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Comparative membrane-associated proteomics of three different immune reactions in potato

Burra, Dharani Dhar; Lenman, Marit LU ; Levander, Fredrik LU ; Resjö, Svante LU and Andreasson, Erik (2018) In International Journal of Molecular Sciences 19(2).
Abstract

Plants have evolved different types of immune reactions but large-scale proteomics about these processes are lacking, especially in the case of agriculturally important crop pathosystems. We have established a system for investigating PAMP-triggered immunity (PTI) and two different effector-triggered immunity (ETI; triggered by Avr2 or IpiO) responses in potato. The ETI responses are triggered by molecules from the agriculturally important Phytophthora infestans interaction. To perform large-scale membrane protein-based comparison of these responses, we established a method to extract proteins from subcellular compartments in leaves. In the membrane fractions that were subjected to quantitative proteomics analysis, we found that most... (More)

Plants have evolved different types of immune reactions but large-scale proteomics about these processes are lacking, especially in the case of agriculturally important crop pathosystems. We have established a system for investigating PAMP-triggered immunity (PTI) and two different effector-triggered immunity (ETI; triggered by Avr2 or IpiO) responses in potato. The ETI responses are triggered by molecules from the agriculturally important Phytophthora infestans interaction. To perform large-scale membrane protein-based comparison of these responses, we established a method to extract proteins from subcellular compartments in leaves. In the membrane fractions that were subjected to quantitative proteomics analysis, we found that most proteins regulated during PTI were also regulated in the same way in ETI. Proteins related to photosynthesis had lower abundance, while proteins related to oxidative and biotic stress, as well as those related to general antimicrobial defense and cell wall degradation, were found to be higher in abundance. On the other hand, we identified a few proteins—for instance, an ABC transporter-like protein—that were only found in the PTI reaction. Furthermore, we also identified proteins that were regulated only in ETI interactions. These included proteins related to GTP binding and heterotrimeric G-protein signaling, as well as those related to phospholipase signaling.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Désirée, Effector-triggered immunity, ETI, Potato, Proteomics, PTI
in
International Journal of Molecular Sciences
volume
19
issue
2
publisher
MOLECULAR DIVERSITY PRESERVATION INT
external identifiers
  • scopus:85041946614
ISSN
1661-6596
DOI
10.3390/ijms19020538
language
English
LU publication?
yes
id
36672e13-3c4e-4e3f-a7a8-a5bce88d8a5b
date added to LUP
2018-02-20 14:32:54
date last changed
2018-11-21 21:38:08
@article{36672e13-3c4e-4e3f-a7a8-a5bce88d8a5b,
  abstract     = {<p>Plants have evolved different types of immune reactions but large-scale proteomics about these processes are lacking, especially in the case of agriculturally important crop pathosystems. We have established a system for investigating PAMP-triggered immunity (PTI) and two different effector-triggered immunity (ETI; triggered by Avr2 or IpiO) responses in potato. The ETI responses are triggered by molecules from the agriculturally important Phytophthora infestans interaction. To perform large-scale membrane protein-based comparison of these responses, we established a method to extract proteins from subcellular compartments in leaves. In the membrane fractions that were subjected to quantitative proteomics analysis, we found that most proteins regulated during PTI were also regulated in the same way in ETI. Proteins related to photosynthesis had lower abundance, while proteins related to oxidative and biotic stress, as well as those related to general antimicrobial defense and cell wall degradation, were found to be higher in abundance. On the other hand, we identified a few proteins—for instance, an ABC transporter-like protein—that were only found in the PTI reaction. Furthermore, we also identified proteins that were regulated only in ETI interactions. These included proteins related to GTP binding and heterotrimeric G-protein signaling, as well as those related to phospholipase signaling.</p>},
  articleno    = {538},
  author       = {Burra, Dharani Dhar and Lenman, Marit and Levander, Fredrik and Resjö, Svante and Andreasson, Erik},
  issn         = {1661-6596},
  keyword      = {Désirée,Effector-triggered immunity,ETI,Potato,Proteomics,PTI},
  language     = {eng},
  month        = {02},
  number       = {2},
  publisher    = {MOLECULAR DIVERSITY PRESERVATION INT},
  series       = {International Journal of Molecular Sciences},
  title        = {Comparative membrane-associated proteomics of three different immune reactions in potato},
  url          = {http://dx.doi.org/10.3390/ijms19020538},
  volume       = {19},
  year         = {2018},
}