Advanced

Methodological considerations on the determination of the APC response in plasma

Andersson, N. E. LU ; Lindberg, K.; Johansson, K. LU ; Rosen, S.; Mahlke, K.; Rabe, K F; Zoller, B. LU and Dahlback, B. LU (1995) In Infusionstherapie und Transfusionsmedizin 22(SUPPL. 1). p.80-82
Abstract

The performance of COATEST® APC® Resistance on different coagulation instruments has been further evaluated through analysis of plasma from 100 blood donors on KC 10, ST 4, ACL 300R, Electra 900 and Thrombolyzer. The electromechanical instruments KC-10 and ST 4 showed a lower response to APC than the turbidimetric or photometric instruments with median APC ratios of 2.7 and 2.8 for the two former versus 3.1, 3.4 and 3.5, respectively, for the other three, which is in agreement with earlier initial findings. Similarly, the cut-off value varied between 2.1 and 2.6 for these instruments. The correlation of APC ratios between instruments was strong with r values ranging between 0.71 and 0.93 and, furthermore, none of the six plasmas with... (More)

The performance of COATEST® APC® Resistance on different coagulation instruments has been further evaluated through analysis of plasma from 100 blood donors on KC 10, ST 4, ACL 300R, Electra 900 and Thrombolyzer. The electromechanical instruments KC-10 and ST 4 showed a lower response to APC than the turbidimetric or photometric instruments with median APC ratios of 2.7 and 2.8 for the two former versus 3.1, 3.4 and 3.5, respectively, for the other three, which is in agreement with earlier initial findings. Similarly, the cut-off value varied between 2.1 and 2.6 for these instruments. The correlation of APC ratios between instruments was strong with r values ranging between 0.71 and 0.93 and, furthermore, none of the six plasmas with the lowest APC ratios on the Thrombolyzer ranked higher than 8 on any of the other instruments. Analysis of control plasmas with six consecutive kit batches on ACL and ST4 resulted in APC ratio ranges of 3.3-3.7 and 2.7-3.0 for a normal control and 1.8-2.0 and 1.9-2.0 for an abnormal control on ACL and ST4, respectively, illustrating a high reproducibility between batches. Repeated freezing and thawing of samples is disrecommended since this often resulted in increased APC ratios. In contrast, in spite of up to 40% decrease in FVIII activity upon storage of 10 different plasma samples for 5 h, the effect on the APC ratio was only minor as was also the effect of addition of 1.0 IU/ml of FVIII. In neither case was any sample misclassified. Altogether, the results support the applicability of this kit for measuring the response of plasma to APC.

(Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
activated protein C (APC), APC response in plasma
in
Infusionstherapie und Transfusionsmedizin
volume
22
issue
SUPPL. 1
pages
3 pages
publisher
Karger
external identifiers
  • scopus:0028802186
ISSN
1019-8466
DOI
10.1159/000223197
language
English
LU publication?
yes
id
34fb05cc-dcf7-4db2-83db-73d4b2097db4
date added to LUP
2017-11-02 11:14:19
date last changed
2017-11-29 14:09:35
@article{34fb05cc-dcf7-4db2-83db-73d4b2097db4,
  abstract     = {<p>The performance of COATEST® APC® Resistance on different coagulation instruments has been further evaluated through analysis of plasma from 100 blood donors on KC 10, ST 4, ACL 300R, Electra 900 and Thrombolyzer. The electromechanical instruments KC-10 and ST 4 showed a lower response to APC than the turbidimetric or photometric instruments with median APC ratios of 2.7 and 2.8 for the two former versus 3.1, 3.4 and 3.5, respectively, for the other three, which is in agreement with earlier initial findings. Similarly, the cut-off value varied between 2.1 and 2.6 for these instruments. The correlation of APC ratios between instruments was strong with r values ranging between 0.71 and 0.93 and, furthermore, none of the six plasmas with the lowest APC ratios on the Thrombolyzer ranked higher than 8 on any of the other instruments. Analysis of control plasmas with six consecutive kit batches on ACL and ST4 resulted in APC ratio ranges of 3.3-3.7 and 2.7-3.0 for a normal control and 1.8-2.0 and 1.9-2.0 for an abnormal control on ACL and ST4, respectively, illustrating a high reproducibility between batches. Repeated freezing and thawing of samples is disrecommended since this often resulted in increased APC ratios. In contrast, in spite of up to 40% decrease in FVIII activity upon storage of 10 different plasma samples for 5 h, the effect on the APC ratio was only minor as was also the effect of addition of 1.0 IU/ml of FVIII. In neither case was any sample misclassified. Altogether, the results support the applicability of this kit for measuring the response of plasma to APC.</p>},
  author       = {Andersson, N. E. and Lindberg, K. and Johansson, K. and Rosen, S. and Mahlke, K. and Rabe, K F and Zoller, B. and Dahlback, B.},
  issn         = {1019-8466},
  keyword      = {activated protein C (APC),APC response in plasma},
  language     = {eng},
  number       = {SUPPL. 1},
  pages        = {80--82},
  publisher    = {Karger},
  series       = {Infusionstherapie und Transfusionsmedizin},
  title        = {Methodological considerations on the determination of the APC response in plasma},
  url          = {http://dx.doi.org/10.1159/000223197},
  volume       = {22},
  year         = {1995},
}