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Designs of bioreactor systems for solvent-free lipase-catalyzed synthesis of fructose–oleic acid esters

Pyo, Sang-Hyun LU and Hayes, Douglas G. (2009) In JAOCS, Journal of the American Oil Chemists' Society 86(6). p.521-529
Abstract
Fructose–oleic acid esters, biodegradable, biocompatible and biobased surfactants and value-added products were synthesized under solvent-free conditions at 65 °C in stirred-batch mode and using several different bioreactor systems. For a stirred-tank bioreactor (STBR) using fed-batch fructose addition and 5.0 wt.% immobilized Rhizomucor miehei lipase (Lipozyme® IM, Novozymes, Franklinton, NC), the conversion yield was over 80%, and the initial rate of the reaction was comparable to previously obtained results using tert-butanol during the initial phase. The bioreactor systems contained a packed “desorption” column (DC) containing fructose crystals and silica gel for delivery of saccharide, and either a STBR or packed-bed bioreactor... (More)
Fructose–oleic acid esters, biodegradable, biocompatible and biobased surfactants and value-added products were synthesized under solvent-free conditions at 65 °C in stirred-batch mode and using several different bioreactor systems. For a stirred-tank bioreactor (STBR) using fed-batch fructose addition and 5.0 wt.% immobilized Rhizomucor miehei lipase (Lipozyme® IM, Novozymes, Franklinton, NC), the conversion yield was over 80%, and the initial rate of the reaction was comparable to previously obtained results using tert-butanol during the initial phase. The bioreactor systems contained a packed “desorption” column (DC) containing fructose crystals and silica gel for delivery of saccharide, and either a STBR or packed-bed bioreactor (PBBR). The liquid stream, initially containing oleic acid and a mixture of fructose–oleic acid esters at a ratio of 75/25 w/w, was continuously recirculated throughout the system … (Less)
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author
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publishing date
type
Contribution to journal
publication status
published
in
JAOCS, Journal of the American Oil Chemists' Society
volume
86
issue
6
pages
521 - 529
publisher
John Wiley & Sons Inc.
external identifiers
  • scopus:67349123828
ISSN
0003-021X
DOI
10.1007/s11746-009-1381-8
language
English
LU publication?
no
id
35778bd2-f127-4893-ae18-1c2406576deb
date added to LUP
2025-09-05 18:17:50
date last changed
2025-09-22 13:11:20
@article{35778bd2-f127-4893-ae18-1c2406576deb,
  abstract     = {{Fructose–oleic acid esters, biodegradable, biocompatible and biobased surfactants and value-added products were synthesized under solvent-free conditions at 65 °C in stirred-batch mode and using several different bioreactor systems. For a stirred-tank bioreactor (STBR) using fed-batch fructose addition and 5.0 wt.% immobilized Rhizomucor miehei lipase (Lipozyme® IM, Novozymes, Franklinton, NC), the conversion yield was over 80%, and the initial rate of the reaction was comparable to previously obtained results using tert-butanol during the initial phase. The bioreactor systems contained a packed “desorption” column (DC) containing fructose crystals and silica gel for delivery of saccharide, and either a STBR or packed-bed bioreactor (PBBR). The liquid stream, initially containing oleic acid and a mixture of fructose–oleic acid esters at a ratio of 75/25 w/w, was continuously recirculated throughout the system …}},
  author       = {{Pyo, Sang-Hyun and Hayes, Douglas G.}},
  issn         = {{0003-021X}},
  language     = {{eng}},
  number       = {{6}},
  pages        = {{521--529}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{JAOCS, Journal of the American Oil Chemists' Society}},
  title        = {{Designs of bioreactor systems for solvent-free lipase-catalyzed synthesis of fructose–oleic acid esters}},
  url          = {{http://dx.doi.org/10.1007/s11746-009-1381-8}},
  doi          = {{10.1007/s11746-009-1381-8}},
  volume       = {{86}},
  year         = {{2009}},
}