Chiral Separation of Amino Acids by Ligand-Exchange Electrochromatography Using Continuous Beds
(2000) In Electrophoresis 21(15). p.3141-3144- Abstract
- A chiral ligand-exchange phase for capillary electrochromatography based on continu-ous bed technology was developed. The chiral stationary phase is prepared by a one- step in situ copolymerization procedure using methacrylamide, piperazine diacryla- mide, vinylsulfonic acid and N-(2-hydroxy-3-allyloxypropyl)-L-4-hydroxyproline. These chiral continuous beds are inexpensive and easy to prepare. They also have several advantages over silica-based packed capillaries. Since the bed is covalently attached to the capillary wall, no frit is required. The applicability of this new approach to the chi- ral separation of underivatized amino acids is demonstrated.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3516318
- author
- Schmid, Martin G. ; Grobuschek, Nina ; Tuscher, Claudia ; Gübitz, Gerald ; Végvári, Ákos LU ; Machtejevas, Egidijus ; Maruska, Audrius and Hjertén, Stellan
- publishing date
- 2000
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Capillary electrochromatography, Enantiomer separation, Ligand-exchange, Continuous beds, Chiral beds, Monolithic phases
- in
- Electrophoresis
- volume
- 21
- issue
- 15
- pages
- 3141 - 3144
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- scopus:0033799750
- ISSN
- 0173-0835
- DOI
- 10.1002/1522-2683(20000901)21:15<3141::AID-ELPS3141>3.0.CO;2-T
- language
- English
- LU publication?
- no
- id
- 707df937-78de-4aab-b459-eed16410d3c1 (old id 3516318)
- date added to LUP
- 2016-04-04 08:56:23
- date last changed
- 2022-03-15 17:21:46
@article{707df937-78de-4aab-b459-eed16410d3c1, abstract = {{A chiral ligand-exchange phase for capillary electrochromatography based on continu-ous bed technology was developed. The chiral stationary phase is prepared by a one- step in situ copolymerization procedure using methacrylamide, piperazine diacryla- mide, vinylsulfonic acid and N-(2-hydroxy-3-allyloxypropyl)-L-4-hydroxyproline. These chiral continuous beds are inexpensive and easy to prepare. They also have several advantages over silica-based packed capillaries. Since the bed is covalently attached to the capillary wall, no frit is required. The applicability of this new approach to the chi- ral separation of underivatized amino acids is demonstrated.}}, author = {{Schmid, Martin G. and Grobuschek, Nina and Tuscher, Claudia and Gübitz, Gerald and Végvári, Ákos and Machtejevas, Egidijus and Maruska, Audrius and Hjertén, Stellan}}, issn = {{0173-0835}}, keywords = {{Capillary electrochromatography; Enantiomer separation; Ligand-exchange; Continuous beds; Chiral beds; Monolithic phases}}, language = {{eng}}, number = {{15}}, pages = {{3141--3144}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Electrophoresis}}, title = {{Chiral Separation of Amino Acids by Ligand-Exchange Electrochromatography Using Continuous Beds}}, url = {{http://dx.doi.org/10.1002/1522-2683(20000901)21:15<3141::AID-ELPS3141>3.0.CO;2-T}}, doi = {{10.1002/1522-2683(20000901)21:15<3141::AID-ELPS3141>3.0.CO;2-T}}, volume = {{21}}, year = {{2000}}, }