Antibodies to a 64,000 Mr human islet cell antigen precede the clinical onset of insulin-dependent diabetes
(1987) In Journal of Clinical Investigation 79(3). p.34-926- Abstract
Antibodies in sera from newly diagnosed insulin-dependent diabetes mellitus (IDDM) patients are directed to a human islet cell protein of relative molecular mass (Mr) 64,000. Since IDDM seems to develop after a prodromal period of beta-cell autoimmunity, this study has examined whether 64,000 Mr antibodies could be detected in 14 individuals who subsequently developed IDDM and five first degree relatives who have indications of altered beta-cell function. Sera were screened by immunoprecipitation on total detergent lysates of human islets and positive sera retested on membrane protein preparations. Antibodies to the 64,000 Mr membrane protein were consistently detected in 11/14 IDDM patients, and in all 5 first degree relatives. 10 IDDM... (More)
Antibodies in sera from newly diagnosed insulin-dependent diabetes mellitus (IDDM) patients are directed to a human islet cell protein of relative molecular mass (Mr) 64,000. Since IDDM seems to develop after a prodromal period of beta-cell autoimmunity, this study has examined whether 64,000 Mr antibodies could be detected in 14 individuals who subsequently developed IDDM and five first degree relatives who have indications of altered beta-cell function. Sera were screened by immunoprecipitation on total detergent lysates of human islets and positive sera retested on membrane protein preparations. Antibodies to the 64,000 Mr membrane protein were consistently detected in 11/14 IDDM patients, and in all 5 first degree relatives. 10 IDDM patients were already positive in the first samples, obtained 4-91 mo before the clinical onset of IDDM, whereas 1 patient progressed to a high 64,000 Mr immunoreactivity, at a time where a commencement of a decline in beta-cell function was detected. 64,000 Mr antibodies were detected before islet cell cytoplasmic antibodies (ICCA) in two patients. In the control groups of 21 healthy individuals, 36 patients with diseases of the thyroid and 5 SLE patients, the 64,000 Mr antibodies were detected in only one individual, who was a healthy sibling to an IDDM patient. These results suggest that antibodies against the Mr 64,000 human islet protein are an early marker of beta-cell autoimmunity and may be useful to predict a later development of IDDM.
(Less)
- author
- Baekkeskov, S
; Landin, M
LU
; Kristensen, J K
; Srikanta, S
; Bruining, G J
; Mandrup-Poulsen, T
; de Beaufort, C
; Soeldner, J S
; Eisenbarth, G
; Lindgren, F
; Sundquist, Göran
and Lernmark, Ake
LU
(Less) - organization
- publishing date
- 1987
- type
- Contribution to journal
- publication status
- published
- keywords
- Adolescent, Adult, Autoantibodies/immunology, Autoantigens/immunology, Cytoplasm/immunology, Diabetes Mellitus, Type 1/genetics, Female, Humans, Immunosorbent Techniques, Islets of Langerhans/immunology, Lupus Erythematosus, Systemic/immunology, Male, Membrane Proteins/immunology, Middle Aged, Molecular Weight, Prospective Studies, Thyroid Diseases/immunology
- in
- Journal of Clinical Investigation
- volume
- 79
- issue
- 3
- pages
- 34 - 926
- publisher
- The American Society for Clinical Investigation
- external identifiers
-
- scopus:0023090843
- pmid:3546382
- ISSN
- 0021-9738
- DOI
- 10.1172/JCI112903
- language
- English
- LU publication?
- yes
- id
- 366fb20e-1e1d-4e95-b91a-f7350d6d1b0e
- date added to LUP
- 2021-09-17 15:58:02
- date last changed
- 2024-04-20 13:37:25
@article{366fb20e-1e1d-4e95-b91a-f7350d6d1b0e,
abstract = {{<p>Antibodies in sera from newly diagnosed insulin-dependent diabetes mellitus (IDDM) patients are directed to a human islet cell protein of relative molecular mass (Mr) 64,000. Since IDDM seems to develop after a prodromal period of beta-cell autoimmunity, this study has examined whether 64,000 Mr antibodies could be detected in 14 individuals who subsequently developed IDDM and five first degree relatives who have indications of altered beta-cell function. Sera were screened by immunoprecipitation on total detergent lysates of human islets and positive sera retested on membrane protein preparations. Antibodies to the 64,000 Mr membrane protein were consistently detected in 11/14 IDDM patients, and in all 5 first degree relatives. 10 IDDM patients were already positive in the first samples, obtained 4-91 mo before the clinical onset of IDDM, whereas 1 patient progressed to a high 64,000 Mr immunoreactivity, at a time where a commencement of a decline in beta-cell function was detected. 64,000 Mr antibodies were detected before islet cell cytoplasmic antibodies (ICCA) in two patients. In the control groups of 21 healthy individuals, 36 patients with diseases of the thyroid and 5 SLE patients, the 64,000 Mr antibodies were detected in only one individual, who was a healthy sibling to an IDDM patient. These results suggest that antibodies against the Mr 64,000 human islet protein are an early marker of beta-cell autoimmunity and may be useful to predict a later development of IDDM.</p>}},
author = {{Baekkeskov, S and Landin, M and Kristensen, J K and Srikanta, S and Bruining, G J and Mandrup-Poulsen, T and de Beaufort, C and Soeldner, J S and Eisenbarth, G and Lindgren, F and Sundquist, Göran and Lernmark, Ake}},
issn = {{0021-9738}},
keywords = {{Adolescent; Adult; Autoantibodies/immunology; Autoantigens/immunology; Cytoplasm/immunology; Diabetes Mellitus, Type 1/genetics; Female; Humans; Immunosorbent Techniques; Islets of Langerhans/immunology; Lupus Erythematosus, Systemic/immunology; Male; Membrane Proteins/immunology; Middle Aged; Molecular Weight; Prospective Studies; Thyroid Diseases/immunology}},
language = {{eng}},
number = {{3}},
pages = {{34--926}},
publisher = {{The American Society for Clinical Investigation}},
series = {{Journal of Clinical Investigation}},
title = {{Antibodies to a 64,000 Mr human islet cell antigen precede the clinical onset of insulin-dependent diabetes}},
url = {{http://dx.doi.org/10.1172/JCI112903}},
doi = {{10.1172/JCI112903}},
volume = {{79}},
year = {{1987}},
}