Stable Homogeneous Gel for Molecular-Sieving of DNA Fragments in Capillary Electrophoresis
(2002) In Journal of Chromatography A 960(1-2). p.221-227- Abstract
- A polyacrylamide gel crosslinked with allyl-b-cyclodextrin can be used repeatedly for several weeks for the separation of DNA fragments, since bubbles are not generated during a run. Allyl-b-cyclodextrin can easily be synthesized in one step from allylglycidylether and b-cyclodextrin. The plate numbers for DNA fragments, up to about 1500 bp, are high: for the 21 separation of pBR322/HaeIII fragments they were in the range 450 000–1 600 000 m . The resolution was almost independent of the concentration of the crosslinker (allyl-b-cyclodextrin) — in sharp contrast to gels crosslinked with N,N9-methylenebisacrylamide.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3516236
- author
- Végvári, Ákos LU and Hjertén, Stellan
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Capillary electrophoresis, DNA, Polyacrylamide, Allyl-b-cyclodextrin
- in
- Journal of Chromatography A
- volume
- 960
- issue
- 1-2
- pages
- 221 - 227
- publisher
- Elsevier
- external identifiers
-
- scopus:0037172696
- ISSN
- 0021-9673
- DOI
- 10.1016/S0021-9673(01)01436-4,
- language
- English
- LU publication?
- no
- id
- cc510027-4876-49d2-a341-fdf6e796c72d (old id 3516236)
- date added to LUP
- 2016-04-04 09:17:51
- date last changed
- 2022-02-13 08:40:09
@article{cc510027-4876-49d2-a341-fdf6e796c72d, abstract = {{A polyacrylamide gel crosslinked with allyl-b-cyclodextrin can be used repeatedly for several weeks for the separation of DNA fragments, since bubbles are not generated during a run. Allyl-b-cyclodextrin can easily be synthesized in one step from allylglycidylether and b-cyclodextrin. The plate numbers for DNA fragments, up to about 1500 bp, are high: for the 21 separation of pBR322/HaeIII fragments they were in the range 450 000–1 600 000 m . The resolution was almost independent of the concentration of the crosslinker (allyl-b-cyclodextrin) — in sharp contrast to gels crosslinked with N,N9-methylenebisacrylamide.}}, author = {{Végvári, Ákos and Hjertén, Stellan}}, issn = {{0021-9673}}, keywords = {{Capillary electrophoresis; DNA; Polyacrylamide; Allyl-b-cyclodextrin}}, language = {{eng}}, number = {{1-2}}, pages = {{221--227}}, publisher = {{Elsevier}}, series = {{Journal of Chromatography A}}, title = {{Stable Homogeneous Gel for Molecular-Sieving of DNA Fragments in Capillary Electrophoresis}}, url = {{http://dx.doi.org/10.1016/S0021-9673(01)01436-4,}}, doi = {{10.1016/S0021-9673(01)01436-4,}}, volume = {{960}}, year = {{2002}}, }