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Preparation of Films of a Highly Aligned Lipid Cubic Phase

Squires, Adam M. ; Hallett, James E. ; Beddoes, Charlotte M. ; Plivelic, Tomás LU and Seddon, Annela M. (2013) In Langmuir 29(6). p.1726-1731
Abstract
We demonstrate a method by which we can produce an oriented film of an inverse bicontinuous cubic phase (QII,D) formed by the lipid monoolein (MO). By starting with the lipid as a disordered precursor (the L3 phase) in the presence of butanediol, we can obtain a film of the QII,D phase

showing a high degree of in-plane orientation by controlled dilution of the sample under shear within a linear flow cell. We demonstrate that the direction of orientation of the film is different from that found in the oriented bulk material that we have reported previously; therefore, we can now reproducibly form QII,D D samples oriented with either the [110] or the [100] axis aligned in the flow direction depending on the method of preparation.... (More)
We demonstrate a method by which we can produce an oriented film of an inverse bicontinuous cubic phase (QII,D) formed by the lipid monoolein (MO). By starting with the lipid as a disordered precursor (the L3 phase) in the presence of butanediol, we can obtain a film of the QII,D phase

showing a high degree of in-plane orientation by controlled dilution of the sample under shear within a linear flow cell. We demonstrate that the direction of orientation of the film is different from that found in the oriented bulk material that we have reported previously; therefore, we can now reproducibly form QII,D D samples oriented with either the [110] or the [100] axis aligned in the flow direction depending on the method of preparation. The deposition of MO as a film, via a moving fluid−air interface that leaves a coating of MO in the L3 phase on the capillary wall, leads to a sample in the [110] orientation. This contrasts with the bulk material that we have previously demonstrated to be oriented in the [100] direction, arising from flow producing an oriented bulk slug of material within the capillary tube. The bulk sample contains significant amounts of residual butanediol, which can be estimated from the lattice parameter of the QII,D phase obtained. The sample orientation and lattice

parameters are determined from synchrotron small-angle X-ray scattering patterns and confirmed by simulations. This has potential applications in the production of template materials and the growth of protein crystals for crystallography as well as deepening our understanding of the mechanisms underlying the behavior of lyotropic liquid-crystal phases. (Less)
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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
lyotropic liquid-crystal phases, SAXS, template materials
in
Langmuir
volume
29
issue
6
pages
1726 - 1731
publisher
The American Chemical Society (ACS)
external identifiers
  • wos:000315018200002
  • scopus:84873657809
  • pmid:23347289
ISSN
0743-7463
DOI
10.1021/la304726m
language
English
LU publication?
yes
id
6998e068-0270-4a21-b8a2-6189caec8ba3 (old id 3450844)
date added to LUP
2016-04-01 10:22:23
date last changed
2020-02-26 01:46:29
@article{6998e068-0270-4a21-b8a2-6189caec8ba3,
  abstract     = {We demonstrate a method by which we can produce an oriented film of an inverse bicontinuous cubic phase (QII,D) formed by the lipid monoolein (MO). By starting with the lipid as a disordered precursor (the L3 phase) in the presence of butanediol, we can obtain a film of the QII,D phase<br/><br>
showing a high degree of in-plane orientation by controlled dilution of the sample under shear within a linear flow cell. We demonstrate that the direction of orientation of the film is different from that found in the oriented bulk material that we have reported previously; therefore, we can now reproducibly form QII,D D samples oriented with either the [110] or the [100] axis aligned in the flow direction depending on the method of preparation. The deposition of MO as a film, via a moving fluid−air interface that leaves a coating of MO in the L3 phase on the capillary wall, leads to a sample in the [110] orientation. This contrasts with the bulk material that we have previously demonstrated to be oriented in the [100] direction, arising from flow producing an oriented bulk slug of material within the capillary tube. The bulk sample contains significant amounts of residual butanediol, which can be estimated from the lattice parameter of the QII,D phase obtained. The sample orientation and lattice<br/><br>
parameters are determined from synchrotron small-angle X-ray scattering patterns and confirmed by simulations. This has potential applications in the production of template materials and the growth of protein crystals for crystallography as well as deepening our understanding of the mechanisms underlying the behavior of lyotropic liquid-crystal phases.},
  author       = {Squires, Adam M. and Hallett, James E. and Beddoes, Charlotte M. and Plivelic, Tomás and Seddon, Annela M.},
  issn         = {0743-7463},
  language     = {eng},
  number       = {6},
  pages        = {1726--1731},
  publisher    = {The American Chemical Society (ACS)},
  series       = {Langmuir},
  title        = {Preparation of Films of a Highly Aligned Lipid Cubic Phase},
  url          = {http://dx.doi.org/10.1021/la304726m},
  doi          = {10.1021/la304726m},
  volume       = {29},
  year         = {2013},
}