chemical Society},
  series       = {Bioconjugate Chemistry},
  title        = {Neoglycoconjugates of mannan with bovine serum albumin and their interaction with lectin concanavalin A},
  url          = {http://dx.doi.org/10.1021/bc015517u},
  volume       = {13},
  year         = {2002},
}