Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Purification and cloning of type A/B hnRNP protein involved in transcriptional activation from the rat spi 2 gene GAGA box

Leverrier, S. ; Cinato, E. ; Paul, C. ; Derancourt, J. ; Bemark, M. LU orcid ; Leanderson, T. LU and Legraverend, C. (2000) In Biological Chemistry 381(11). p.1031-1040
Abstract

The GAGA box of the rat serine protease inhibitor 2 (spi 2) genes not only acts as a basal promoter element, but also mediates transcriptional activation by growth hormone and interleukin-6. The GAGA box is separated from the TATA box by only 12 bp, and this close association is required for efficient transcription. Hence, the GAGA box may influence transcription efficiency through interactions between GAGA box binding proteins and some components of the RNA polymerase II complex. Here we report the cloning of two GAGA box-binding proteins termed p38 and p40, that belong to the type A/B heterogeneous nuclear ribonucleoprotein subgroup. GAGA box mutations that diminish the affinity for p38 and p40 decrease basal and GH-induced reporter... (More)

The GAGA box of the rat serine protease inhibitor 2 (spi 2) genes not only acts as a basal promoter element, but also mediates transcriptional activation by growth hormone and interleukin-6. The GAGA box is separated from the TATA box by only 12 bp, and this close association is required for efficient transcription. Hence, the GAGA box may influence transcription efficiency through interactions between GAGA box binding proteins and some components of the RNA polymerase II complex. Here we report the cloning of two GAGA box-binding proteins termed p38 and p40, that belong to the type A/B heterogeneous nuclear ribonucleoprotein subgroup. GAGA box mutations that diminish the affinity for p38 and p40 decrease basal and GH-induced reporter gene expression. Furthermore, nuclear extracts depleted of p38 and p40 can no longer support GAGA box-dependent in vitro transcription. Therefore, two polypeptides previously assigned to a family of RNA processing proteins also act as DNA-binding, promoter-specific transcription factors.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
GAGA box, Serine protease inhibitor, Trancription factor, Type A/B hnRNP
in
Biological Chemistry
volume
381
issue
11
pages
1031 - 1040
publisher
De Gruyter
external identifiers
  • pmid:11154060
  • scopus:0034533006
ISSN
1431-6730
DOI
10.1515/BC.2000.127
language
English
LU publication?
yes
additional info
Funding Information: Sabrina Leverrier was supported by a grant from the Ministère de l’Education Supérieure et de la Recherche. M. Bemark and T. Leanderson were supported by grants from the Swedish Society and the Swedish Medical Research Council. We thank Vincent Christoffels for the gift of CPS-I promoter deletion constructs and Dominique Joubert for her critical comments on the manuscript.
id
35204378-4640-45f9-a2da-3c94e263ed62
date added to LUP
2023-12-06 17:14:33
date last changed
2024-10-05 05:03:10
@article{35204378-4640-45f9-a2da-3c94e263ed62,
  abstract     = {{<p>The GAGA box of the rat serine protease inhibitor 2 (spi 2) genes not only acts as a basal promoter element, but also mediates transcriptional activation by growth hormone and interleukin-6. The GAGA box is separated from the TATA box by only 12 bp, and this close association is required for efficient transcription. Hence, the GAGA box may influence transcription efficiency through interactions between GAGA box binding proteins and some components of the RNA polymerase II complex. Here we report the cloning of two GAGA box-binding proteins termed p38 and p40, that belong to the type A/B heterogeneous nuclear ribonucleoprotein subgroup. GAGA box mutations that diminish the affinity for p38 and p40 decrease basal and GH-induced reporter gene expression. Furthermore, nuclear extracts depleted of p38 and p40 can no longer support GAGA box-dependent in vitro transcription. Therefore, two polypeptides previously assigned to a family of RNA processing proteins also act as DNA-binding, promoter-specific transcription factors.</p>}},
  author       = {{Leverrier, S. and Cinato, E. and Paul, C. and Derancourt, J. and Bemark, M. and Leanderson, T. and Legraverend, C.}},
  issn         = {{1431-6730}},
  keywords     = {{GAGA box; Serine protease inhibitor; Trancription factor; Type A/B hnRNP}},
  language     = {{eng}},
  number       = {{11}},
  pages        = {{1031--1040}},
  publisher    = {{De Gruyter}},
  series       = {{Biological Chemistry}},
  title        = {{Purification and cloning of type A/B hnRNP protein involved in transcriptional activation from the rat spi 2 gene GAGA box}},
  url          = {{http://dx.doi.org/10.1515/BC.2000.127}},
  doi          = {{10.1515/BC.2000.127}},
  volume       = {{381}},
  year         = {{2000}},
}