Studies of the reversed α-mannosidase reaction in high concentrations of mannose
(1989) In Enzyme and Microbial Technology 11(6). p.347-352- Abstract
- The reversal of the hydrolytic activity of α-mannosidase from jack bean has been studied in high concentrations of mannose. From an initial concentration of 85% mannose (w/w), a maximum yield of 70% (w/w) di- and oligosaccharides was obtained. The products were separated by gel chromatography and high-performance liquid chromatography, and characterized by gas-liquid chromatographymass spectrometry and 1H-nuclear magnetic resonance spectroscopy. The equilibrium for the formation of di- and oligosaccharides was investigated, and the equilibrium constant for the formation of disaccharides was calculated to be 5.5 ± 0.9. The stability of the enzyme and the rate of formation of disaccharides were studied as functions of temperature, pH, and... (More)
- The reversal of the hydrolytic activity of α-mannosidase from jack bean has been studied in high concentrations of mannose. From an initial concentration of 85% mannose (w/w), a maximum yield of 70% (w/w) di- and oligosaccharides was obtained. The products were separated by gel chromatography and high-performance liquid chromatography, and characterized by gas-liquid chromatographymass spectrometry and 1H-nuclear magnetic resonance spectroscopy. The equilibrium for the formation of di- and oligosaccharides was investigated, and the equilibrium constant for the formation of disaccharides was calculated to be 5.5 ± 0.9. The stability of the enzyme and the rate of formation of disaccharides were studied as functions of temperature, pH, and starting concentration of mannose. It was shown that the enzyme was not inhibited by a total sugar concentration of 83% (w/w), compared with 40%.
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Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/36570176-3de7-4af5-9969-d4af22a281e4
- author
- Johansson, Elisabet ; Hedbys, Lars ; Mosbach, Klaus LU ; Larsson, Per-Olof LU ; Gunnarsson, Alf and Svensson, Sigfrid
- organization
- publishing date
- 1989
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- alpha-mannosidase, reversed hydrolysis, enzyme stability
- in
- Enzyme and Microbial Technology
- volume
- 11
- issue
- 6
- pages
- 6 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:0024676159
- ISSN
- 0141-0229
- DOI
- 10.1016/0141-0229(89)90018-5
- language
- English
- LU publication?
- yes
- id
- 36570176-3de7-4af5-9969-d4af22a281e4
- date added to LUP
- 2024-06-17 14:39:53
- date last changed
- 2024-09-02 15:11:46
@article{36570176-3de7-4af5-9969-d4af22a281e4, abstract = {{The reversal of the hydrolytic activity of α-mannosidase from jack bean has been studied in high concentrations of mannose. From an initial concentration of 85% mannose (w/w), a maximum yield of 70% (w/w) di- and oligosaccharides was obtained. The products were separated by gel chromatography and high-performance liquid chromatography, and characterized by gas-liquid chromatographymass spectrometry and 1H-nuclear magnetic resonance spectroscopy. The equilibrium for the formation of di- and oligosaccharides was investigated, and the equilibrium constant for the formation of disaccharides was calculated to be 5.5 ± 0.9. The stability of the enzyme and the rate of formation of disaccharides were studied as functions of temperature, pH, and starting concentration of mannose. It was shown that the enzyme was not inhibited by a total sugar concentration of 83% (w/w), compared with 40%.<br/><br/>}}, author = {{Johansson, Elisabet and Hedbys, Lars and Mosbach, Klaus and Larsson, Per-Olof and Gunnarsson, Alf and Svensson, Sigfrid}}, issn = {{0141-0229}}, keywords = {{alpha-mannosidase; reversed hydrolysis; enzyme stability}}, language = {{eng}}, number = {{6}}, pages = {{347--352}}, publisher = {{Elsevier}}, series = {{Enzyme and Microbial Technology}}, title = {{Studies of the reversed α-mannosidase reaction in high concentrations of mannose}}, url = {{http://dx.doi.org/10.1016/0141-0229(89)90018-5}}, doi = {{10.1016/0141-0229(89)90018-5}}, volume = {{11}}, year = {{1989}}, }