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Different gene expression in immunoglobulin-mutated and immunoglobulin-unmutated forms of chronic lymphocytic leukemia.

Ferrer, Anna; Ollila, Juha; Tobin, Gerard; Nagy, Bálint; Thunberg, Ulf; Aalto, Yan; Vihinen, Mauno LU ; Vilpo, Juhani; Rosenquist, Richard and Knuutila, Sakari (2004) In Cancer Genetics and Cytogenetics1979-01-01+01:002011-01-01+01:00 153(1). p.69-72
Abstract
The mutation status of the immunoglobulin heavy chain variable regions (IgVH) has been found to be a good prognostic indicator for B-cell chronic lymphocytic leukemia (CLL) because unmutated VH genes are associated with rapid disease progression and shorter survival time. To study the differences in gene expression between the Ig-unmutated and Ig-mutated CLL subtypes, we performed gene expression profiling on 31 CLL cases and investigated the VH gene mutation status by sequencing. The array data showed that the greatest variances between the unmutated (20 cases) and the mutated (11 cases) group were in expressions of ZAP70, RAF1, PAX5, TCF1, CD44, SF1, S100A12, NUP214, DAF, GLVR1, MKK6, AF4, CX3CR1, NAFTC1, and HEX. ZAP70 was significantly... (More)
The mutation status of the immunoglobulin heavy chain variable regions (IgVH) has been found to be a good prognostic indicator for B-cell chronic lymphocytic leukemia (CLL) because unmutated VH genes are associated with rapid disease progression and shorter survival time. To study the differences in gene expression between the Ig-unmutated and Ig-mutated CLL subtypes, we performed gene expression profiling on 31 CLL cases and investigated the VH gene mutation status by sequencing. The array data showed that the greatest variances between the unmutated (20 cases) and the mutated (11 cases) group were in expressions of ZAP70, RAF1, PAX5, TCF1, CD44, SF1, S100A12, NUP214, DAF, GLVR1, MKK6, AF4, CX3CR1, NAFTC1, and HEX. ZAP70 was significantly more expressed in the Ig-unmutated CLL group, whereas the expression of all the other genes was higher in the Ig-mutated cases. These results corroborate a recent finding, according to which the expression of ZAP70 can predict the VH mutation status and suggest that RAF1, PAX5, and other differentially expressed genes may offer good markers for differentiating unmutated cases from mutated cases and thus serve as prognostic markers. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
B-Cell: genetics, Chronic, Lymphocytic, Leukemia, Immunoglobulin Heavy Chains: genetics, Immunoglobulin Variable Region: genetics, B-Cell: metabolism, Neoplasm Proteins: biosynthesis, Neoplasm Proteins: genetics, Protein-Tyrosine Kinases: biosynthesis, Protein-Tyrosine Kinases: genetics
in
Cancer Genetics and Cytogenetics1979-01-01+01:002011-01-01+01:00
volume
153
issue
1
pages
69 - 72
publisher
Elsevier
external identifiers
  • PMID:15325098
  • Scopus:4644371975
ISSN
0165-4608
DOI
10.1016/j.cancergencyto.2003.12.016
language
English
LU publication?
no
id
ee9e747e-1218-49b8-90d1-f2bacaac6792 (old id 3635460)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/15325098?dopt=Abstract
date added to LUP
2013-06-12 16:10:32
date last changed
2017-02-19 04:27:31
@article{ee9e747e-1218-49b8-90d1-f2bacaac6792,
  abstract     = {The mutation status of the immunoglobulin heavy chain variable regions (IgVH) has been found to be a good prognostic indicator for B-cell chronic lymphocytic leukemia (CLL) because unmutated VH genes are associated with rapid disease progression and shorter survival time. To study the differences in gene expression between the Ig-unmutated and Ig-mutated CLL subtypes, we performed gene expression profiling on 31 CLL cases and investigated the VH gene mutation status by sequencing. The array data showed that the greatest variances between the unmutated (20 cases) and the mutated (11 cases) group were in expressions of ZAP70, RAF1, PAX5, TCF1, CD44, SF1, S100A12, NUP214, DAF, GLVR1, MKK6, AF4, CX3CR1, NAFTC1, and HEX. ZAP70 was significantly more expressed in the Ig-unmutated CLL group, whereas the expression of all the other genes was higher in the Ig-mutated cases. These results corroborate a recent finding, according to which the expression of ZAP70 can predict the VH mutation status and suggest that RAF1, PAX5, and other differentially expressed genes may offer good markers for differentiating unmutated cases from mutated cases and thus serve as prognostic markers.},
  author       = {Ferrer, Anna and Ollila, Juha and Tobin, Gerard and Nagy, Bálint and Thunberg, Ulf and Aalto, Yan and Vihinen, Mauno and Vilpo, Juhani and Rosenquist, Richard and Knuutila, Sakari},
  issn         = {0165-4608},
  keyword      = {B-Cell: genetics,Chronic,Lymphocytic,Leukemia,Immunoglobulin Heavy Chains: genetics,Immunoglobulin Variable Region: genetics,B-Cell: metabolism,Neoplasm Proteins: biosynthesis,Neoplasm Proteins: genetics,Protein-Tyrosine Kinases: biosynthesis,Protein-Tyrosine Kinases: genetics},
  language     = {eng},
  number       = {1},
  pages        = {69--72},
  publisher    = {Elsevier},
  series       = {Cancer Genetics and Cytogenetics1979-01-01+01:002011-01-01+01:00},
  title        = {Different gene expression in immunoglobulin-mutated and immunoglobulin-unmutated forms of chronic lymphocytic leukemia.},
  url          = {http://dx.doi.org/10.1016/j.cancergencyto.2003.12.016},
  volume       = {153},
  year         = {2004},
}