Cadmium, mercury, and lead in kidney cortex are not associated with urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) in living kidney donors.
(2014) In International Archives of Occupational and Environmental Health 87(3). p.315-322- Abstract
- PURPOSE: Cadmium in urine is positively associated with urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) concentrations, a sensitive marker of oxidative DNA damage. We determined whether kidney concentrations of cadmium, mercury, and lead, which may generate oxidative DNA damage, were associated with urinary 8-oxodG or not. METHODS: 8-OxodG was measured in separate 24 h and overnight urine samples from Swedish healthy adult kidney donors (N = 152) using LC-MS/MS. Concentrations of metals were measured in kidney biopsies (N = 109) by ICP-MS. RESULTS: The median 8-oxodG concentrations (adjusted to specific gravity) in 24 h and overnight samples were 13.5 and 15.3 nmol/L; 8-oxodG excretion rates in 24 h and overnight samples were 0.93... (More)
- PURPOSE: Cadmium in urine is positively associated with urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) concentrations, a sensitive marker of oxidative DNA damage. We determined whether kidney concentrations of cadmium, mercury, and lead, which may generate oxidative DNA damage, were associated with urinary 8-oxodG or not. METHODS: 8-OxodG was measured in separate 24 h and overnight urine samples from Swedish healthy adult kidney donors (N = 152) using LC-MS/MS. Concentrations of metals were measured in kidney biopsies (N = 109) by ICP-MS. RESULTS: The median 8-oxodG concentrations (adjusted to specific gravity) in 24 h and overnight samples were 13.5 and 15.3 nmol/L; 8-oxodG excretion rates in 24 h and overnight samples were 0.93 and 0.86 nmol/h. In multivariable linear regression analyses, we did not find any association between 8-oxodG concentrations or rates and elements in the kidney. The 24-h 8-oxodG concentrations were positively associated with serum ferritin (β = 0.048, p < 0.0001), body weight (β = 0.13, p = 0.0019), and inversely with gender (β = -3.34, p = 0.0024). Similar associations with 8-oxodG excretion rates were stronger. Smoking was positively associated with 24-h 8-oxodG excretion rates (β = 0.26, p = 0.0090), but not with overnight samples. CONCLUSIONS: Neither cadmium, nor mercury or lead in the kidney contributed to urinary 8-oxodG concentrations in non-occupationally exposed subjects. The iron status was positively associated with urinary 8-oxodG, particularly in women. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3627595
- author
- Hossain, Mohammad Bakhtiar
; Barregard, Lars
; Sallsten, Gerd
and Broberg Palmgren, Karin
LU
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- International Archives of Occupational and Environmental Health
- volume
- 87
- issue
- 3
- pages
- 315 - 322
- publisher
- Springer
- external identifiers
-
- pmid:23536210
- wos:000332953300010
- scopus:84899407648
- pmid:23536210
- ISSN
- 1432-1246
- DOI
- 10.1007/s00420-013-0863-z
- language
- English
- LU publication?
- yes
- id
- 78cdc1cf-8bf3-4cc1-b587-4f8ced014667 (old id 3627595)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/23536210?dopt=Abstract
- date added to LUP
- 2016-04-01 10:52:31
- date last changed
- 2022-04-04 22:08:24
@article{78cdc1cf-8bf3-4cc1-b587-4f8ced014667, abstract = {{PURPOSE: Cadmium in urine is positively associated with urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) concentrations, a sensitive marker of oxidative DNA damage. We determined whether kidney concentrations of cadmium, mercury, and lead, which may generate oxidative DNA damage, were associated with urinary 8-oxodG or not. METHODS: 8-OxodG was measured in separate 24 h and overnight urine samples from Swedish healthy adult kidney donors (N = 152) using LC-MS/MS. Concentrations of metals were measured in kidney biopsies (N = 109) by ICP-MS. RESULTS: The median 8-oxodG concentrations (adjusted to specific gravity) in 24 h and overnight samples were 13.5 and 15.3 nmol/L; 8-oxodG excretion rates in 24 h and overnight samples were 0.93 and 0.86 nmol/h. In multivariable linear regression analyses, we did not find any association between 8-oxodG concentrations or rates and elements in the kidney. The 24-h 8-oxodG concentrations were positively associated with serum ferritin (β = 0.048, p < 0.0001), body weight (β = 0.13, p = 0.0019), and inversely with gender (β = -3.34, p = 0.0024). Similar associations with 8-oxodG excretion rates were stronger. Smoking was positively associated with 24-h 8-oxodG excretion rates (β = 0.26, p = 0.0090), but not with overnight samples. CONCLUSIONS: Neither cadmium, nor mercury or lead in the kidney contributed to urinary 8-oxodG concentrations in non-occupationally exposed subjects. The iron status was positively associated with urinary 8-oxodG, particularly in women.}}, author = {{Hossain, Mohammad Bakhtiar and Barregard, Lars and Sallsten, Gerd and Broberg Palmgren, Karin}}, issn = {{1432-1246}}, language = {{eng}}, number = {{3}}, pages = {{315--322}}, publisher = {{Springer}}, series = {{International Archives of Occupational and Environmental Health}}, title = {{Cadmium, mercury, and lead in kidney cortex are not associated with urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) in living kidney donors.}}, url = {{http://dx.doi.org/10.1007/s00420-013-0863-z}}, doi = {{10.1007/s00420-013-0863-z}}, volume = {{87}}, year = {{2014}}, }