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Hybrid Microdevice Electrophoretic of Peptides, Proteins, DNA, Viruses and Bacteria in Various Separations Media Using UV-Detection

Végvári, Ákos LU and Hjertén, Stellan (2003) In Electrophoresis 24(21). p.3815-3820
Abstract
Recently we described the design of a hybrid microdevice for micro(nano)electrophoresis and electrochromatography, discussed its advantages and disadvantages compared to conventional microdevices and presented a few applications with lowmolecular-weight samples. In this paper, we demonstrate the broad application range of this device using UV-based analyses of (i) peptides by free-zone electrophoresis and

electrophoresis in a recently introduced gel (polyacrylamide cross-linked with allyl-b-cyclodextrin), (ii) proteins by electrophoretic molecular-sieving in a polymer solution supplemented with SDS, (iii) DNA fragments by electrophoresis in the above gel, (iv) virus particles in this gel, as well as in free buffer and (v) bacteria... (More)
Recently we described the design of a hybrid microdevice for micro(nano)electrophoresis and electrochromatography, discussed its advantages and disadvantages compared to conventional microdevices and presented a few applications with lowmolecular-weight samples. In this paper, we demonstrate the broad application range of this device using UV-based analyses of (i) peptides by free-zone electrophoresis and

electrophoresis in a recently introduced gel (polyacrylamide cross-linked with allyl-b-cyclodextrin), (ii) proteins by electrophoretic molecular-sieving in a polymer solution supplemented with SDS, (iii) DNA fragments by electrophoresis in the above gel, (iv) virus particles in this gel, as well as in free buffer and (v) bacteria in free buffer. To illustrate the advantages of the hybrid microdevice we can mention that electrophoresis of proteins in a polymer-containing buffer, supplemented with sodium dodecyl

sulfate (SDS), in a 4.30 (2.75) cm long channel gave a resolution similar to that in conventional capillary electrophoresis in a 23.5 (18.6) cm long capillary and analysis times which were 15-fold shorter. (Less)
Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Bacteria, DNA, Free-zone electrophoresis, Gel electrophoresis, Microdevice chip, Miniaturization, Peptides, Proteins, Virus
in
Electrophoresis
volume
24
issue
21
pages
3815 - 3820
publisher
John Wiley & Sons
external identifiers
  • scopus:0346503931
ISSN
0173-0835
DOI
10.1002/elps.200305622
language
English
LU publication?
no
id
08d6b448-0f1b-400f-b284-c9dae49577e4 (old id 3516231)
date added to LUP
2013-02-25 10:26:52
date last changed
2017-01-01 07:38:13
@article{08d6b448-0f1b-400f-b284-c9dae49577e4,
  abstract     = {Recently we described the design of a hybrid microdevice for micro(nano)electrophoresis and electrochromatography, discussed its advantages and disadvantages compared to conventional microdevices and presented a few applications with lowmolecular-weight samples. In this paper, we demonstrate the broad application range of this device using UV-based analyses of (i) peptides by free-zone electrophoresis and<br/><br>
electrophoresis in a recently introduced gel (polyacrylamide cross-linked with allyl-b-cyclodextrin), (ii) proteins by electrophoretic molecular-sieving in a polymer solution supplemented with SDS, (iii) DNA fragments by electrophoresis in the above gel, (iv) virus particles in this gel, as well as in free buffer and (v) bacteria in free buffer. To illustrate the advantages of the hybrid microdevice we can mention that electrophoresis of proteins in a polymer-containing buffer, supplemented with sodium dodecyl<br/><br>
sulfate (SDS), in a 4.30 (2.75) cm long channel gave a resolution similar to that in conventional capillary electrophoresis in a 23.5 (18.6) cm long capillary and analysis times which were 15-fold shorter.},
  author       = {Végvári, Ákos and Hjertén, Stellan},
  issn         = {0173-0835},
  keyword      = {Bacteria,DNA,Free-zone electrophoresis,Gel electrophoresis,Microdevice chip,Miniaturization,Peptides,Proteins,Virus},
  language     = {eng},
  number       = {21},
  pages        = {3815--3820},
  publisher    = {John Wiley & Sons},
  series       = {Electrophoresis},
  title        = {Hybrid Microdevice Electrophoretic of Peptides, Proteins, DNA, Viruses and Bacteria in Various Separations Media Using UV-Detection},
  url          = {http://dx.doi.org/10.1002/elps.200305622},
  volume       = {24},
  year         = {2003},
}