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Multimodal Interaction Chromatography for the Investigation and Separation of Proteins

Kallberg, Kristian LU (2013)
Abstract
Minor structural changes on the surface of proteins can have tremendous impact on their biological properties. When producing therapeutic proteins it is therefore important to verify that the generated protein is the desired one. Small variations such as glycosylations or amino acid substitutions can mean the difference between a functional, defective or even toxic product.



This thesis describes the characterization and use of a multimodal pH-tunable hydrophobic interaction chromatography media for separating proteins with small structural differences. The media is constructed by attaching copolymer ligands on the surface of spherical agarose support beads. From the multimodal character of the polymeric ligand it is... (More)
Minor structural changes on the surface of proteins can have tremendous impact on their biological properties. When producing therapeutic proteins it is therefore important to verify that the generated protein is the desired one. Small variations such as glycosylations or amino acid substitutions can mean the difference between a functional, defective or even toxic product.



This thesis describes the characterization and use of a multimodal pH-tunable hydrophobic interaction chromatography media for separating proteins with small structural differences. The media is constructed by attaching copolymer ligands on the surface of spherical agarose support beads. From the multimodal character of the polymeric ligand it is possible to allow several interaction types to be in effect at the same time, a property which makes it sensitive to small protein surface modifications. The chromatography media presented here is mainly hydrophobic but will also participate in electrostatic interactions and form hydrogen bonds. In addition, the media will react to the pH of the surrounding solvent and change its hydrophobicity. At low pH it will be more hydrophobic than at neutral pH. Both pH-dependent behavior and how polymer length effects separation have been explored.



The pH-responsiveness of the media provides opportunities to fine-tune the separation process to virtually any protein desirable. This property was used in the work presented here for purifying delicate recombinant hemoglobin. The high selectivity that was obtained from the multimodality was used to separate and investigate the stability of the haptoglobin-hemoglobin complex. Additionally, the media was able to separate glycosylated IgG variants which traditional HIC media could not. This property enabled developing a rapid monitoring method for antibody producing cell systems. (Less)
Please use this url to cite or link to this publication:
author
supervisor
opponent
  • Professor Jungbauer, Alois, University of Natural Resources and Life Sciences, Vienna, Austria
organization
publishing date
type
Thesis
publication status
published
subject
keywords
Hydrophobic interaction chromatography, Multimodal chromatography, Glycosylation, Antibody, Hemoglobin, Protein purification.
pages
134 pages
defense location
Lecture hall C at the Centre for Chemistry and Chemical Engineering, Getingevägen 60, Lund
defense date
2013-04-29 10:30
ISBN
978-91-7473-478-2
language
English
LU publication?
yes
id
8f2bbeac-7f95-4f70-a37b-417163b4e619 (old id 3628843)
date added to LUP
2013-04-03 10:54:09
date last changed
2016-09-19 08:45:16
@phdthesis{8f2bbeac-7f95-4f70-a37b-417163b4e619,
  abstract     = {Minor structural changes on the surface of proteins can have tremendous impact on their biological properties. When producing therapeutic proteins it is therefore important to verify that the generated protein is the desired one. Small variations such as glycosylations or amino acid substitutions can mean the difference between a functional, defective or even toxic product. <br/><br>
<br/><br>
This thesis describes the characterization and use of a multimodal pH-tunable hydrophobic interaction chromatography media for separating proteins with small structural differences. The media is constructed by attaching copolymer ligands on the surface of spherical agarose support beads. From the multimodal character of the polymeric ligand it is possible to allow several interaction types to be in effect at the same time, a property which makes it sensitive to small protein surface modifications. The chromatography media presented here is mainly hydrophobic but will also participate in electrostatic interactions and form hydrogen bonds. In addition, the media will react to the pH of the surrounding solvent and change its hydrophobicity. At low pH it will be more hydrophobic than at neutral pH. Both pH-dependent behavior and how polymer length effects separation have been explored.<br/><br>
<br/><br>
The pH-responsiveness of the media provides opportunities to fine-tune the separation process to virtually any protein desirable. This property was used in the work presented here for purifying delicate recombinant hemoglobin. The high selectivity that was obtained from the multimodality was used to separate and investigate the stability of the haptoglobin-hemoglobin complex. Additionally, the media was able to separate glycosylated IgG variants which traditional HIC media could not. This property enabled developing a rapid monitoring method for antibody producing cell systems.},
  author       = {Kallberg, Kristian},
  isbn         = {978-91-7473-478-2},
  keyword      = {Hydrophobic interaction chromatography,Multimodal chromatography,Glycosylation,Antibody,Hemoglobin,Protein purification.},
  language     = {eng},
  pages        = {134},
  school       = {Lund University},
  title        = {Multimodal Interaction Chromatography for the Investigation and Separation of Proteins},
  year         = {2013},
}