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Changes in optical properties of human whole blood in vitro due to slow heating

Nilsson, A. M. K.; Lucassen, G. W.; Verkruysse, W. and van Gemert, M. J. C. (1997) In Photochemistry and Photobiology 65(2). p.366-373
Abstract
Optical properties of human whole blood were investigated in vitro at 633 nm using a double integrating sphere set-up, The blood flow was maintained at a constant rate through a flow cell while continuously heating the blood at 0.2-1.1 degrees C/min from approximately 25 to 55 degrees C in a heat exchanger, A small, but rather abrupt decrease in the scattering asymmetry factor (g-factor) of 1.7 +/- 0.6% and a similar increase in the scattering coefficient of 2.9 +/- 0.6% were observed at approximately 45-46 degrees C yielding an increase in the reduced scattering coefficient of 40 +/- 10%. Furthermore, a continuous, manifest increase in the absorption coefficient was seen with increasing temperature, on average 80 +/- 70% from 25 to 50... (More)
Optical properties of human whole blood were investigated in vitro at 633 nm using a double integrating sphere set-up, The blood flow was maintained at a constant rate through a flow cell while continuously heating the blood at 0.2-1.1 degrees C/min from approximately 25 to 55 degrees C in a heat exchanger, A small, but rather abrupt decrease in the scattering asymmetry factor (g-factor) of 1.7 +/- 0.6% and a similar increase in the scattering coefficient of 2.9 +/- 0.6% were observed at approximately 45-46 degrees C yielding an increase in the reduced scattering coefficient of 40 +/- 10%. Furthermore, a continuous, manifest increase in the absorption coefficient was seen with increasing temperature, on average 80 +/- 70% from 25 to 50 degrees C. The effect of the heating on the blood cells was also studied under a white-light transmission microscope, A sudden change in the shape of the red blood cells, from disc-shaped to spherical, was observed at approximately the same temperature at which the distinct changes in g-factor and scattering coefficient were observed, i.e. at 45-46 degrees C, The results indicate that this shape transformation could explain the sudden change in scattering properties. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Photochemistry and Photobiology
volume
65
issue
2
pages
366 - 373
publisher
American Society for Photobiology
external identifiers
  • scopus:0031080405
ISSN
0031-8655
language
English
LU publication?
no
id
51d835f7-1857-4409-aec1-238d173d6986 (old id 35057)
date added to LUP
2007-07-23 14:48:48
date last changed
2017-01-01 04:45:59
@article{51d835f7-1857-4409-aec1-238d173d6986,
  abstract     = {Optical properties of human whole blood were investigated in vitro at 633 nm using a double integrating sphere set-up, The blood flow was maintained at a constant rate through a flow cell while continuously heating the blood at 0.2-1.1 degrees C/min from approximately 25 to 55 degrees C in a heat exchanger, A small, but rather abrupt decrease in the scattering asymmetry factor (g-factor) of 1.7 +/- 0.6% and a similar increase in the scattering coefficient of 2.9 +/- 0.6% were observed at approximately 45-46 degrees C yielding an increase in the reduced scattering coefficient of 40 +/- 10%. Furthermore, a continuous, manifest increase in the absorption coefficient was seen with increasing temperature, on average 80 +/- 70% from 25 to 50 degrees C. The effect of the heating on the blood cells was also studied under a white-light transmission microscope, A sudden change in the shape of the red blood cells, from disc-shaped to spherical, was observed at approximately the same temperature at which the distinct changes in g-factor and scattering coefficient were observed, i.e. at 45-46 degrees C, The results indicate that this shape transformation could explain the sudden change in scattering properties.},
  author       = {Nilsson, A. M. K. and Lucassen, G. W. and Verkruysse, W. and van Gemert, M. J. C.},
  issn         = {0031-8655},
  language     = {eng},
  number       = {2},
  pages        = {366--373},
  publisher    = {American Society for Photobiology},
  series       = {Photochemistry and Photobiology},
  title        = {Changes in optical properties of human whole blood in vitro due to slow heating},
  volume       = {65},
  year         = {1997},
}