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Microarray analysis of B-cell stimulation.

Ollila, Juha and Vihinen, Mauno LU (2002) In Vitamines and Hormones 64. p.77-99
Abstract
B-cell development to antibody-producing plasma cells requires the concerted function of a large number of genes and proteins. Genome-level expression profiling during human B-cell maturation was studied in anti-immunoglobulin M-stimulated Ramos cells. cDNA microarrays were used to follow changes in the transcriptome over several days. Close to 1500 genes had significantly altered expression at least at one time point. The genes were organized into clusters based on expression profiles and were further characterized based on the functions of the coded proteins. Several groups of genes important for B cells were analyzed. Here we concentrate on genes involved in signal transduction and cytokines and their receptors. The results provide... (More)
B-cell development to antibody-producing plasma cells requires the concerted function of a large number of genes and proteins. Genome-level expression profiling during human B-cell maturation was studied in anti-immunoglobulin M-stimulated Ramos cells. cDNA microarrays were used to follow changes in the transcriptome over several days. Close to 1500 genes had significantly altered expression at least at one time point. The genes were organized into clusters based on expression profiles and were further characterized based on the functions of the coded proteins. Several groups of genes important for B cells were analyzed. Here we concentrate on genes involved in signal transduction and cytokines and their receptors. The results provide knowledge on the development of humoral immunity. Several new genes were found to be essential for B-cell development. They can be used as targets for research and possibly for drug development. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Antibody Formation: genetics, B-Lymphocytes: chemistry, B-Lymphocytes: metabolism, Cytokines: genetics, Gene Expression Profiling: methods, Gene Expression Regulation: genetics, Lymphocyte Activation: genetics, Oligonucleotide Array Sequence Analysis: methods, Plasma Cells: chemistry, Plasma Cells: metabolism, Plasma Cells: secretion, Signal Transduction: genetics
in
Vitamines and Hormones
volume
64
pages
77 - 99
publisher
Academic Press
external identifiers
  • pmid:11898398
  • scopus:0036365006
ISSN
0083-6729
language
English
LU publication?
no
id
4158f6ad-cb95-457c-a9c6-05728754af0b (old id 3635609)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/11898398?dopt=Abstract
date added to LUP
2013-06-12 16:01:17
date last changed
2017-07-30 04:48:41
@article{4158f6ad-cb95-457c-a9c6-05728754af0b,
  abstract     = {B-cell development to antibody-producing plasma cells requires the concerted function of a large number of genes and proteins. Genome-level expression profiling during human B-cell maturation was studied in anti-immunoglobulin M-stimulated Ramos cells. cDNA microarrays were used to follow changes in the transcriptome over several days. Close to 1500 genes had significantly altered expression at least at one time point. The genes were organized into clusters based on expression profiles and were further characterized based on the functions of the coded proteins. Several groups of genes important for B cells were analyzed. Here we concentrate on genes involved in signal transduction and cytokines and their receptors. The results provide knowledge on the development of humoral immunity. Several new genes were found to be essential for B-cell development. They can be used as targets for research and possibly for drug development.},
  author       = {Ollila, Juha and Vihinen, Mauno},
  issn         = {0083-6729},
  keyword      = {Antibody Formation: genetics,B-Lymphocytes: chemistry,B-Lymphocytes: metabolism,Cytokines: genetics,Gene Expression Profiling: methods,Gene Expression Regulation: genetics,Lymphocyte Activation: genetics,Oligonucleotide Array Sequence Analysis: methods,Plasma Cells: chemistry,Plasma Cells: metabolism,Plasma Cells: secretion,Signal Transduction: genetics},
  language     = {eng},
  pages        = {77--99},
  publisher    = {Academic Press},
  series       = {Vitamines and Hormones},
  title        = {Microarray analysis of B-cell stimulation.},
  volume       = {64},
  year         = {2002},
}