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Cloning and functional expression of the guinea pig neuropeptide Y Y2 receptor

Sharma, P ; Holmberg, S K ; Eriksson, H ; Beck-Sickinger, A G ; Grundemar, L LU and Larhammar, D (1998) In Regulatory Peptides 75-76. p.23-28
Abstract

Five neuropeptide Y (NPY) receptor subtypes have been cloned in mammals. The degree of sequence conservation differs considerably between subtypes as well as between evolutionary lineages. To shed further light on this, we have cloned the five NPY receptors in the guinea pig. Here, we report the cloning of the guinea pig Y2 receptor. The Y2 receptor is generally highly conserved, with 90-95% identity between different orders of mammals, including the guinea pig. The guinea pig receptor has a divergent cytoplasmic tail, indicating possible differences in regulation of signalling and/or down regulation. COS-7 cells transiently transfected with the gpY2 receptor show saturable 125I-PYY binding with a Kd = 6 pM. In displacement experiments,... (More)

Five neuropeptide Y (NPY) receptor subtypes have been cloned in mammals. The degree of sequence conservation differs considerably between subtypes as well as between evolutionary lineages. To shed further light on this, we have cloned the five NPY receptors in the guinea pig. Here, we report the cloning of the guinea pig Y2 receptor. The Y2 receptor is generally highly conserved, with 90-95% identity between different orders of mammals, including the guinea pig. The guinea pig receptor has a divergent cytoplasmic tail, indicating possible differences in regulation of signalling and/or down regulation. COS-7 cells transiently transfected with the gpY2 receptor show saturable 125I-PYY binding with a Kd = 6 pM. In displacement experiments, the gpY2 receptor was similar to the human and rat receptors with the following rank order of potencies: pNPY > pPYY > pNPY13-36 = pNPY22-36 > [Leu31Pro34]NPY > BIBP3226. Thus, the guinea pig Y2 receptor is well conserved in comparison with human and rat with regard to both amino acid sequence and pharmacological profile.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Amino Acid Sequence, Animals, Arginine/analogs & derivatives, COS Cells, Cloning, Molecular, Conserved Sequence, Gene Expression, Guinea Pigs, Humans, In Vitro Techniques, Kinetics, Molecular Sequence Data, Neuropeptide Y/analogs & derivatives, Peptide Fragments/metabolism, Peptide YY/metabolism, Rats, Receptors, Neuropeptide Y/chemistry, Recombinant Proteins/chemistry, Sequence Homology, Amino Acid, Transfection
in
Regulatory Peptides
volume
75-76
pages
23 - 28
publisher
Elsevier
external identifiers
  • scopus:0032566822
  • pmid:9802390
ISSN
0167-0115
DOI
10.1016/s0167-0115(98)00049-4
language
English
LU publication?
yes
id
359471b0-6afa-428b-b61e-b387d146b9e5
date added to LUP
2019-09-03 13:55:11
date last changed
2025-04-04 15:21:56
@article{359471b0-6afa-428b-b61e-b387d146b9e5,
  abstract     = {{<p>Five neuropeptide Y (NPY) receptor subtypes have been cloned in mammals. The degree of sequence conservation differs considerably between subtypes as well as between evolutionary lineages. To shed further light on this, we have cloned the five NPY receptors in the guinea pig. Here, we report the cloning of the guinea pig Y2 receptor. The Y2 receptor is generally highly conserved, with 90-95% identity between different orders of mammals, including the guinea pig. The guinea pig receptor has a divergent cytoplasmic tail, indicating possible differences in regulation of signalling and/or down regulation. COS-7 cells transiently transfected with the gpY2 receptor show saturable 125I-PYY binding with a Kd = 6 pM. In displacement experiments, the gpY2 receptor was similar to the human and rat receptors with the following rank order of potencies: pNPY &gt; pPYY &gt; pNPY13-36 = pNPY22-36 &gt; [Leu31Pro34]NPY &gt; BIBP3226. Thus, the guinea pig Y2 receptor is well conserved in comparison with human and rat with regard to both amino acid sequence and pharmacological profile.</p>}},
  author       = {{Sharma, P and Holmberg, S K and Eriksson, H and Beck-Sickinger, A G and Grundemar, L and Larhammar, D}},
  issn         = {{0167-0115}},
  keywords     = {{Amino Acid Sequence; Animals; Arginine/analogs & derivatives; COS Cells; Cloning, Molecular; Conserved Sequence; Gene Expression; Guinea Pigs; Humans; In Vitro Techniques; Kinetics; Molecular Sequence Data; Neuropeptide Y/analogs & derivatives; Peptide Fragments/metabolism; Peptide YY/metabolism; Rats; Receptors, Neuropeptide Y/chemistry; Recombinant Proteins/chemistry; Sequence Homology, Amino Acid; Transfection}},
  language     = {{eng}},
  month        = {{09}},
  pages        = {{23--28}},
  publisher    = {{Elsevier}},
  series       = {{Regulatory Peptides}},
  title        = {{Cloning and functional expression of the guinea pig neuropeptide Y Y2 receptor}},
  url          = {{http://dx.doi.org/10.1016/s0167-0115(98)00049-4}},
  doi          = {{10.1016/s0167-0115(98)00049-4}},
  volume       = {{75-76}},
  year         = {{1998}},
}