Investigating the kinetics of the enzymatic depolymerization of polygalacturonic acid in continuous UF-Membrane reactors
(2007) In Biotechnology Progress 23(5). p.1111-1117- Abstract
- A stirred tank membrane reactor is used to study the kinetics of polygalacturonic acid (PGA) enzymatic hydrolysis. The reactor operates in semicontinuous configuration: the native biopolymer is loaded at the initial time and the system is continuously fed with the buffer. The effect of retention time (from 10 1 to 142 min) and membrane molecular weight cutoff (from I to 30 kDa) on the rate of permeable oligomers production is investigated. Reaction products are clustered in two different classes, those sized below the membrane cutoff and those above. The reducing power measured in the permeate is used as an estimate of total product concentration. The characteristic breakdown times range from 40 to 100 min. The overall kinetics obeys a... (More)
- A stirred tank membrane reactor is used to study the kinetics of polygalacturonic acid (PGA) enzymatic hydrolysis. The reactor operates in semicontinuous configuration: the native biopolymer is loaded at the initial time and the system is continuously fed with the buffer. The effect of retention time (from 10 1 to 142 min) and membrane molecular weight cutoff (from I to 30 kDa) on the rate of permeable oligomers production is investigated. Reaction products are clustered in two different classes, those sized below the membrane cutoff and those above. The reducing power measured in the permeate is used as an estimate of total product concentration. The characteristic breakdown times range from 40 to 100 min. The overall kinetics obeys a first-order law with a characteristic time estimated to 24 min. New mathematical data handling are developed and illustrated using the experimental data obtained. Finally, the body of the experimental results suggests useful indications (reactor productivity, breakdown induction period) for implementing the bioprocess at the industrial scale. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3917196
- author
- Gallifuoco, Alberto ; Cantarella, Maria and Marucci, Mariagrazia LU
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biotechnology Progress
- volume
- 23
- issue
- 5
- pages
- 1111 - 1117
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000250010000018
- scopus:35348926825
- ISSN
- 1520-6033
- DOI
- 10.1021/bp070177y
- language
- English
- LU publication?
- no
- id
- 36054928-0680-4841-a56a-f150dbe4113b (old id 3917196)
- date added to LUP
- 2016-04-01 16:01:47
- date last changed
- 2022-03-22 07:54:21
@article{36054928-0680-4841-a56a-f150dbe4113b,
abstract = {{A stirred tank membrane reactor is used to study the kinetics of polygalacturonic acid (PGA) enzymatic hydrolysis. The reactor operates in semicontinuous configuration: the native biopolymer is loaded at the initial time and the system is continuously fed with the buffer. The effect of retention time (from 10 1 to 142 min) and membrane molecular weight cutoff (from I to 30 kDa) on the rate of permeable oligomers production is investigated. Reaction products are clustered in two different classes, those sized below the membrane cutoff and those above. The reducing power measured in the permeate is used as an estimate of total product concentration. The characteristic breakdown times range from 40 to 100 min. The overall kinetics obeys a first-order law with a characteristic time estimated to 24 min. New mathematical data handling are developed and illustrated using the experimental data obtained. Finally, the body of the experimental results suggests useful indications (reactor productivity, breakdown induction period) for implementing the bioprocess at the industrial scale.}},
author = {{Gallifuoco, Alberto and Cantarella, Maria and Marucci, Mariagrazia}},
issn = {{1520-6033}},
language = {{eng}},
number = {{5}},
pages = {{1111--1117}},
publisher = {{The American Chemical Society (ACS)}},
series = {{Biotechnology Progress}},
title = {{Investigating the kinetics of the enzymatic depolymerization of polygalacturonic acid in continuous UF-Membrane reactors}},
url = {{http://dx.doi.org/10.1021/bp070177y}},
doi = {{10.1021/bp070177y}},
volume = {{23}},
year = {{2007}},
}