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Universal method for synthesis of artificial gel antibodies by the imprinting approach combined with a unique electrophoresis technique for detection of minute structural differences of proteins, viruses, and cells (bacteria). III: Gel antibodies against cells (bacteria)

Bacskay, Ivett; Takátsy, Anikó; Végvári, Ákos LU ; Elfwing, Andres; Ballagi-Pordány, András; Kilár, Ferenc and Hjertén, Stellan (2006) In Electrophoresis 27(23). p.4682-4687
Abstract
Artificial antibodies in the form of gel granules were synthesized from the monomers acrylamide and N,N’-methylenebisacrylamide by the imprinting method in the presence of Echerichia coli bacteria as template. The electrophoretic migration velocities of the gel antibodies (i) saturated with the antigen (Escherichia coli MRE-600), (ii) freed of the antigen, and (iii) resaturated with bacteria, were determinated by electrophoresis in a rotating narrow-bore tube of 245 mm length and the 2.5 and 9.6 mm inner and outer diameters, respectively. Removal of bacteria from the gel antibodies was made by treatment with enzymes, followed by washing with SDS and buffer. Gel granules becoming charged by adsorption of bacteria move in an electrical... (More)
Artificial antibodies in the form of gel granules were synthesized from the monomers acrylamide and N,N’-methylenebisacrylamide by the imprinting method in the presence of Echerichia coli bacteria as template. The electrophoretic migration velocities of the gel antibodies (i) saturated with the antigen (Escherichia coli MRE-600), (ii) freed of the antigen, and (iii) resaturated with bacteria, were determinated by electrophoresis in a rotating narrow-bore tube of 245 mm length and the 2.5 and 9.6 mm inner and outer diameters, respectively. Removal of bacteria from the gel antibodies was made by treatment with enzymes, followed by washing with SDS and buffer. Gel granules becoming charged by adsorption of bacteria move in an electrical field. We obtained a significant selectivity of gel antibodies for E. coli MRE-600, since the granules did not interact with Lactococcus lactis; and when E. coli BL21 bacteria were added to the gels selective for E. coli MRE-600, a significant difference in the migration rate of the complexes formed with the two strains was observed indicating the ability of differentiation between the two strains. The gel antibodies can be used repeatedly. The new imprinting method for the synthesis of artificial gel antibodies against bioparticles described herein, and the classical electrophoretic analysis technique employed, thus represent – when combined – a new approach to distinguish between different types and strains of bacteria. The application area can certainly be extended to cover other classes of cells. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Artificial antibodies, Bacteria, Escherichia coli, Free-zone electrophoresis, Imprinting
in
Electrophoresis
volume
27
issue
23
pages
4682 - 4687
publisher
John Wiley & Sons
external identifiers
  • scopus:33845581421
ISSN
0173-0835
DOI
10.1002/elps.200600192
language
English
LU publication?
no
id
afa83acd-0345-44e3-9f6f-284851f6d5a9 (old id 3516199)
date added to LUP
2013-02-25 09:13:09
date last changed
2019-05-28 02:28:29
@article{afa83acd-0345-44e3-9f6f-284851f6d5a9,
  abstract     = {Artificial antibodies in the form of gel granules were synthesized from the monomers acrylamide and N,N’-methylenebisacrylamide by the imprinting method in the presence of Echerichia coli bacteria as template. The electrophoretic migration velocities of the gel antibodies (i) saturated with the antigen (Escherichia coli MRE-600), (ii) freed of the antigen, and (iii) resaturated with bacteria, were determinated by electrophoresis in a rotating narrow-bore tube of 245 mm length and the 2.5 and 9.6 mm inner and outer diameters, respectively. Removal of bacteria from the gel antibodies was made by treatment with enzymes, followed by washing with SDS and buffer. Gel granules becoming charged by adsorption of bacteria move in an electrical field. We obtained a significant selectivity of gel antibodies for E. coli MRE-600, since the granules did not interact with Lactococcus lactis; and when E. coli BL21 bacteria were added to the gels selective for E. coli MRE-600, a significant difference in the migration rate of the complexes formed with the two strains was observed indicating the ability of differentiation between the two strains. The gel antibodies can be used repeatedly. The new imprinting method for the synthesis of artificial gel antibodies against bioparticles described herein, and the classical electrophoretic analysis technique employed, thus represent – when combined – a new approach to distinguish between different types and strains of bacteria. The application area can certainly be extended to cover other classes of cells.},
  author       = {Bacskay, Ivett and Takátsy, Anikó and Végvári, Ákos and Elfwing, Andres and Ballagi-Pordány, András and Kilár, Ferenc and Hjertén, Stellan},
  issn         = {0173-0835},
  keyword      = {Artificial antibodies,Bacteria,Escherichia coli,Free-zone electrophoresis,Imprinting},
  language     = {eng},
  number       = {23},
  pages        = {4682--4687},
  publisher    = {John Wiley & Sons},
  series       = {Electrophoresis},
  title        = {Universal method for synthesis of artificial gel antibodies by the imprinting approach combined with a unique electrophoresis technique for detection of minute structural differences of proteins, viruses, and cells (bacteria). III: Gel antibodies against cells (bacteria)},
  url          = {http://dx.doi.org/10.1002/elps.200600192},
  volume       = {27},
  year         = {2006},
}