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Chiral Separation of Amino Acids by Ligand-Exchange Electrochromatography Using Continuous Beds

Schmid, Martin G.; Grobuschek, Nina; Tuscher, Claudia; Gübitz, Gerald; Végvári, Ákos LU ; Machtejevas, Egidijus; Maruska, Audrius and Hjertén, Stellan (2000) In Electrophoresis 21(15). p.3141-3144
Abstract
A chiral ligand-exchange phase for capillary electrochromatography based on continu-ous bed technology was developed. The chiral stationary phase is prepared by a one- step in situ copolymerization procedure using methacrylamide, piperazine diacryla- mide, vinylsulfonic acid and N-(2-hydroxy-3-allyloxypropyl)-L-4-hydroxyproline. These chiral continuous beds are inexpensive and easy to prepare. They also have several advantages over silica-based packed capillaries. Since the bed is covalently attached to the capillary wall, no frit is required. The applicability of this new approach to the chi- ral separation of underivatized amino acids is demonstrated.
Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Capillary electrochromatography, Enantiomer separation, Ligand-exchange, Continuous beds, Chiral beds, Monolithic phases
in
Electrophoresis
volume
21
issue
15
pages
3141 - 3144
publisher
John Wiley & Sons
external identifiers
  • scopus:0033799750
ISSN
0173-0835
DOI
10.1002/1522-2683(20000901)21:15<3141::AID-ELPS3141>3.0.CO;2-T
language
English
LU publication?
no
id
707df937-78de-4aab-b459-eed16410d3c1 (old id 3516318)
date added to LUP
2013-02-25 11:02:50
date last changed
2017-06-11 04:43:31
@article{707df937-78de-4aab-b459-eed16410d3c1,
  abstract     = {A chiral ligand-exchange phase for capillary electrochromatography based on continu-ous bed technology was developed. The chiral stationary phase is prepared by a one- step in situ copolymerization procedure using methacrylamide, piperazine diacryla- mide, vinylsulfonic acid and N-(2-hydroxy-3-allyloxypropyl)-L-4-hydroxyproline. These chiral continuous beds are inexpensive and easy to prepare. They also have several advantages over silica-based packed capillaries. Since the bed is covalently attached to the capillary wall, no frit is required. The applicability of this new approach to the chi- ral separation of underivatized amino acids is demonstrated.},
  author       = {Schmid, Martin G. and Grobuschek, Nina and Tuscher, Claudia and Gübitz, Gerald and Végvári, Ákos and Machtejevas, Egidijus and Maruska, Audrius and Hjertén, Stellan},
  issn         = {0173-0835},
  keyword      = {Capillary electrochromatography,Enantiomer separation,Ligand-exchange,Continuous beds,Chiral beds,Monolithic phases},
  language     = {eng},
  number       = {15},
  pages        = {3141--3144},
  publisher    = {John Wiley & Sons},
  series       = {Electrophoresis},
  title        = {Chiral Separation of Amino Acids by Ligand-Exchange Electrochromatography Using Continuous Beds},
  url          = {http://dx.doi.org/10.1002/1522-2683(20000901)21:15<3141::AID-ELPS3141>3.0.CO;2-T},
  volume       = {21},
  year         = {2000},
}