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Antibody responses against B-cell epitopes of the hypervariable region 1 of hepatitis C virus in self-limiting and chronic human hepatitis C followed-up using consensus peptides

Isaguliants, MG; Widell, Anders LU ; Zhang, SMM; Sidorchuk, A; Levi, M; Smirnov, VD; Santantonio, T; Diepolder, HM; Pape, GR and Nordenfelt, E (2002) In Journal of Medical Virology 66(2). p.204-217
Abstract
A rare collection of serum samples from patients with hepatitis C virus (HCV) infection followed up from the onset of clinical symptoms was acquired. RNA corresponding to the hypervariable region 1 (HVR1) of E2 protein of HCV isolated from nine patients was reverse-transcribed, amplified, sequenced, and HVR1 amino acid sequences were deduced. These sequences and a selection of HVR1 amino acid sequences of matching HCV genotypes from protein and translated DNA sequence databanks were used to create the HVR1 amino acid consensus. The degenerated peptides mimicking N- and C-termini of the consensus were synthesized. Most (76%) of 17 patients followed up for the period from 1 week to a minimum of 7 months from the onset of acute symptoms... (More)
A rare collection of serum samples from patients with hepatitis C virus (HCV) infection followed up from the onset of clinical symptoms was acquired. RNA corresponding to the hypervariable region 1 (HVR1) of E2 protein of HCV isolated from nine patients was reverse-transcribed, amplified, sequenced, and HVR1 amino acid sequences were deduced. These sequences and a selection of HVR1 amino acid sequences of matching HCV genotypes from protein and translated DNA sequence databanks were used to create the HVR1 amino acid consensus. The degenerated peptides mimicking N- and C-termini of the consensus were synthesized. Most (76%) of 17 patients followed up for the period from 1 week to a minimum of 7 months from the onset of acute symptoms developed antibodies reacting with peptides representing N- and/or C- termini of HVR1. Antibody recognition of the consensus HVR1 peptides indicates that the variability of HVR1 sequence on the protein level is limited with certain conserved structure(s) being untouched. A tendency was observed for a slower development of anti-HVR1 antibody response in patients developing chronic HCV, as compared to those with self-limiting HCV infection. J. Med. Virol. 66.204-217,2002. (C) 2002 Wiley-Liss, Inc. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
chronic, HCVHVR1 amino acid consensus, antibody response kinetics, self-limited, prediction of clearance
in
Journal of Medical Virology
volume
66
issue
2
pages
204 - 217
publisher
John Wiley & Sons
external identifiers
  • pmid:11782929
  • wos:000172929900009
  • scopus:0036137073
ISSN
1096-9071
DOI
10.1002/jmv.2131
language
English
LU publication?
yes
id
d261042b-4fb5-416a-8db8-5c1440f4e238 (old id 347672)
date added to LUP
2007-10-23 10:24:14
date last changed
2017-01-01 05:05:17
@article{d261042b-4fb5-416a-8db8-5c1440f4e238,
  abstract     = {A rare collection of serum samples from patients with hepatitis C virus (HCV) infection followed up from the onset of clinical symptoms was acquired. RNA corresponding to the hypervariable region 1 (HVR1) of E2 protein of HCV isolated from nine patients was reverse-transcribed, amplified, sequenced, and HVR1 amino acid sequences were deduced. These sequences and a selection of HVR1 amino acid sequences of matching HCV genotypes from protein and translated DNA sequence databanks were used to create the HVR1 amino acid consensus. The degenerated peptides mimicking N- and C-termini of the consensus were synthesized. Most (76%) of 17 patients followed up for the period from 1 week to a minimum of 7 months from the onset of acute symptoms developed antibodies reacting with peptides representing N- and/or C- termini of HVR1. Antibody recognition of the consensus HVR1 peptides indicates that the variability of HVR1 sequence on the protein level is limited with certain conserved structure(s) being untouched. A tendency was observed for a slower development of anti-HVR1 antibody response in patients developing chronic HCV, as compared to those with self-limiting HCV infection. J. Med. Virol. 66.204-217,2002. (C) 2002 Wiley-Liss, Inc.},
  author       = {Isaguliants, MG and Widell, Anders and Zhang, SMM and Sidorchuk, A and Levi, M and Smirnov, VD and Santantonio, T and Diepolder, HM and Pape, GR and Nordenfelt, E},
  issn         = {1096-9071},
  keyword      = {chronic,HCVHVR1 amino acid consensus,antibody response kinetics,self-limited,prediction of clearance},
  language     = {eng},
  number       = {2},
  pages        = {204--217},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Medical Virology},
  title        = {Antibody responses against B-cell epitopes of the hypervariable region 1 of hepatitis C virus in self-limiting and chronic human hepatitis C followed-up using consensus peptides},
  url          = {http://dx.doi.org/10.1002/jmv.2131},
  volume       = {66},
  year         = {2002},
}