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Gene duplications and losses among vertebrate deoxyribonucleoside kinases of the non-TK1 Family

Mutahir, Zeeshan LU ; Christiansen, Louise Slot LU ; Clausen, Anders R. LU ; Berchtold, Martin W. ; Gojkovic, Zoran ; Munch-Petersen, Birgitte LU ; Knecht, Wolfgang LU and Piskur, Jure LU (2016) In Nucleosides, Nucleotides and Nucleic Acids 35(10-12). p.677-690
Abstract

Deoxyribonucleoside kinases (dNKs) salvage deoxyribonucleosides (dNs) and catalyze the rate limiting step of this salvage pathway by converting dNs into corresponding monophosphate forms. These enzymes serve as an excellent model to study duplicated genes and their evolutionary history. So far, among vertebrates only four mammalian dNKs have been studied for their substrate specificity and kinetic properties. However, some vertebrates, such as fish, frogs, and birds, apparently possess a duplicated homolog of deoxycytidine kinase (dCK). In this study, we characterized a family of dCK/deoxyguanosine kinase (dGK)-like enzymes from a frog Xenopus laevis and a bird Gallus gallus. We showed that X. laevis has a duplicated dCK gene and a dGK... (More)

Deoxyribonucleoside kinases (dNKs) salvage deoxyribonucleosides (dNs) and catalyze the rate limiting step of this salvage pathway by converting dNs into corresponding monophosphate forms. These enzymes serve as an excellent model to study duplicated genes and their evolutionary history. So far, among vertebrates only four mammalian dNKs have been studied for their substrate specificity and kinetic properties. However, some vertebrates, such as fish, frogs, and birds, apparently possess a duplicated homolog of deoxycytidine kinase (dCK). In this study, we characterized a family of dCK/deoxyguanosine kinase (dGK)-like enzymes from a frog Xenopus laevis and a bird Gallus gallus. We showed that X. laevis has a duplicated dCK gene and a dGK gene, whereas G. gallus has a duplicated dCK gene but has lost the dGK gene. We cloned, expressed, purified, and subsequently determined the kinetic parameters of the dCK/dGK enzymes encoded by these genes. The two dCK enzymes in G. gallus have broader substrate specificity than their human or X. laevis counterparts. Additionally, the duplicated dCK enzyme in G. gallus might have become mitochondria. Based on our study we postulate that changing and adapting substrate specificities and subcellular localization are likely the drivers behind the evolution of vertebrate dNKs.

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author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
deoxyribonucleosides, evolution, Gallus gallus, Nucleoside salvage pathway, Xenopus laevis
in
Nucleosides, Nucleotides and Nucleic Acids
volume
35
issue
10-12
pages
14 pages
publisher
Taylor & Francis
external identifiers
  • pmid:27906638
  • wos:000389143100022
  • scopus:85000799344
ISSN
1525-7770
DOI
10.1080/15257770.2016.1143557
language
English
LU publication?
yes
id
34eda4ec-6b4e-4531-bc07-39fdb79b76d6
date added to LUP
2016-12-19 06:57:40
date last changed
2025-01-12 17:55:28
@article{34eda4ec-6b4e-4531-bc07-39fdb79b76d6,
  abstract     = {{<p>Deoxyribonucleoside kinases (dNKs) salvage deoxyribonucleosides (dNs) and catalyze the rate limiting step of this salvage pathway by converting dNs into corresponding monophosphate forms. These enzymes serve as an excellent model to study duplicated genes and their evolutionary history. So far, among vertebrates only four mammalian dNKs have been studied for their substrate specificity and kinetic properties. However, some vertebrates, such as fish, frogs, and birds, apparently possess a duplicated homolog of deoxycytidine kinase (dCK). In this study, we characterized a family of dCK/deoxyguanosine kinase (dGK)-like enzymes from a frog Xenopus laevis and a bird Gallus gallus. We showed that X. laevis has a duplicated dCK gene and a dGK gene, whereas G. gallus has a duplicated dCK gene but has lost the dGK gene. We cloned, expressed, purified, and subsequently determined the kinetic parameters of the dCK/dGK enzymes encoded by these genes. The two dCK enzymes in G. gallus have broader substrate specificity than their human or X. laevis counterparts. Additionally, the duplicated dCK enzyme in G. gallus might have become mitochondria. Based on our study we postulate that changing and adapting substrate specificities and subcellular localization are likely the drivers behind the evolution of vertebrate dNKs.</p>}},
  author       = {{Mutahir, Zeeshan and Christiansen, Louise Slot and Clausen, Anders R. and Berchtold, Martin W. and Gojkovic, Zoran and Munch-Petersen, Birgitte and Knecht, Wolfgang and Piskur, Jure}},
  issn         = {{1525-7770}},
  keywords     = {{deoxyribonucleosides; evolution; Gallus gallus; Nucleoside salvage pathway; Xenopus laevis}},
  language     = {{eng}},
  month        = {{12}},
  number       = {{10-12}},
  pages        = {{677--690}},
  publisher    = {{Taylor & Francis}},
  series       = {{Nucleosides, Nucleotides and Nucleic Acids}},
  title        = {{Gene duplications and losses among vertebrate deoxyribonucleoside kinases of the non-TK1 Family}},
  url          = {{http://dx.doi.org/10.1080/15257770.2016.1143557}},
  doi          = {{10.1080/15257770.2016.1143557}},
  volume       = {{35}},
  year         = {{2016}},
}