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Nitrate modulates stem cell dynamics in Arabidopsis shoot meristems through cytokinins

Landrein, Benoit; Formosa-Jordan, Pau; Malivert, Alice; Schuster, Christoph; Melnyk, Charles W.; Yang, Weibing; Turnbull, Colin; Meyerowitz, Elliot M.; Locke, James C.W. and Jönsson, Henrik LU (2018) In Proceedings of the National Academy of Sciences of the United States of America 115(6). p.1382-1387
Abstract

The shoot apical meristem (SAM) is responsible for the generation of all the aerial parts of plants. Given its critical role, dynamical changes in SAM activity should play a central role in the adaptation of plant architecture to the environment. Using quantitative microscopy, grafting experiments, and genetic perturbations, we connect the plant environment to the SAM by describing the molecular mechanism by which cytokinins signal the level of nutrient availability to the SAM. We show that a systemic signal of cytokinin precursors mediates the adaptation of SAM size and organogenesis rate to the availability of mineral nutrients by modulating the expression of WUSCHEL, a key regulator of stem cell homeostasis. In time-lapse... (More)

The shoot apical meristem (SAM) is responsible for the generation of all the aerial parts of plants. Given its critical role, dynamical changes in SAM activity should play a central role in the adaptation of plant architecture to the environment. Using quantitative microscopy, grafting experiments, and genetic perturbations, we connect the plant environment to the SAM by describing the molecular mechanism by which cytokinins signal the level of nutrient availability to the SAM. We show that a systemic signal of cytokinin precursors mediates the adaptation of SAM size and organogenesis rate to the availability of mineral nutrients by modulating the expression of WUSCHEL, a key regulator of stem cell homeostasis. In time-lapse experiments, we further show that this mechanism allows meristems to adapt to rapid changes in nitrate concentration, and thereby modulate their rate of organ production to the availability of mineral nutrients within a few days. Our work sheds light on the role of the stem cell regulatory network by showing that it not only maintains meristem homeostasis but also allows plants to adapt to rapid changes in the environment.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Arabidopsis, Cytokinin hormones, Plant development, Plant nutrition, Shoot apical meristem
in
Proceedings of the National Academy of Sciences of the United States of America
volume
115
issue
6
pages
6 pages
publisher
National Acad Sciences
external identifiers
  • scopus:85041526225
ISSN
0027-8424
DOI
10.1073/pnas.1718670115
language
English
LU publication?
yes
id
36704768-56fa-43d9-ac1d-b1222cf97c3f
date added to LUP
2018-02-21 08:25:20
date last changed
2018-07-15 04:47:34
@article{36704768-56fa-43d9-ac1d-b1222cf97c3f,
  abstract     = {<p>The shoot apical meristem (SAM) is responsible for the generation of all the aerial parts of plants. Given its critical role, dynamical changes in SAM activity should play a central role in the adaptation of plant architecture to the environment. Using quantitative microscopy, grafting experiments, and genetic perturbations, we connect the plant environment to the SAM by describing the molecular mechanism by which cytokinins signal the level of nutrient availability to the SAM. We show that a systemic signal of cytokinin precursors mediates the adaptation of SAM size and organogenesis rate to the availability of mineral nutrients by modulating the expression of WUSCHEL, a key regulator of stem cell homeostasis. In time-lapse experiments, we further show that this mechanism allows meristems to adapt to rapid changes in nitrate concentration, and thereby modulate their rate of organ production to the availability of mineral nutrients within a few days. Our work sheds light on the role of the stem cell regulatory network by showing that it not only maintains meristem homeostasis but also allows plants to adapt to rapid changes in the environment.</p>},
  author       = {Landrein, Benoit and Formosa-Jordan, Pau and Malivert, Alice and Schuster, Christoph and Melnyk, Charles W. and Yang, Weibing and Turnbull, Colin and Meyerowitz, Elliot M. and Locke, James C.W. and Jönsson, Henrik},
  issn         = {0027-8424},
  keyword      = {Arabidopsis,Cytokinin hormones,Plant development,Plant nutrition,Shoot apical meristem},
  language     = {eng},
  month        = {02},
  number       = {6},
  pages        = {1382--1387},
  publisher    = {National Acad Sciences},
  series       = {Proceedings of the National Academy of Sciences of the United States of America},
  title        = {Nitrate modulates stem cell dynamics in Arabidopsis shoot meristems through cytokinins},
  url          = {http://dx.doi.org/10.1073/pnas.1718670115},
  volume       = {115},
  year         = {2018},
}