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Origin of monocytes and macrophages in a committed progenitor

Hettinger, Jan; Richards, David M; Hansson, Jenny LU ; Barra, Melanie M; Joschko, Ann-Cathrin; Krijgsveld, Jeroen and Feuerer, Markus (2013) In Nature Immunology 14(8). p.30-821
Abstract

Monocytes, macrophages and dendritic cells (DCs) are developmentally related regulators of the immune system that share the monocyte-macrophage DC progenitor (MDP) as a common precursor. Unlike differentiation into DCs, the distal pathways for differentiation into monocytes and monocyte-derived macrophages are not fully elucidated. We have now demonstrated the existence of a clonogenic, monocyte- and macrophage-restricted progenitor cell derived from the MDP. This progenitor was a Ly6C(+) proliferating cell present in the bone marrow and spleen that generated the major monocyte subsets and macrophages, but not DCs or neutrophils. By in-depth quantitative proteomics, we characterized changes in the proteome during monocyte... (More)

Monocytes, macrophages and dendritic cells (DCs) are developmentally related regulators of the immune system that share the monocyte-macrophage DC progenitor (MDP) as a common precursor. Unlike differentiation into DCs, the distal pathways for differentiation into monocytes and monocyte-derived macrophages are not fully elucidated. We have now demonstrated the existence of a clonogenic, monocyte- and macrophage-restricted progenitor cell derived from the MDP. This progenitor was a Ly6C(+) proliferating cell present in the bone marrow and spleen that generated the major monocyte subsets and macrophages, but not DCs or neutrophils. By in-depth quantitative proteomics, we characterized changes in the proteome during monocyte differentiation, which provided insight into the molecular principles of developing monocytes, such as their functional maturation. Thus, we found that monocytes and macrophages were renewed independently of DCs from a committed progenitor.

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publication status
published
keywords
Animals, Bone Marrow, Cell Differentiation, Chromatography, Liquid, Female, Male, Mice, Mice, Inbred C57BL, Monocyte-Macrophage Precursor Cells, Proteomics, Specific Pathogen-Free Organisms, Spectrometry, Mass, Electrospray Ionization, Spleen, Tandem Mass Spectrometry
in
Nature Immunology
volume
14
issue
8
pages
10 pages
publisher
Nature Publishing Group
external identifiers
  • scopus:84880838451
ISSN
1529-2908
DOI
10.1038/ni.2638
language
English
LU publication?
no
id
34ec8816-7d49-4639-ad9e-05813057e66c
date added to LUP
2016-08-25 09:47:56
date last changed
2017-07-23 05:17:09
@article{34ec8816-7d49-4639-ad9e-05813057e66c,
  abstract     = {<p>Monocytes, macrophages and dendritic cells (DCs) are developmentally related regulators of the immune system that share the monocyte-macrophage DC progenitor (MDP) as a common precursor. Unlike differentiation into DCs, the distal pathways for differentiation into monocytes and monocyte-derived macrophages are not fully elucidated. We have now demonstrated the existence of a clonogenic, monocyte- and macrophage-restricted progenitor cell derived from the MDP. This progenitor was a Ly6C(+) proliferating cell present in the bone marrow and spleen that generated the major monocyte subsets and macrophages, but not DCs or neutrophils. By in-depth quantitative proteomics, we characterized changes in the proteome during monocyte differentiation, which provided insight into the molecular principles of developing monocytes, such as their functional maturation. Thus, we found that monocytes and macrophages were renewed independently of DCs from a committed progenitor.</p>},
  author       = {Hettinger, Jan and Richards, David M and Hansson, Jenny and Barra, Melanie M and Joschko, Ann-Cathrin and Krijgsveld, Jeroen and Feuerer, Markus},
  issn         = {1529-2908},
  keyword      = {Animals,Bone Marrow,Cell Differentiation,Chromatography, Liquid,Female,Male,Mice,Mice, Inbred C57BL,Monocyte-Macrophage Precursor Cells,Proteomics,Specific Pathogen-Free Organisms,Spectrometry, Mass, Electrospray Ionization,Spleen,Tandem Mass Spectrometry},
  language     = {eng},
  number       = {8},
  pages        = {30--821},
  publisher    = {Nature Publishing Group},
  series       = {Nature Immunology},
  title        = {Origin of monocytes and macrophages in a committed progenitor},
  url          = {http://dx.doi.org/10.1038/ni.2638},
  volume       = {14},
  year         = {2013},
}