Developing defined substrates for stem cell culture and differentiation
Hagbard, Louise ; Cameron, Katherine ; August, Paul ; Penton, Christopher ; Parmar, Malin LU
; Hay, David C.
and Kallur, Therése
(2018)
In Philosophical Transactions of the Royal Society B: Biological Sciences
373(1750).
- Abstract
Over the past few decades, a variety of different reagents for stem cell maintenance and differentiation have been commercialized. These reagents share a common goal in facilitating the manufacture of products suitable for cell therapy while reducing the amount of non-defined components. Lessons from developmental biology have identified signalling molecules that can guide the differentiation process in vitro, but less attention has been paid to the extracellular matrix used. With the introduction of more biologically relevant and defined matrices, that better mimic specific cell niches, researchers now have powerful resources to fine-tune their in vitro differentiation systems, which may allow the manufacture of therapeutically... (More)
Over the past few decades, a variety of different reagents for stem cell maintenance and differentiation have been commercialized. These reagents share a common goal in facilitating the manufacture of products suitable for cell therapy while reducing the amount of non-defined components. Lessons from developmental biology have identified signalling molecules that can guide the differentiation process in vitro, but less attention has been paid to the extracellular matrix used. With the introduction of more biologically relevant and defined matrices, that better mimic specific cell niches, researchers now have powerful resources to fine-tune their in vitro differentiation systems, which may allow the manufacture of therapeutically relevant cell types. In this review article, we revisit the basics of the extracellular matrix, and explore the important role of the cell –matrix interaction. We focus on laminin proteins because they help to maintain pluripotency and drive cell fate specification. This article is part of the theme issue ‘Designer human tissue: coming to a lab near you’.
(Less)
- author
- Hagbard, Louise
; Cameron, Katherine
; August, Paul
; Penton, Christopher
; Parmar, Malin
LU
; Hay, David C.
and Kallur, Therése
- organization
- publishing date
- 2018-07-05
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Cell culture, Cell therapy, Defined substrates, Human pluripotent stem cells, Human recombinant laminins
- in
- Philosophical Transactions of the Royal Society B: Biological Sciences
- volume
- 373
- issue
- 1750
- article number
- 20170230
- publisher
- Royal Society Publishing
- external identifiers
-
- scopus:85047351319
- pmid:29786564
- ISSN
- 1471-2970
- DOI
- 10.1098/rstb.2017.0230
- language
- English
- LU publication?
- yes
- id
- 3614cfa8-2f6a-41c8-8901-2983f396040d
- date added to LUP
- 2018-05-31 14:45:49
- date last changed
- 2024-05-27 12:29:25
@article{3614cfa8-2f6a-41c8-8901-2983f396040d,
abstract = {{<p>Over the past few decades, a variety of different reagents for stem cell maintenance and differentiation have been commercialized. These reagents share a common goal in facilitating the manufacture of products suitable for cell therapy while reducing the amount of non-defined components. Lessons from developmental biology have identified signalling molecules that can guide the differentiation process in vitro, but less attention has been paid to the extracellular matrix used. With the introduction of more biologically relevant and defined matrices, that better mimic specific cell niches, researchers now have powerful resources to fine-tune their in vitro differentiation systems, which may allow the manufacture of therapeutically relevant cell types. In this review article, we revisit the basics of the extracellular matrix, and explore the important role of the cell –matrix interaction. We focus on laminin proteins because they help to maintain pluripotency and drive cell fate specification. This article is part of the theme issue ‘Designer human tissue: coming to a lab near you’.</p>}},
author = {{Hagbard, Louise and Cameron, Katherine and August, Paul and Penton, Christopher and Parmar, Malin and Hay, David C. and Kallur, Therése}},
issn = {{1471-2970}},
keywords = {{Cell culture; Cell therapy; Defined substrates; Human pluripotent stem cells; Human recombinant laminins}},
language = {{eng}},
month = {{07}},
number = {{1750}},
publisher = {{Royal Society Publishing}},
series = {{Philosophical Transactions of the Royal Society B: Biological Sciences}},
title = {{Developing defined substrates for stem cell culture and differentiation}},
url = {{http://dx.doi.org/10.1098/rstb.2017.0230}},
doi = {{10.1098/rstb.2017.0230}},
volume = {{373}},
year = {{2018}},
}