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Lack of endogenous annexin A1 exacerbates NLRP3 inflammasome response in macrophages

Sanches, José Marcos LU ; Branco, Laura ; Oliani, Sonia ; Bortoluci, Karina ; Moreira, Vanessa and Gil, Cristiane (2019) International Conference on Annexin Biology p.67-67
Abstract
This study evalluated the role of endogenous Annexin A1 in the regulation of the
NLRP3 inflammasome. B57bl/6 wild-type (WT) and knockouts for ANXA1 (ANXA1-
/-) received intraperitoneal injection of 1.5% starch solution for macrophages
recruitment. NLRP3 was activated by priming cells with lipopolysaccharide (500
ng/mL) for 3 hours, followed by nigericin (10 µM for 1 hour) or ATP (5 mM for 30
minutes). After the treatments, the cells and supernatant were collected for ELISA,
western blotting, cell viability by MTT, ultrastructural immunocytochemistry
and, lipidomic analysis. As expected, nigericin and ATP administration decreased
macrophage viability, more pronounced in the ANXA1-/- cells, compared... (More)
This study evalluated the role of endogenous Annexin A1 in the regulation of the
NLRP3 inflammasome. B57bl/6 wild-type (WT) and knockouts for ANXA1 (ANXA1-
/-) received intraperitoneal injection of 1.5% starch solution for macrophages
recruitment. NLRP3 was activated by priming cells with lipopolysaccharide (500
ng/mL) for 3 hours, followed by nigericin (10 µM for 1 hour) or ATP (5 mM for 30
minutes). After the treatments, the cells and supernatant were collected for ELISA,
western blotting, cell viability by MTT, ultrastructural immunocytochemistry
and, lipidomic analysis. As expected, nigericin and ATP administration decreased
macrophage viability, more pronounced in the ANXA1-/- cells, compared with
control and only LPS-treated cells (p < 0.001). Additionally, treatment with nigericin
and ATP produced a marked release of IL-1β in ANXA1-/- macrophages compared
to the other groups. Ultrastructural analysis demonstrated a high expression of
NLRP3 in the nigericin-activated ANXA1-/- macrophages. WT cells showed points of
co-localization of ANXA1 and NLRP3. Lipidomic analysis of macrophages evidenced
a completed different lipid profile between WT and ANXA1-/- supernatant cells.
In WT, nigericin administration induced a pronounced release of eicosanoids and
prostaglandins, while ANXA1-/- showed precursors of prostaglandin and some
ceramides. Altogether, our results suggested that endogenous ANXA1 regulates the
NLRP3-derived IL-1β in macrophages. (Less)
Please use this url to cite or link to this publication:
author
; ; ; ; and
publishing date
type
Contribution to conference
publication status
published
pages
67 - 67
conference name
International Conference on Annexin Biology
conference location
Mûnster, Germany
conference dates
2019-09-15 - 2019-09-18
language
English
LU publication?
no
id
35168176-e2f2-4ab9-9269-0c200fa5b7df
date added to LUP
2021-03-31 14:37:45
date last changed
2021-12-15 14:12:38
@misc{35168176-e2f2-4ab9-9269-0c200fa5b7df,
  abstract     = {{This study evalluated the role of endogenous Annexin A1 in the regulation of the<br/>NLRP3 inflammasome. B57bl/6 wild-type (WT) and knockouts for ANXA1 (ANXA1-<br/>/-) received intraperitoneal injection of 1.5% starch solution for macrophages<br/>recruitment. NLRP3 was activated by priming cells with lipopolysaccharide (500<br/>ng/mL) for 3 hours, followed by nigericin (10 µM for 1 hour) or ATP (5 mM for 30<br/>minutes). After the treatments, the cells and supernatant were collected for ELISA,<br/>western blotting, cell viability by MTT, ultrastructural immunocytochemistry<br/>and, lipidomic analysis. As expected, nigericin and ATP administration decreased<br/>macrophage viability, more pronounced in the ANXA1-/- cells, compared with<br/>control and only LPS-treated cells (p &lt; 0.001). Additionally, treatment with nigericin<br/>and ATP produced a marked release of IL-1β in ANXA1-/- macrophages compared<br/>to the other groups. Ultrastructural analysis demonstrated a high expression of<br/>NLRP3 in the nigericin-activated ANXA1-/- macrophages. WT cells showed points of<br/>co-localization of ANXA1 and NLRP3. Lipidomic analysis of macrophages evidenced<br/>a completed different lipid profile between WT and ANXA1-/- supernatant cells.<br/>In WT, nigericin administration induced a pronounced release of eicosanoids and<br/>prostaglandins, while ANXA1-/- showed precursors of prostaglandin and some<br/>ceramides. Altogether, our results suggested that endogenous ANXA1 regulates the<br/>NLRP3-derived IL-1β in macrophages.}},
  author       = {{Sanches, José Marcos and Branco, Laura and Oliani, Sonia and Bortoluci, Karina and Moreira, Vanessa and Gil, Cristiane}},
  language     = {{eng}},
  pages        = {{67--67}},
  title        = {{Lack of endogenous annexin A1 exacerbates NLRP3 inflammasome response in macrophages}},
  url          = {{https://lup.lub.lu.se/search/files/110999340/Abstract_Book_Annexin_2019_corrected_abst_4_copy.pdf}},
  year         = {{2019}},
}