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Developing defined substrates for stem cell culture and differentiation

Hagbard, Louise ; Cameron, Katherine ; August, Paul ; Penton, Christopher ; Parmar, Malin LU orcid ; Hay, David C. and Kallur, Therése (2018) In Philosophical Transactions of the Royal Society B: Biological Sciences 373(1750).
Abstract

Over the past few decades, a variety of different reagents for stem cell maintenance and differentiation have been commercialized. These reagents share a common goal in facilitating the manufacture of products suitable for cell therapy while reducing the amount of non-defined components. Lessons from developmental biology have identified signalling molecules that can guide the differentiation process in vitro, but less attention has been paid to the extracellular matrix used. With the introduction of more biologically relevant and defined matrices, that better mimic specific cell niches, researchers now have powerful resources to fine-tune their in vitro differentiation systems, which may allow the manufacture of therapeutically... (More)

Over the past few decades, a variety of different reagents for stem cell maintenance and differentiation have been commercialized. These reagents share a common goal in facilitating the manufacture of products suitable for cell therapy while reducing the amount of non-defined components. Lessons from developmental biology have identified signalling molecules that can guide the differentiation process in vitro, but less attention has been paid to the extracellular matrix used. With the introduction of more biologically relevant and defined matrices, that better mimic specific cell niches, researchers now have powerful resources to fine-tune their in vitro differentiation systems, which may allow the manufacture of therapeutically relevant cell types. In this review article, we revisit the basics of the extracellular matrix, and explore the important role of the cell –matrix interaction. We focus on laminin proteins because they help to maintain pluripotency and drive cell fate specification. This article is part of the theme issue ‘Designer human tissue: coming to a lab near you’.

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Please use this url to cite or link to this publication:
author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cell culture, Cell therapy, Defined substrates, Human pluripotent stem cells, Human recombinant laminins
in
Philosophical Transactions of the Royal Society B: Biological Sciences
volume
373
issue
1750
article number
20170230
publisher
Royal Society Publishing
external identifiers
  • scopus:85047351319
  • pmid:29786564
ISSN
1471-2970
DOI
10.1098/rstb.2017.0230
language
English
LU publication?
yes
id
3614cfa8-2f6a-41c8-8901-2983f396040d
date added to LUP
2018-05-31 14:45:49
date last changed
2021-10-10 04:27:46
@article{3614cfa8-2f6a-41c8-8901-2983f396040d,
  abstract     = {<p>Over the past few decades, a variety of different reagents for stem cell maintenance and differentiation have been commercialized. These reagents share a common goal in facilitating the manufacture of products suitable for cell therapy while reducing the amount of non-defined components. Lessons from developmental biology have identified signalling molecules that can guide the differentiation process in vitro, but less attention has been paid to the extracellular matrix used. With the introduction of more biologically relevant and defined matrices, that better mimic specific cell niches, researchers now have powerful resources to fine-tune their in vitro differentiation systems, which may allow the manufacture of therapeutically relevant cell types. In this review article, we revisit the basics of the extracellular matrix, and explore the important role of the cell –matrix interaction. We focus on laminin proteins because they help to maintain pluripotency and drive cell fate specification. This article is part of the theme issue ‘Designer human tissue: coming to a lab near you’.</p>},
  author       = {Hagbard, Louise and Cameron, Katherine and August, Paul and Penton, Christopher and Parmar, Malin and Hay, David C. and Kallur, Therése},
  issn         = {1471-2970},
  language     = {eng},
  month        = {07},
  number       = {1750},
  publisher    = {Royal Society Publishing},
  series       = {Philosophical Transactions of the Royal Society B: Biological Sciences},
  title        = {Developing defined substrates for stem cell culture and differentiation},
  url          = {http://dx.doi.org/10.1098/rstb.2017.0230},
  doi          = {10.1098/rstb.2017.0230},
  volume       = {373},
  year         = {2018},
}